| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 710,173 | A→G | 12.0% | intergenic (+57/+27) | chiQ → / ← fur | lipoprotein ChiQ/DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,173 | 0 | A | G | 12.0% | 106.4 / 6.5 | 52 | intergenic (+57/+27) | chiQ/fur | lipoprotein ChiQ/DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base A (23/20); new base G (3/3); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01 | |||||||||||
CAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCA > NC_000913/710037‑710305 | cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg > 1:477657/1‑143 (MQ=255)cAACGCAAACCGGAAATAATCAGGCGGTGAAAGCCGACTGTGATACAGTCTGGCAGGAAAT‑ACGCAGTAATCACAAATAAATGAGAGCTGTAAGTCTCGCTTTGCTTATTCACCACGCACAAACACGACAAGCGGCAGGTCgg > 2:891528/1‑143 (MQ=255) aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCt < 1:113524/143‑1 (MQ=255) aCGCAAACCGGAAATGAGCGGGCGGTGGAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt < 2:297776/143‑1 (MQ=255) aCGCAAACCGGAAATGACCAGGCCGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATCTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt < 2:107709/143‑1 (MQ=255) cGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCGCGCTTTGCTTATGTAACAAACAAATATTTGTCAACCCGCAGGTTGGCtt > 1:840278/1‑143 (MQ=255) gCAAACCGGAAATGATCAGGCGGTCAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCGTGCATAAAAAAGCCAACCTGCGGGTTGGCttt < 2:496154/143‑1 (MQ=255) cAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGCCtttt > 2:750682/1‑143 (MQ=255) cAAACCGGAAATGAGCAGTCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAAGAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtttt < 1:176673/143‑1 (MQ=255) aaaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc < 1:812316/143‑1 (MQ=255) aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct > 1:220415/1‑143 (MQ=255) aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct > 2:266569/1‑143 (MQ=255) gtGATCAGGCGGTCACAGCCGATTGTGATCAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAgg < 2:196180/142‑1 (MQ=255) gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCCGGTTGGCTTTTCTCGTTCAGGCTGGCTTa > 1:650441/1‑143 (MQ=255) gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa > 1:200853/1‑143 (MQ=255) gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTGTCTTATTTCCCCTGCTTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa > 1:735688/1‑143 (MQ=255) ggTGAAAGCCGATTGTGATAAAATAT‑GCATTTATTGGCGCTCGAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAGAACAGCCAACCCGCAGGTTCGCTTTTCTCGTTCAGGCTGGCTTact > 2:925154/1‑141 (MQ=255) aGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATCACAAATAAGTGAGAGGTGTAACTCTCGCTGTTCTTATTTCTCTTGCATACAAAAGACAACCCGCTGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTAGCCtt > 2:347126/1‑143 (MQ=255) gCCTATTGTGATTAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATATGTGATAGCTGTAACTGTCGCGTTTCTTATTTCCCTTGCATAAAAAAGCCATCCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTc < 2:783589/143‑1 (MQ=255) gCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTc > 2:427615/1‑143 (MQ=255) gtgATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa < 1:904119/143‑1 (MQ=255) gtgATAAAGTCGGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa < 2:309783/143‑1 (MQ=255) gATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCGGGTTGGCTTTTCTCGTTCAGTCTGGCTTATTTGCCTTCGTGCGCATg > 1:704670/1‑143 (MQ=255) aTAAAGTCTGTCGGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAGTTGCCTTCGTGCGCATGt > 2:719687/1‑143 (MQ=255) aGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCAt < 2:118080/143‑1 (MQ=255) ggCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg < 1:158521/143‑1 (MQ=255) ggCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg < 2:719150/143‑1 (MQ=255) gCACGAAAT‑ACGCAGCAATAACAAGTAAGGGAGAGCTGTAACTCTCGCTGTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCGTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc < 2:300095/143‑1 (MQ=255) cAGGAAAT‑ACGCAGTAATAACAAATGAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGGTGGCTTTTCGCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg < 1:585908/143‑1 (MQ=255) cAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTGATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg < 2:399334/143‑1 (MQ=255) aaT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCCGGCTGGCTTATTTGCCTTCGTGCGCATTTTCATCTTCGCTGCAAt > 1:240598/1‑143 (MQ=255) t‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGACTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg > 1:914666/1‑143 (MQ=255) aaCAAATGAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca < 1:648039/143‑1 (MQ=255) aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt > 1:378213/1‑98 (MQ=255) aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt < 2:378213/98‑1 (MQ=255) actaggTGAGCCCTGTAACTCTCCCTATACTTCTTGCCCTTGCATAAAACAGCCAACCCCCAGGTTGGCGTTTCTCGTTCAGGCTCGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa < 1:31048/138‑1 (MQ=255) aTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGTCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc < 1:419530/143‑1 (MQ=255) gagaGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCTCTGGTTGGCTTTTCTTGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa < 1:36643/143‑1 (MQ=255) cTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag < 1:216792/143‑1 (MQ=255) tcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTgg < 2:38891/143‑1 (MQ=255) gCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTACCGATAGAGTCTGTGCtt > 1:114241/1‑143 (MQ=255) gCTTTTCTTATTTCCCTTGCATAAAAAAGCAAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCCCAGTGACCGTACAGATCGAGACTGTGGtc > 2:512858/1‑142 (MQ=255) ttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACTGTATAGATAGAGACTGTGGTTAGTc > 2:724764/1‑143 (MQ=255) cTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCCTCTTCGCGGCAATCGCCTTCGGCACAGTGGCCGTACAGATAGATACTGTGGTTAGTGAg > 2:461173/1‑143 (MQ=255) ttATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAgg > 1:508529/1‑143 (MQ=255) aTTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTCTTTGCCTTCGTGCGCATGTTCATCTTCGCGGAAACCGCCTTTGGCTCAGTGGTCGTAACCATACAGACTCTGCTTAGTCAGGCg > 1:586470/1‑143 (MQ=255) ttGCATAACAGAGCCAACCCGCAGGTTGGCTTTGCTCGTTCAGGCTGGCTTATTTGCCTTCGGGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCa < 2:28473/143‑1 (MQ=255) ttGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCa > 1:878948/1‑143 (MQ=255) tAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTGGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGttt > 1:13681/1‑143 (MQ=255) aaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGc > 1:877631/1‑143 (MQ=255) aaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCg > 1:169855/1‑143 (MQ=255) gCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTCGCCTTCGTGCGCATGTTCATCTTCGCGGCACTCGCCTTCGGCACAGTGACCGTAAAGATAGCGCCGGTGGTTAGTCAGGCGCATGCCACGCTTAGCGGCa > 2:325684/1‑143 (MQ=255) | CAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCA > NC_000913/710037‑710305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |