| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | 100% | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 98.6% | 105.4 / ‑6.6 | 39 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (18/19); minor base C (2/0); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759120‑759387 | ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATCACCAGCACCGAACAAAAACCCCGGATCCAACa > 2:434856/1‑143 (MQ=255)ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGACGAGAAACCCTGGATccacca > 2:255544/1‑143 (MQ=255)ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACTCCGGAACCAACa > 2:405926/1‑143 (MQ=255) ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGa < 1:601547/143‑1 (MQ=255) ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGACTGGTGCCGAATATATGCACAATACAAGCACCGAAAAACAACTCTGGATCAAACAGcatcacgc > 2:552201/1‑136 (MQ=255) gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg > 1:631628/1‑143 (MQ=255) gTTCATTCGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg < 2:125808/143‑1 (MQ=255) ttctgctGCCAGCGGCAAATAAACCACGCACCTGGGCGAGCTGCGGGACGCCCTCAAGCAAACCTACTGCGGCCCGATGAGTGCCGAGTATATGCACATTACATGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt < 2:647946/137‑1 (MQ=255) ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAgg > 1:662571/1‑142 (MQ=255) aTTTGCCAGCGGCAAAGAAGCCAGGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTg < 2:398839/143‑1 (MQ=255) tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg > 1:243113/1‑143 (MQ=255) tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg < 1:295919/143‑1 (MQ=255) cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACCGCGTATCGAGTCTGGTcgcg > 2:731055/1‑143 (MQ=255) gAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGAATCCAACAGCGTATCGAGTCTGGTCGCGCGCCTTTCaa > 1:637280/1‑143 (MQ=255) aaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCACGCAAACCTGCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATa < 1:529281/143‑1 (MQ=255) aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg < 1:134456/143‑1 (MQ=255) aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg < 1:906029/143‑1 (MQ=255) gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc > 2:294336/1‑117 (MQ=255) gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 1:294336/117‑1 (MQ=255) ggCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTGCCGAGTATATGCACACTACCAGCACCGAAGCAACACTCTGGCTCCAACACCGCCCCGAGTCTCGCCGCGCCACCTTCAATAGCGACGAGaaaaa > 2:218673/1‑143 (MQ=255) gAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACg < 1:183932/143‑1 (MQ=255) aGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACACTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGc < 1:158733/143‑1 (MQ=255) aGCCGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGc < 1:239124/143‑1 (MQ=255) gctgcgGGAAGCCCGCAAGCAAGCCTACGGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCAAACAGCGTATCGAGTCTGGTCGCGCGACTGTCAATAGCGAAGAGAAAAAACGct < 2:815028/143‑1 (MQ=255) ctgGAAGCCCCAGAGCCAAGCTCCTGCGGCCAGTGGGGTGCCGGGTATATGCACAGTACCAGTACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt < 1:391899/143‑1 (MQ=255) gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGAACATTACCAGCCCAGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGAGCGACTTTGCAGAGCGAAGAGAAAAAACGCTTCTTAAg > 2:930245/1‑143 (MQ=255) aaccAAACCTACTGCGCCAGCATTGTTGCCGCGCTGGTCCGCATGCCCAGCACCGAAGAAAAACTCTGGATCTAACAGCGTATCTAGTCTGTTCGCGCGACTTCCAATACCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:914618/140‑1 (MQ=255) cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTg > 1:781299/1‑143 (MQ=255) tgcggCGGGCCGGTTGCGGCCGGGGATATGCACATTACCAGCAGCGAAGAAAGGCTCTGGATCCGACAGGGTAGCGAGGCTGGTCGCGCGACGTGCAATGGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGa < 2:306632/139‑1 (MQ=255) ccgCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 1:712765/141‑1 (MQ=255) cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGGGTATCGACTCTGGCCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGATGaaagac > 2:236222/1‑139 (MQ=255) cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGGCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGCAGGTCTTg > 1:353947/1‑143 (MQ=255) tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGCAGGTCTTGAACGTTAcc > 1:145842/1‑143 (MQ=255) aGTATGTGCACATAACCAGCAGCAAAGAAAAGCTCTGGATCCAAGAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc < 2:513090/143‑1 (MQ=255) acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGCCTTTCAAGAGCGAAGAGAAAAGACGCATCGTAGCCGAACTGACCGCCGCTGAAGGTCTTGACCGTTACCTCGGCCCaagcttc > 1:215334/1‑138 (MQ=255) cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaa > 1:679755/1‑143 (MQ=255) gaagaaACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGCTACCTCGGCGCAAAATTCCCTGGCGCAAAACg < 1:221745/143‑1 (MQ=255) gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCACAACg > 1:594982/1‑143 (MQ=255) aaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCATGACAGATAACCCTGCGCACAACTCCCTGGCACAAAACGCAtct > 2:661596/1‑143 (MQ=255) | TTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759120‑759387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |