| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,018,983 | A→T | 9.5% | intergenic (+46/+30) | matP → / ← ompA | macrodomain Ter protein/outer membrane porin A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,018,983 | 0 | A | T | 9.5% | 149.0 / 6.2 | 61 | intergenic (+46/+30) | matP/ompA | macrodomain Ter protein/outer membrane porin A |
| Reads supporting (aligned to +/- strand): ref base A (25/29); new base T (4/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01 | |||||||||||
CTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAG > NC_000913/1018848‑1019124 | cTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCCAACAAAACCCCGCAGCAGCgg > 2:141032/1‑143 (MQ=255) ttGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATACCTGACGAAAGTCAGTTCAATTTACTAAAGGCAAACAAAACCCCGCAGCAGCGGGGtg > 2:506203/1‑142 (MQ=255) ttGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCTGCTGCGGGGtt < 1:176884/143‑1 (MQ=255) atGAAGGTGCGGAAAACAAAAAGAAATACGCGAATAAAATGTCTTCACTGAAGCAGGATCTGCAGGCATTGATGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGtt < 2:851910/142‑1 (MQ=255) aGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTc > 1:454008/1‑143 (MQ=255) aTGCGGAGAGCAAAGGGAGATTCGCGAGTGACATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGCTCCGGAATAACTGACGAAAGTCAGTTCAATTTACTACAGGCAAAAAAAACCCCGCCGCAGCGGGGTTTTTCTa < 2:21599/143‑1 (MQ=255) tGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGATGGGCAACCAATAACTGACGCAAGTGAGATCAAGTTACTAAAGGCAAAAAAAACCCAGCAGCAGCCGCGTTTTGCTAc > 2:509929/1‑143 (MQ=255) ggAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATGGATGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTGTTCTACCAg > 2:343566/1‑143 (MQ=255) gAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGa < 2:165293/143‑1 (MQ=255) aaaaCAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGACTGTCTACCAGAc > 2:190489/1‑143 (MQ=255) aaGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTCCCAGACGCGAAc < 1:943619/143‑1 (MQ=255) aaGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAAc < 1:22142/143‑1 (MQ=255) aaaTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTacg > 1:403811/1‑141 (MQ=255) cgcagtaaaTGTCTTCGCGGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCGGTGCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCgg < 2:170064/137‑1 (MQ=255) tgatgcgtcgtcTTTGAAGCAGGTCCTGTGTGCATTGCTGGGTAATGAATAACTGTCGCAAGTCAGTGCAAGTTAGTAAAGGCAAAAAAAGCCCCGCAGCAGCGGGGTTTTTCTACCGGACGAGAACTTAAGCCTGCGGCTGa < 2:778303/137‑1 (MQ=255) tagagtGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa < 2:772069/138‑1 (MQ=255) cacaaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGCTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa < 1:533618/140‑1 (MQ=255) aTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGATCTGGCATCCCCACAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTa > 1:938074/1‑143 (MQ=255) tcttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGCGTGACaa > 1:701468/1‑143 (MQ=255) ttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGAGGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCGGAGTTACAACg > 2:66098/1‑143 (MQ=255) tctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGTAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCt < 1:407566/143‑1 (MQ=255) tGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCttt > 1:870669/1‑143 (MQ=255) cAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAc > 1:422417/1‑143 (MQ=255) aGGAGCGGCCGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc < 1:124691/143‑1 (MQ=255) cTGCAGGCATTGCTGGGTAAGGAAGAACTGACGAAAGTCAGTTCAAGTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTaa < 1:850646/143‑1 (MQ=255) gCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAACCACCAAGACCAGCGGGGTTTTTCGACCAGACTATAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACt < 1:217754/143‑1 (MQ=255) cAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGGTCAAGTTACTAAAGGCGAAAGAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTCATACCTTTAACtt < 2:290519/143‑1 (MQ=255) aGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTc < 2:830939/143‑1 (MQ=255) cATTGGTGGGGACGCAAGAACTGACGACAGTCAGTTCAATTTACTAAAGGCAAAAAAACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt < 1:358514/143‑1 (MQ=255) cATGGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAACACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt < 2:255996/143‑1 (MQ=255) aaggaaaGGAATAACAGACGAAAGTCAGTACAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAc < 1:196663/138‑1 (MQ=255) ggTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAcgc < 1:864667/143‑1 (MQ=255) tAAGGACTATCTGACGAAATTCAGTTCAATTTACTAAGGGCAAAAAAAACCCCGCAGCCGCGGTGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcga < 2:77590/143‑1 (MQ=255) ggAATAACTGACGAAAGTCGGGTCAATTTACTCCCGGCAGCAAATACCTCGCTGCAGCGGCGTTGTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcgacga < 2:915220/143‑1 (MQ=255) aCTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGa > 2:93376/1‑143 (MQ=255) aCGACAGTTAGTTCAGTTTCCTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTGTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCa < 2:358217/143‑1 (MQ=255) aGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGa < 1:42974/143‑1 (MQ=255) ttCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATCCCTTTATCTCCGAACACTACGACACCATCCGGAGCCAGGCACTCGATc > 2:914945/1‑143 (MQ=255) tCAATTTACTAAAGGCAAAAAAAGCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCa < 2:181119/143‑1 (MQ=255) tCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGGCGATCCGGAGCCAGGCAGTCGCTCa > 1:246472/1‑143 (MQ=255) tCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTCTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCa < 1:66098/143‑1 (MQ=255) gaggttACTGAAGGCAAAAAGAGCCGCGCAGCAGCGGGGTTTTGCTACCAGACGAGGGCTGAAGCCTGCGGCGGGGGTACAACGGCTTTGATACCGGTAACTTCGGTCGCTACGCGACGATCCGGAGCCAGGCAGTCGATCAg < 2:216833/139‑1 (MQ=255) cAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGGTCCGGCGCCAGGCAGTCGATCAg > 1:877425/1‑143 (MQ=255) cAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAg > 1:399744/1‑143 (MQ=255) aaTTTACAAAAGGTAAAAAAAACCCCGCAGCAGCGGTGTTTTTCTACCAGACGAGAATTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGt < 1:551795/143‑1 (MQ=255) aTTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTg < 1:720098/143‑1 (MQ=255) gCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGt > 1:518503/1‑143 (MQ=255) aaaaaaagCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGttt < 1:883232/143‑1 (MQ=255) aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGTGTTTAAGCCTGAGGGTGTGTTACAACGTCTTTGATACCTTTCACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGttt > 1:17866/1‑143 (MQ=255) aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGttt < 2:302076/143‑1 (MQ=255) aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTATACGCGACGATCCGGAGCCAGGCAGTCGATCAGAGCAGCACGcaggtt > 2:728669/1‑138 (MQ=255) aaaaaaaCCCCGCAGCAGCGGGGATTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTc > 1:262482/1‑143 (MQ=255) gaaaaaCCCCGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGGCTTTGATACCTTTAACTTCGATCTCTACGCGCCGATCCGGCTCCCGGCCGTCAATCAGTGCAGCGCGCAGTTTCa > 2:31832/2‑143 (MQ=255) aaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTCTCACg > 2:877825/1‑143 (MQ=255) aaaCCCCGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTGTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCCGCCCGCTGTTTCCCGt > 2:833988/1‑143 (MQ=255) ccccGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGGCGATCCGGAGCCAGGCAGTCGATCAGTCCAGCACGCTGTTTCACGTTGt > 2:512642/1‑143 (MQ=255) ccccGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCCGTTTCACGTTGt > 2:829991/1‑143 (MQ=255) cccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGCTCCGGAGCCAGGCAGTCGATCAGTGCAGCGCGCTGTTTCACGTTGTc > 1:531390/1‑143 (MQ=255) ccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGGGTTACAACGTCTTTGATACCTTTAACTTCGCTCTCTACGCGCCGATCCGGAGCCAGGCAGTCGCTCAGTGCCGCACGCTGTTTCACGTTGTcc > 1:408075/1‑142 (MQ=255) ccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAGATTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCAGTTTCACGTTGTca > 2:331287/1‑143 (MQ=255) cGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcac < 1:758314/143‑1 (MQ=255) cagcagCGGGGTCTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAg < 2:142357/143‑1 (MQ=255) | CTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAG > NC_000913/1018848‑1019124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |