Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,220	C→T	100%	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	100.0%	72.2 / NA	30	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196081‑1196312                                                                                                                                              |                                                                                             tcggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATg                                                                                           >  2:348774/1‑143 (MQ=255) tcggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATg                                                                                           >  1:740045/1‑143 (MQ=255)    gtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGGAATATgggg                                                                                        >  1:333758/1‑142 (MQ=255)      atcgCCCCTGGTGCAATCGTCGCTGACGAATGCGCCCTGCTTGAAGCAATCTTCTGTACCG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTg                                                                                      <  2:414147/143‑1 (MQ=255)      atcgCCCCTGGTGCAACCATCGGGTACGAATGCGCCCTGTTGGAAGCCACCCACTGTCCTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAACCCTGCCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTg                                                                                      <  2:632568/143‑1 (MQ=255)        cgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCTGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGa                                                                                    <  2:597414/143‑1 (MQ=255)                  gCAAACATCGGTGACGAATGCGCTCTGTTTGAAGCCACCCTCGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGc                                                                          <  1:765471/143‑1 (MQ=255)                  gCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGc                                                                          >  1:228898/1‑143 (MQ=255)                   cAAACATCGGTGACGAATGCGCCCTGCTTGAGGTCACCCAC‑GTTCTGCCCCCGAAATATGCCGGTCAGTACAACGTAAATCCTGGCTCTAGTATTCGCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCt                                                                         <  2:151606/143‑1 (MQ=255)                      aCAGCGTTGTCGAATGTGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGAc                                                                      <  2:233883/143‑1 (MQ=39)                           ggTGACCACTGCGCCCTGATTAAAACCACCCACGGTACTA‑CGCCGAAATATGACGGTACGTACAAAGTAAATCCTGGCTCTATTATTCTCTCCGATGAGCTGATAGCAG‑GCCGTATTGGTTAGACTGAAGCGGATGAcgcgat                                                                 >  2:411558/1‑138 (MQ=255)                            gTGCCGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgatt                                                                <  1:341716/143‑7 (MQ=38)                                    tGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                        >  2:391714/1‑143 (MQ=255)                                       gcCCTGTTTTAAGCCACCCACGGTACTG‑TGTCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTTTCCGTTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                     <  2:669192/143‑1 (MQ=25)                                              ttGAAGCCAGCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAGTCTTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgc                                              <  2:161489/143‑1 (MQ=25)                                                    cccgccacGGGACGG‑CGCCGAAATAGGCCGGTCAGGACAGAGTAAAGCCTGGCTCTATTGTTCTCTCCGCTGCGATGAT‑GCTGTCCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        <  2:620396/139‑1 (MQ=18)                                                    ccacccacGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCCTATGGGTTCGACTGAAGCGGCTGACCTGATTGTCAAAGCTCTGGAAGGCGCAGTc                                        >  2:248249/1‑143 (MQ=25)                                                     cacccacGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAGTGTTACAGGGATGTGCGGCGCCATCa                                       >  2:608342/1‑143 (MQ=25)                                                       cccacGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                     >  1:281407/1‑142 (MQ=255)                                                       cccacGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                     >  1:510966/1‑142 (MQ=25)                                                         cacGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                   <  2:680079/143‑1 (MQ=25)                                                                  g‑cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGt                          >  2:447845/1‑143 (MQ=18)                                                                    cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTa                         >  2:486870/1‑143 (MQ=18)                                                                     gtcggaaTGTGCCGGTCGGGAGAAAGTAAATGCTGGCTCTATTGTTCTCTCCGCTGGGATGAT‑GCTGCGCCATATGTGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                        <  2:607465/138‑1 (MQ=14)                                                                     gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                        <  2:740045/143‑1 (MQ=18)                                                                       cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCCATCAATGCCAAGACCGTAAct                      >  1:550378/1‑142 (MQ=18)                                                                          aaTATGCCTGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGGTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                   <  1:662734/143‑1 (MQ=17)                                                                                   gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaa          <  2:772900/143‑2 (MQ=14)                                                                                        gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGACGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt     >  1:585599/1‑143 (MQ=14)                                                                                           aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTACCTTATGCCTCCGACCGTCCgat  >  2:844388/1‑143 (MQ=14)                                                                                                                                              |                                                                                             TCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTG‑CGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAT‑GCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196081‑1196312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑