| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 99.7% | 111.1 / ‑7.3 | 50 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (27/20); minor base G (2/0); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGT > NC_000913/125250‑125521 | tCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtg > 1:447441/1‑143 (MQ=255) ggCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCAGCGAGCTGGCGCGTGAGGGTAAGGCTTGTGAACGCTGGAACATGCTGCACGCGCTGGAAACTTCGCGCGTTCAGTATGTGGCTTAGGagatga > 2:433120/1‑143 (MQ=255) gAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCCACCGCTCGACTGCCGGCGCGTCCCGTATATCGCTTAGGttatcgacg > 1:529531/1‑138 (MQ=255) gAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacg > 1:421768/1‑143 (MQ=255) gAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACAGGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacc > 2:486196/1‑142 (MQ=255) gAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGAAGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacg > 2:313491/1‑143 (MQ=255) ttACGGCGTAGGTCCTGACGTTTATAACGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCTCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑Tc < 1:239881/143‑1 (MQ=255) ggCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTGCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGc > 1:542700/1‑143 (MQ=255) ggTTCTGGCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGc < 2:399344/143‑1 (MQ=255) ggTTCTGACGTTGATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGc < 2:484821/143‑1 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATCTCGCTTAGGTGCTGAACGACGC‑CCCGGCCGTGGCCTCTAcc > 2:931002/1‑143 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTAcc > 1:5468/1‑143 (MQ=255) cGTGCCCCCCCTCACCGAGCTGGCCGCTGAGGGTCCGCGCGGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGa < 1:172213/143‑1 (MQ=255) cGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGCTTGTGAACGCTGGAACATGCTGCACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCTTAGGTGGTGAACGACGC‑TCCGGCCGTGGCATCTCCCGACGATATGa > 1:217216/1‑143 (MQ=255) cGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCGTCTACCGACTATATGa > 1:444724/1‑143 (MQ=255) tGATCTCCTTCACCGAGCTGGGGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGaaa < 2:76809/143‑1 (MQ=255) tGACCGCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGaaa < 1:694622/143‑1 (MQ=255) cctcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAGACTCCGGGCGTTCGGTATGTCGCTGAGGTGGTGAAGGAAGC‑TCCGGCGGGGGCAGCTACGGCGTATAGGAGACTg > 1:5969/1‑143 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGtt < 1:376254/143‑1 (MQ=255) gctTCACCGAGCTGGCGCGTGAGGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTc < 1:178393/142‑1 (MQ=255) ttCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGGGATGGACGACGC‑GCCGGCAGTGGCAGCTACCGACTATATGAAACTGTgcgc > 1:533005/1‑143 (MQ=255) cACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTg > 1:858232/1‑143 (MQ=255) ccGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTggg > 1:795986/1‑141 (MQ=255) gctctggcgTGATGGTCAGGATTGCGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCCTCTACCGACGATATGAAACTGTTCGCTGAGCAgg < 2:335329/137‑1 (MQ=255) cTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATCTCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACGATATCAACCTGATCCCTGAGCAGGt > 2:869641/1‑143 (MQ=255) gTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGATCAGCAGGTCCGtttt > 1:928617/1‑140 (MQ=255) gATGGTCAGGATAGTGAACGATGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTGCGTATATCGCGTAGGTGATGAACAACGC‑TCCGGCAGTGGCAGACACCGACTATATGAAACTGGTCGCGTAGCGGGGCCGTACtga > 2:341523/1‑141 (MQ=255) atggggaGGATTGTGAAGGCGGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTAc < 1:908143/137‑1 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTa > 2:736068/1‑143 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTGAGGGGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACCGCACCCTGAGCAGGTCCGTACTTACGTa > 2:367239/1‑143 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCATAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTa > 2:341342/1‑143 (MQ=255) tCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCgtacgtacgtac > 1:697804/1‑143 (MQ=255) gcAGGATTGTGAACGCTGGAACATGCTGCACCCGGTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAc < 2:284856/142‑1 (MQ=255) gaGGGTTGTGAACGCTGGAAGATGCTGCACGCGCTGGCAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAcc < 2:350851/142‑1 (MQ=255) cggAGTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCg < 1:495700/142‑1 (MQ=255) ggATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTGCTTGCGTACCgg > 1:668191/1‑143 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAGACTGTTCGCTGGGCAGGTCCGTACTTACGTACCGGc > 1:449164/1‑143 (MQ=255) ttGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTGTATTGCCTAGGTGCTG‑ACAGCGCAGCCATCACTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACGTACGTACCGGccg > 1:690797/1‑141 (MQ=255) gAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCATAGGTGGTGAACGACGC‑TCCGGCAGTGGCATGTACCGACTATATGAAGCTGTTCGCGGAGCAGGTCCGTACATACGTAGCGGCTgcaga > 2:857603/1‑139 (MQ=255) cGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑CCCGGTAGTGGCATCTACCGACTATAGGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTCGCGACTa > 2:120367/1‑143 (MQ=255) aCATGCTGCACCCGCGGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGCGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTa < 1:478122/143‑1 (MQ=255) gctgcACCCGCTGGAAACTCCGCGCGTTCCGTGTATCGCTTAGGTGATGAACGACGC‑TTCGGAAATGGCATCTACCGACTATATGAAACTGTTCGCTGAGCGGGTCCGTACTTACGTACGGGCTGTCGACTACCGCGTACTgg > 1:117301/1‑143 (MQ=255) cGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 1:569649/143‑1 (MQ=255) tGGAAACTCCGCGCGTTGCGTATATCGCTCAGGTCATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGc < 2:661180/143‑1 (MQ=255) aCTCCGCGCGTTCCGTATATCGCTTAGGTGATGGATGGCGC‑TCCGTCAGGGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCgg < 2:412559/143‑1 (MQ=255) gcgcgTTCTGTAGATCGCTTAGGTGATGACCGTCGT‑TCCGGCAGTGTCATCTACGGTTTTTCTGATACTGTTCTCTTAGCTGGGCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGtt < 2:873167/143‑1 (MQ=255) gcgTTCCGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTcc < 1:915596/143‑1 (MQ=255) cGTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGc < 1:736068/143‑1 (MQ=255) gTATATCGCTTAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGATCGCTAAGCGGTTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTTGTTCCGACAGct > 1:352534/1‑142 (MQ=255) gtatCGCTTAGGCGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTAGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGCTGGCAGCGGGCGTTCCGACAGCCGt < 2:573580/142‑1 (MQ=255) | TCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGC‑TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGT > NC_000913/125250‑125521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |