| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,778,367 | C→G | 8.0% | intergenic (+20/+23) | ydiO → / ← ydiP | putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator YdiP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,778,367 | 0 | C | G | 8.0% | 129.0 / 3.4 | 46 | intergenic (+20/+23) | ydiO/ydiP | putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator YdiP |
| Reads supporting (aligned to +/- strand): ref base C (21/21); new base G (2/2); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 | |||||||||||
AGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTT > NC_000913/1778229‑1778509 | aGGCGCGCGTCGCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTaa < 1:380419/143‑1 (MQ=255) ccccccGCTTCTGGCGTGATGTCCGTAGTGCACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcg < 1:799602/140‑1 (MQ=255) ctcCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTgcgc < 1:785474/143‑1 (MQ=255) ctcCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcgc < 1:604061/143‑1 (MQ=255) tcCCGCTTCTGGCGTGATGTCCGGTGTGATCGTCTCGGCGGCCCTGCATACTAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc < 1:933659/143‑1 (MQ=255) cGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGAGTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAAAAAATAATCTGCAGGCAGCCCAGCTTCTTAACAAACCgcaacgcg > 2:657368/1‑137 (MQ=255) ggggcTGGAGGGATGTGGGCTGGGAACGTAGCGGCGGGGGTACAGACGAAATTCTGATTTACGTAGCAGGTGGGCAGATCCTGAAAGACTATCAGAACAAATAAGCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCa < 1:900076/139‑1 (MQ=255) tGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCACCCAGATTGATcaa > 2:778531/1‑141 (MQ=255) ccGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCAAGATTTATCCAACAAGa > 1:438712/1‑143 (MQ=255) ccGTTGTCAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGa < 1:499752/143‑1 (MQ=255) ccGTCGTGAACGTAGCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGa < 1:501247/143‑1 (MQ=255) tggtgcGTATCTGCGGCGGTACAGCCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACATATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAc < 2:319458/138‑1 (MQ=255) gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAc > 2:691735/1‑143 (MQ=255) gAACGCATCGGCGGCGGTACAGACGAAATTATGATGTACGTAGCAGGGCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCg < 1:927842/143‑1 (MQ=255) tgtgggcggcggGACAGACCAAATTATTATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGa < 2:792935/139‑1 (MQ=255) tATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGa < 1:613603/143‑1 (MQ=255) aTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg < 1:656303/143‑1 (MQ=255) tCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACCCACTGGGGCGACGGATTTATCCAACAAGACTTACCGGTTGAgg > 1:890350/1‑143 (MQ=255) ggcgtcggTACAGTCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACTATCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGac < 1:157416/143‑2 (MQ=255) gcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTc < 1:877477/143‑1 (MQ=255) aCAGATGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAGTCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCCGCTGAGGAAATTCAggaa > 2:628254/1‑140 (MQ=255) gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGCTTGAGCCAACGAGACTTACCGGTTCAGGAAAATCAGATAGGa > 2:505459/1‑143 (MQ=255) aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTACCAACCTGCGCCGCCAGGTTTATCCAACAAGACTTACCGGTTCAGGAGATTCAGATCggct > 2:806765/1‑141 (MQ=255) aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAt > 2:892688/1‑143 (MQ=255) ttATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg > 1:668675/1‑143 (MQ=255) aTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAACTTCAGACAGGATTTCTTGTTCCg > 2:386040/1‑143 (MQ=255) ttACGTAGCAGCTCGGCAGATCCTGAAAGACTATCATAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCa < 2:865321/143‑1 (MQ=255) cAGGTCGGCAGATCCTGAAATACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt < 2:738606/143‑1 (MQ=255) aGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCCAAACAGttt > 2:520189/1‑143 (MQ=255) aGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACCGttt > 1:665063/1‑143 (MQ=255) ggCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACACACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTCGAGGCAACTCCGAGACTCTTGCTTGTTCCGCCAAACAGTTTTTaac > 2:411075/1‑142 (MQ=255) ggCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaaa > 2:431552/1‑143 (MQ=255) aGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTg > 1:622052/1‑143 (MQ=255) aTCCTGCAAGACTATCAGAACAAATACTCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTc < 1:34298/143‑1 (MQ=255) tATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCACCAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGc > 1:161428/1‑143 (MQ=255) tATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCAAACATGACTATCGGCTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTACTCGc > 2:568617/1‑143 (MQ=255) aataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaaca < 1:44018/143‑1 (MQ=255) tCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACCAGTCTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa < 1:249961/143‑1 (MQ=255) aGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAACACAGTTTTTAAATCGTCTACGGTAATCGCTGCGCAAACAACCGACATgg > 2:414113/1‑143 (MQ=255) aGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATgg < 1:811010/143‑1 (MQ=255) ggcggcGCGGCTTGTTAACATGCTGGGGTGCCAGATTTATCCGACAAGTCTTGCCGGTTGAGGAAATTCAGATAGGATTTGTTGTTCCGTATAACAGTTTTTAAATTGTCTACGGTATTCGTTGGGCGAACTACCGACATGgc < 2:358405/143‑1 (MQ=255) gcggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcg > 1:200262/1‑143 (MQ=255) gcggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGTGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcg < 2:462314/143‑1 (MQ=255) gtcgcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCAAACAAGACCTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAACCAGCTTTTAAATTGTCTACGATAAACGCTCGTCGAACACACGACATGGAGCa > 2:737089/3‑143 (MQ=255) ggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCa > 2:199967/1‑143 (MQ=255) gcAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGCGCGAACAACCGACATGGCGCaaag > 1:54520/1‑142 (MQ=255) tcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGtt < 1:778531/143‑1 (MQ=255) cttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGttt < 1:199967/143‑1 (MQ=255) | AGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTT > NC_000913/1778229‑1778509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |