| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,864,758 | G→A | 9.9% | intergenic (+24/+24) | yeaD → / ← yeaE | putative aldose 1‑epimerase YeaD/methylglyoxal reductase YeaE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,864,758 | 0 | G | A | 9.9% | 149.9 / 8.8 | 61 | intergenic (+24/+24) | yeaD/yeaE | putative aldose 1‑epimerase YeaD/methylglyoxal reductase YeaE |
| Reads supporting (aligned to +/- strand): ref base G (28/27); new base A (3/3); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01 | |||||||||||
AAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGG > NC_000913/1864630‑1864896 | acaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAACGTGCCCAATGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCgg < 2:562775/141‑1 (MQ=255)aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg < 1:58478/143‑1 (MQ=255)aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATCTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCgg > 2:65035/1‑143 (MQ=255)aaaaCATTTGTTTGTGGAGAAACGGCCTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGATGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATCCgg < 2:491048/143‑1 (MQ=255)aaaaCATTTGGTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAGCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg < 2:149315/143‑1 (MQ=255) aaCATTTGTTTGTGTAGAAACGGCTGACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca < 2:176971/143‑1 (MQ=255) cATTTGTGTGTGTAGACACGTGTGACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca < 2:85819/143‑1 (MQ=255) aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGCAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac > 1:661786/1‑143 (MQ=255) ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa < 1:647872/143‑1 (MQ=255) gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTTTGCCGGGCGCAAAGCATCCGACACacaaca > 2:809600/1‑143 (MQ=255) tgtAGATACCGCTGACGCCTGAGTCATGCAAAAAATAACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCCACCGGCACAAAACAAcaca > 2:838472/1‑143 (MQ=255) tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACACAACAttaac < 1:881065/143‑5 (MQ=38) ggtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca < 1:162650/142‑1 (MQ=255) gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCAAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCGACCGCCACACAACATCacac > 1:469873/1‑143 (MQ=255) caaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTAGCGCCATCCACTCGCGGTCCGAAACGTTAATTTACCTTAAGGTTCTGCGGCGGGTTCAATGCCTGCGGCACAACACATGCCACCcta > 2:900891/2‑143 (MQ=255) aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGACACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATc < 1:841628/143‑1 (MQ=255) gggttACGCTTCAGAAACGGAAAAAGTGACCAAAGAGCAACCTGCATATTTGGCGCAAGTCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa < 2:823266/140‑1 (MQ=255) cTTACGCTTCAGAAACGCAAAAAGTGACCAAGGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc < 2:720987/143‑1 (MQ=255) ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg > 2:470204/1‑143 (MQ=255) ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg > 1:452659/1‑143 (MQ=255) ttACGCGTCCGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGTCATGCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg < 2:415027/143‑1 (MQ=255) ttACGCATCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg < 1:22919/143‑1 (MQ=255) ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTCATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGgtt > 2:301480/1‑143 (MQ=255) ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGAAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCCGttc > 1:767979/1‑142 (MQ=255) aCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTACCGGGTGCAATGCAACCGGCACACAACATCACACCATAACCACCGCAGTCTCTCCtgc > 2:782101/1‑141 (MQ=255) ttaaaaaTTGACCGAGTAGAAACCTGCACATCTGGCGCAATCCTTTCGCGTTTCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg < 2:935628/141‑1 (MQ=255) gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg < 1:456148/143‑1 (MQ=255) aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCAAACACAATCTCGCCTGGTCCAGCGCCGTTTTTCCttttcct > 1:730466/1‑140 (MQ=255) aGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg < 1:560522/143‑1 (MQ=255) tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa > 2:27250/1‑143 (MQ=255) ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatat < 1:658903/143‑1 (MQ=255) aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatattc > 1:204666/1‑141 (MQ=255) aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc > 1:180167/1‑143 (MQ=255) aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTCCTGGTGCCGGAACTGccccatc > 2:209557/1‑138 (MQ=255) tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGCATTTCCTTATAGTGCCGGATATGCCTTATCCAGCa > 2:169557/1‑143 (MQ=255) tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAACACATTCAGCGTAGTTTTTCCTTTTGGAGCCGGATATGCCTTATCCAGCa > 1:128410/1‑143 (MQ=255) gCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGGTTTTCCTTTTGGTGCCGGCTATGCCTTATCCAGCAt > 1:40213/1‑143 (MQ=255) acaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCgg > 2:298535/1‑75 (MQ=37) acaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCgg < 1:298535/75‑1 (MQ=37) acaGCTGGCGCAATCCATTCGCGTTGCGGACCGTTAATCTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAg < 1:390615/143‑1 (MQ=255) gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCCTCGCTCAtgc > 1:157403/1‑141 (MQ=255) gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATCGCTAATTTCg > 1:797914/1‑143 (MQ=255) gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg < 2:109853/143‑1 (MQ=255) aaTCCATTCGCGTTGCAAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt < 1:808751/143‑1 (MQ=255) gcactCGCGGTGCGCAGCGTTAGGCTACGTCAACGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCCTCTCCGGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa < 2:357034/139‑1 (MQ=255) ccgtgggcgTTGCGAAAGGTTCATGTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCAGACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa < 2:361985/137‑1 (MQ=255) ccaggcgcgTTGCGAAACGTTAATTTGCGTTAATGTTGTGTGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa < 2:6681/138‑1 (MQ=255) ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa < 1:628476/143‑1 (MQ=255) tCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAGCCGGCACACAACATCACACCTTATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa < 2:58131/143‑1 (MQ=255) gcgTTGCGAAACGTTACAGTACTTTTATGTTGTGATGCGGGTGCAATGCATCCGGCACACCACATGACACGCTATCCAGCGCAGTTTTTCATTCTGGTCCCGGATCAGTCTTATTCAGCAGCGCCAATTGCGCTGAAGAAAGt > 2:876572/1‑143 (MQ=255) gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc < 1:599649/143‑1 (MQ=255) gAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTCCTTCCGCCGCAGAACGTTCCACCt > 1:744862/1‑143 (MQ=255) aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca < 1:337221/143‑1 (MQ=255) gTTAATGTTGTGTGCCGGGTGCAATGCCTCCGGCACACAACATCACACCATATCCGGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCGAGCATAGCTCATGCCGCTGAAGAACCTGCAACCCCAAACACATCCGcgc > 2:52570/1‑141 (MQ=255) aaTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCATACTATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtttt < 2:744865/143‑1 (MQ=255) ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTCGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCGGAAGAAAGTTCGCCCTCCAGCCCACCCGCATCttgtcg > 2:290367/1‑141 (MQ=255) tgtgtgCCGGGTGCAATGCATCCGGCACACCACATCACACCATATCCAGCGCAGTTTCTCCTTTTGGTCCCGAATCTGGCTGAGGCAGCACAGCTAATTCCGCTGAAGAACGTTCGACCTCCACCGCCGCCGCCTCTTGTCgg > 2:510543/1‑143 (MQ=255) tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATTGCTCATTCCGCTGAAGAAAGTGCGATCTTAAACACAGCCGCATTTTGTTgg > 2:217527/1‑143 (MQ=255) cccggTGCACTGCATCCGGCACACAACATCACACCATCTCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATgg < 2:382344/140‑1 (MQ=255) ccGGGTGCAATGCATCCAGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCGCAGCCGCATTTTGTTGGACATgg > 1:23251/1‑143 (MQ=255) ccGGATGCATTGCACCCGGCACACAACATTACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACATCAAGCACAGCCGCATTTTGTTGGACATgg > 1:720654/1‑143 (MQ=255) ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg > 1:487890/1‑143 (MQ=255) | AAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGG > NC_000913/1864630‑1864896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |