Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,311,616 C→T 7.0% intergenic (‑82/+30) ftp ← / ← ompC FAD:protein FMN transferase/outer membrane porin C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,311,6160CT 7.0% 135.5 / 2.9 59intergenic (‑82/+30)ftp/ompCFAD:protein FMN transferase/outer membrane porin C
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base T (2/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

AAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG  >  NC_000913/2311484‑2311751
                                                                                                                                    |                                                                                                                                        
aaaGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGcccgt                                                                                                                                >  1:503521/1‑141 (MQ=255)
   gaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCttttt                                                                                                                             <  1:612427/143‑3 (MQ=255)
       agCGCGGCGGCCAGCAGTGCAACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGa                                                                                                                         <  2:887543/143‑1 (MQ=255)
        gctcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCTCTTTTTTcatt                                                                                                                        <  1:914689/140‑4 (MQ=255)
           cgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGAGTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATc                                                                                                                     <  1:563058/143‑1 (MQ=255)
                    gcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAACCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTCTGTTCGATATCAATCGAGAt                                                                                                            >  2:445639/1‑143 (MQ=255)
                    gcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGATCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGAt                                                                                                            >  1:490152/1‑143 (MQ=255)
                         gCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGaa                                                                                                       <  1:441941/143‑1 (MQ=255)
                         gCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGaa                                                                                                       <  1:308924/143‑1 (MQ=255)
                            aCGCGGGTAAAGCTTATTTCCATCCGTGATTATCCTCATGCGAACGGTCGCAAGACTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTg                                                                                                    <  1:852747/143‑1 (MQ=255)
                              gcgGTTAAAGCTTATTTCCATGCGTGATAATCCTCACGACAAAGGGGTTAAGAGTACACCAAAAAACTGTGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGt                                                                                                  <  1:586265/143‑1 (MQ=255)
                                 cgTAAAGCTTATTTCCCTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaaa                                                                                               <  1:423443/142‑1 (MQ=255)
                                  gTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGTCCCGCACCCCCTTTGTTCGATCTCAATCGAGATTAGAACTGGTAAAc                                                                                              <  1:596223/143‑1 (MQ=255)
                                   tAAAGCTTATTTTCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAcc                                                                                             <  1:258437/143‑1 (MQ=255)
                                       gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATAAAAAAAGGGCCCGCAGGACATTTGTTCGAGATAAATCGAAAGTAGAACTGGTAAAcgggac                                                                                         >  2:153377/1‑138 (MQ=255)
                                         ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAccc                                                                                       >  1:718067/1‑143 (MQ=255)
                                         ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAccc                                                                                       >  1:720993/1‑143 (MQ=255)
                                           aTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAACCACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATGAGAACTGGTACACCAGCCCCag                                                                                     >  2:512453/1‑143 (MQ=255)
                                                  tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACg                                                                              <  1:736117/143‑1 (MQ=255)
                                                     gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCTGGGAGGGTCGTAGATCGATATCAATAGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATg                                                                           >  1:432392/1‑143 (MQ=255)
                                                      tGATTATCCTCATGCGAACGGTCGCAAGAGGACACGAAAAAACTGAGTTTGTACGCTGAAAAAACTGAAAAAAGGGCCCGCGGGCCATCTATTCGATATCAATCGAGATGATAACGGGTAAACCCGACCCAGCGCTACGATGt                                                                          >  2:326911/1‑143 (MQ=255)
                                                             cgtCATGCGAACGGTCGCAAGAGGACACCAAAAAACGGAGTTTGTACGCTGAAGACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                   <  1:543673/141‑1 (MQ=255)
                                                              cTCATGCGAACGGTCGCAAGAGTACACCAAAAAGCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGGGCTCCGCTGTTTTCCgtg                                                                  >  1:353835/1‑143 (MQ=255)
                                                               tCATGCGAACGGTCGCAAGAGTGCACCAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGAACAACACACCTTTCGTTCGATAACATTCAGAATTAGAACTGATAGACCATAGCCAGAGCTAATAAGGTATCAgtag                                                                 >  2:510396/1‑141 (MQ=255)
                                                               tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTGGTCCgtgt                                                                 >  1:385645/1‑143 (MQ=255)
                                                               tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGATTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCAATATCAATAGAAATTAAAACTGGCAAACCACACCCAGAACTACACTATTATCaatat                                                                 >  2:550462/1‑139 (MQ=255)
                                                                cATGCGAACGGTCGCGACAGTACACCAAACAACTGAGTTTGTGCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                <  2:686758/143‑1 (MQ=255)
                                                                 aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAACAAGGGCGCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAACAGATCCAGAGCTACGATGGTATCGGTGTTg                                                               >  2:156701/1‑143 (MQ=255)
                                                                     gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGAGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGATCACAGTGTCGATGc                                                           >  2:2717/1‑143 (MQ=255)
                                                                      aaCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCACAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAACCAGAGCTACGATGCTATCAGTATTTATGcc                                                          >  2:683696/1‑143 (MQ=255)
                                                                       aCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCGTTGTTGGTGcct                                                         >  1:760660/1‑142 (MQ=255)
                                                                       aCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                         <  1:264036/143‑1 (MQ=255)
                                                                       aCGGTCGCAAGAGCACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                         <  1:942589/143‑1 (MQ=255)
                                                                       aCGGGCGCAAGAGTACACCAAAAAACTGTGTTGGTACGCTGAAGACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTAACAGTGTTGATGCCa                                                         <  2:195956/143‑1 (MQ=255)
                                                                          gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTACGATATCAATCGAGATTAGAACTGGTAAACCAGACACAGAGCTACGAGGTTATCAGTGTTGATGCCAG‑Cg                                                     >  2:497302/1‑143 (MQ=255)
                                                                            ctcAACAGCACACCAAAAAACTGAGCGTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCCAGATTAGCACTGGTAAACCAGACCTAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTc                                                   <  1:603060/141‑1 (MQ=255)
                                                                             gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATAGCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATGCGTGTTGATGCCAG‑CGtga                                                  >  1:86903/1‑141 (MQ=255)
                                                                             gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTATGTTCGAACTCAATCGAGATTAGACCTGGGGAGCCTGACCCAGAGCTACGACGTTATCAGTGTTGATGCCAG‑CGTCa                                                  >  1:175003/1‑143 (MQ=255)
                                                                             gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCACTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CATcg                                                  >  2:300843/1‑142 (MQ=255)
                                                                              cAAGAGCACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCAc                                                 <  2:496779/143‑1 (MQ=255)
                                                                                       acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCAAGAGTGAACt                                        >  2:310426/1‑143 (MQ=255)
                                                                                        ccgaaaagCTGAGTTTGTACGCGGAAAACAATGAAAAAAGGGCCCACAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTg                                       <  1:892271/140‑1 (MQ=255)
                                                                                        ccAAAAAACTGAGTTGGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTg                                       <  2:235337/143‑1 (MQ=255)
                                                                                         cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTgg                                      >  1:450958/1‑143 (MQ=255)
                                                                                          aaaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGAGCCCGCAGGCCCGTTGTTAGATAGCAATCGAGCATAGAACTGGTAGACCAGACACAGAGCTACGATATTATCAGTGTCGATGCCAG‑CGACACGGCTGAACtcgg                                     >  2:612756/1‑140 (MQ=255)
                                                                                           aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGTTGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGtt                                    >  1:456797/1‑143 (MQ=255)
                                                                                                cTGAGTTTGCACGCTGAAACCAATCAAAAAAGGCCCCGCAGGCCCTTTGTTCTATATCAATCGAGATTAGAACTGCTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTgtcgt                               <  1:21523/143‑1 (MQ=255)
                                                                                                 tGAGTTTGTACGCTGAACATAATGAAATAATGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTgtcgtc                              <  2:528235/143‑1 (MQ=255)
                                                                                                       tGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCcagcag                        <  2:719642/143‑1 (MQ=255)
                                                                                                              cgAAAACAATGAACAAAGGGCCCGCAGGCCCTTTGTTCCATATCCACTCACATTAGACCTGCTAGGCCATGCCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                 <  1:587708/142‑1 (MQ=255)
                                                                                                                aaaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt               <  1:595537/143‑1 (MQ=255)
                                                                                                                  aaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGGCCAGCAGGTTGATTTTGt             >  1:368814/1‑143 (MQ=255)
                                                                                                                     aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTAACGAGTGAAATAGTTGTCGTCCAGCAGGCTGGTTTTGTAGt          >  1:827787/1‑143 (MQ=39)
                                                                                                                        gAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGGTTTTGTAGTCaa       >  2:221058/1‑143 (MQ=255)
                                                                                                                         aacaaaGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGTCGT‑AATATAGTGGTGGTAGTCGTCCTGCAGGTGGATTTTGTAGTCAAc      >  1:682567/4‑143 (MQ=255)
                                                                                                                           caaaGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGCTGGACTTCGTAGTCACCGt    >  2:810391/2‑143 (MQ=255)
                                                                                                                           agaaGGGCACCCAGGCCCTTTGTTCGATATCAATCGAGCTTAGAGCTGGTGAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACGGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt    <  1:783912/141‑1 (MQ=255)
                                                                                                                           aaacGGCCCCGCAGGCCCCTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt    <  1:584991/143‑1 (MQ=255)
                                                                                                                             aaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGCTTTTGTAGTCAACGTAg  >  1:916394/1‑143 (MQ=255)
                                                                                                                                    |                                                                                                                                        
AAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG‑CGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG  >  NC_000913/2311484‑2311751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑