| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,641,354 | C→A | 50.5% | E151* (GAA→TAA) ‡ | ispG ← | (E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,641,354 | 0 | C | A | 50.5% | ‑0.0 / 48.4 | 52 | E151* (GAA→TAA) ‡ | ispG | (E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin) |
| Reads supporting (aligned to +/- strand): ref base C (10/15); new base A (14/11); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
TTTTGCCAGCAAACGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCA > NC_000913/2641214‑2641491 | ttttGCCAGCAAACGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCAGCGGCGTCGGTTCGc > 1:582560/1‑143 (MQ=255) tggCCAGCAACCGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCa < 2:489119/141‑1 (MQ=255) cAGCAAACGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCGACGCCTGCGGCGTCGGTTCGCCATACt > 1:163450/1‑143 (MQ=255) cAGCAAACGATAAGACTCAACAGCGAGGAAGACGCCAGACGCTTTCACGCTGACTTTGAACTGACCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGAAGATGCCAGCAACGCCCGCGCCGTCGGGAAGCCATAca > 2:931106/1‑142 (MQ=255) cAAACGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACtttt < 2:183411/143‑1 (MQ=255) aaaCGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGTGATGCACAACATTACGCATGGCAGATTCCAGCACCGCCTGCGGCGTCGGTAAGCCCTACTTGTc > 1:433284/1‑143 (MQ=255) aaaCGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTAACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTc < 2:831116/143‑1 (MQ=255) cGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTg < 1:707039/143‑1 (MQ=255) gATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAACTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGACTGCGGCGTCGGTTGCCCATACctttatctc > 2:42850/1‑138 (MQ=255) tCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGGAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCACCGCCTGCGGCGTCGGTAAGCCACGCTTTTCTTGCACACCttt > 2:514064/1‑143 (MQ=255) tCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCttt > 1:628548/1‑143 (MQ=255) acggggAGGAAGACGTCCGACGCTGTCCCGCTGAATTTGAACTGCTCGACGTTCAGGCGGCCGAGTCGACCCGCATGACGCATGGCAGATTCCAGCCGCGAGTGTCCAGTCGGTAAGTTTTACTTTTCTCGCAGATCTTTTTc < 2:460050/138‑1 (MQ=255) cGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGc < 1:112101/143‑1 (MQ=255) gAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCTGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATc > 2:758749/1‑143 (MQ=255) atgTCAGACGCTTTCACGCTGGCTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGc < 1:606610/141‑1 (MQ=255) gTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGt < 1:736765/143‑1 (MQ=255) aGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTaa > 1:80273/1‑143 (MQ=255) gACGCTTCCACGCTGACTTTGATCTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAAc < 2:680959/143‑1 (MQ=255) cGCTGTCACGCTGACTGTGAACTGATCGACGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGc < 1:55406/143‑1 (MQ=255) taCGCTTACTTTGAACTGATCGAAGTTCAGGGGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATAc < 1:139391/142‑1 (MQ=255) gCTGACTTTGAACTGATCGAAGTTAAGGCGATCGCGATGATCAACATGACGCATCGCAGATTCCAGCAACGCTTTCGGTTTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCACGCGTTAAAGCCAATACGGa > 2:510252/1‑143 (MQ=255) cTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGAt < 1:375540/143‑1 (MQ=255) gACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCg < 1:838033/143‑1 (MQ=255) ttGACCTGGTCGGAGTTCAGGCGATCGAGATGACCCACATGACGCATGGCAGATTCATGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAAt < 2:817080/143‑1 (MQ=255) gAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGACCATACGGCTCGGAATgc > 2:2865/1‑142 (MQ=255) aTCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTCCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGGTCCGGCGCTAACGCCCATACGGATCGGAATGTGTTTag > 2:612740/1‑142 (MQ=255) aTCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTAt < 2:565417/143‑1 (MQ=255) aaGTTCAGGCGATCGAGAGGATCAACATGACGCAAGGAAGATACCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTACTTGCAGATCTTTTACCAGCGATCCGGCGTTAACGCCAATACGGACCGGAATGAGCTTATcgcg > 2:774985/1‑143 (MQ=255) gTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGTCTCGGAATGTTGTTGTctcgcg > 1:454276/1‑143 (MQ=255) ttCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATACGGCGCTAACGCCAAACCGGATAGGAATGTCTTTATGgcgcgc > 2:407291/1‑143 (MQ=255) aGGCGATCGAGATGATCAACATGACGCACGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGTAATGTTTTTATCGCGCGcaca < 1:198297/143‑1 (MQ=255) gCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTGACGCCGCTACGGACCGGAATGCTTTTGTCgcccgccccgt > 1:153972/1‑138 (MQ=255) gCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTGGCGCCAATACGGATCGGAATGTGTTGATCGCGCGCCCAGt > 1:41962/1‑143 (MQ=255) gATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCCATACGGATCGGAATGTTTGTATCGCGCGAAAAGTCa > 2:214072/1‑143 (MQ=255) gatgatCAACATGCCGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGCTCTGTATCCGGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAaccacc < 1:592132/143‑1 (MQ=255) tgatCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCAt < 1:655816/143‑1 (MQ=255) atCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGc > 1:63650/1‑143 (MQ=255) aTGACGCATGGCAGATCCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATAc < 1:852749/143‑1 (MQ=255) aCGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGct > 2:267711/1‑143 (MQ=255) cAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTa < 2:341894/143‑1 (MQ=255) aTTCCAGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGAGCAGTCAACGACCATGCGAATACGCTCTTCATTACCg > 2:830017/1‑143 (MQ=255) gtCCAGCAACGGCTGCGGCGTCGGTTGGCCAGACTTTTCTTGCAGAGCTTGTGCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGa < 2:142271/142‑1 (MQ=255) caccAACGCCTGCGTGCTCTGTTCGCCATTCTTTTCTTGCGGCTCTTTTTCCAGCGATCCGGCGTTACCGCCACTACGGATCGGAATGTTTTTATCGCTCGCACAGTCAACCACCCTGCGAATACGCTCTTCATTACCGatat < 2:755173/140‑1 (MQ=255) aGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTCACGCCAATACGGATTGGCATGTGCTTAGCCCGCGCACCGTCAACAACCATGCGAATACGCTCTTCATTCCCCatcta > 2:107687/1‑140 (MQ=255) aGCAACGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATAtt > 1:697731/1‑143 (MQ=255) aCGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCa < 1:365862/143‑1 (MQ=255) cGCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAg < 1:612806/143‑1 (MQ=255) gCCTGCGGCGTCGGTAAGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAgg < 1:236559/143‑1 (MQ=255) ccTGCGGCGTCGGTAAGCAATACTTTAATTGCAGATCTTGTTCCAGCGAACCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAAAGACCATGCGAATAAGCTCATAATGACCGAGACTACCAggg > 2:897776/1‑143 (MQ=255) cggcgTCGGTTCGCCATACTTTTCTTGCAGAGCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTaa < 2:651449/143‑1 (MQ=255) gTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGCTATTGCCAGGGTTAATCCg > 1:484598/1‑143 (MQ=255) cGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCa < 1:195860/143‑1 (MQ=255) | TTTTGCCAGCAAACGATAAGACTCAACAGCGAGGAAGACGTCAGACGCTTTCACGCTGACTTTGAACTGATCGAAGTTCAGGCGATCGAGATGATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCA > NC_000913/2641214‑2641491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |