Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG100.0% 166.4 / ‑6.7 55V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (27/27);  minor base A (1/0);  total (28/27)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAC  >  NC_000913/2661829‑2662101
                                                                                                                                            |                                                                                                                                    
gccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCa                                                                                                                                    <  1:327493/143‑1 (MQ=255)
 ccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAg                                                                                                                                   >  1:376831/1‑143 (MQ=255)
 ccgccCTGGCAGCCGCCTTGACCCTGACAACGGGTGGCATCTACACACTCGTGTACGGGCCTAAGTCCCTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAg                                                                                                                                   <  1:432865/143‑1 (MQ=255)
  cgccCTGGCAGCCGCCTTTTCCCTGCCACCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGa                                                                                                                                  <  1:50358/143‑1 (MQ=255)
  cgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGa                                                                                                                                  <  1:475189/143‑1 (MQ=255)
   gccCTGGCAGCGGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTGCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAc                                                                                                                                 <  1:279791/143‑1 (MQ=255)
     ccTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCa                                                                                                                               >  1:204819/1‑143 (MQ=255)
      cTGGCAGCCGCCTGTGCCCTGCTAGCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATCACTTCGCTAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAt                                                                                                                              <  2:283447/143‑1 (MQ=255)
       tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAGAGCCGACCAtt                                                                                                                             >  1:362624/1‑143 (MQ=255)
       tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtt                                                                                                                             >  1:667115/1‑143 (MQ=255)
         ggaGCCGCCTTGACCCTGAAAACGGGTGCCATCTACAGATCCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtttt                                                                                                                           <  2:684695/141‑1 (MQ=255)
           aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCGTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGAAGACCATTTCTa                                                                                                                         >  1:139083/1‑143 (MQ=255)
                         tGACACCGCGTGGCATCTACCGATTCCTCAACGGCGCTACTTACTGCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCTCCGTATTCTTGAGAGCAGACCATTTTTACGCAGACGGGaaaa                                                                                                           <  2:923031/143‑1 (MQ=255)
                         tGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTGCGCTGGAAAGCAGACCATTTTTAGGCAGACGGGaaaa                                                                                                           >  1:824241/1‑143 (MQ=255)
                         cgACAACGGGTGGCATCTCCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaaa                                                                                                           <  1:449311/142‑1 (MQ=255)
                           aCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACa                                                                                                         <  1:329720/143‑1 (MQ=255)
                           aCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACAACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACa                                                                                                         >  1:684621/1‑143 (MQ=255)
                             aaCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgt                                                                                                       >  1:719001/1‑143 (MQ=255)
                              gggggTGGGATCTAGCGATTCGTCAGCGGCGCGAACGACTGCGCCAACGGCGAGGCTGCTGGGATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAGTTTTACGCCGACGGGAAAACAgtt                                                                                                      <  2:136610/141‑1 (MQ=255)
                              aCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgtt                                                                                                      <  1:620510/143‑1 (MQ=255)
                                     gCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGAAAAGCGGCCGACTTGTACTCAGACGGGAAAACAGTTGTTCCAg                                                                                               <  1:179814/143‑1 (MQ=255)
                                      catgcaCAGATGCGGGAACGGCGGTAGTCACCGCGCCAACGGAGAGTCTGCTGGCATCTTTGCCTAACAGATAACCGCCGCGTGGTCCACGTACGCTGGAACGCAGACCATTTTCACGCAGACGGGAAAACAGTTGTTCCAGa                                                                                              <  1:716622/138‑1 (MQ=255)
                                               aTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAgg                                                                                     <  1:750172/143‑1 (MQ=255)
                                                    tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAGAt                                                                                >  1:140888/1‑143 (MQ=255)
                                                       aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGGACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTcc                                                                             >  1:492980/1‑143 (MQ=255)
                                                                    cTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGGCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaa                                                                >  2:478933/1‑143 (MQ=255)
                                                                        gCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGCAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAACGGGAAATTCCCTGACGATCGGAAatat                                                            >  1:507053/1‑143 (MQ=255)
                                                                        gCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCAACGTACGCTGGAAAGAAGAACATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATGCCCTGAGGTTCggcgcgat                                                            >  1:107048/1‑137 (MQ=255)
                                                                          cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCCTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATACGAAAGGGAAATCCCCTGCCGCTCGGAAATACCa                                                          >  2:501741/1‑143 (MQ=255)
                                                                          cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                          <  1:168976/143‑1 (MQ=255)
                                                                                 gATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACg                                                   >  1:441719/1‑143 (MQ=255)
                                                                                      tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTAcc                                              <  1:858678/143‑1 (MQ=255)
                                                                                       gctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCg                                             >  1:705719/1‑143 (MQ=255)
                                                                                         tgGTATTTTTGTCTAACATATAATCACCGCTTGGTCCACGGAAGCGGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCggg                                           <  1:325403/143‑1 (MQ=255)
                                                                                          ggCATCTGTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGc                                          <  1:10037/143‑1 (MQ=255)
                                                                                           gCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATGCCCTGACGTTCGGACATATCAGACAACGGGAACGGGca                                         >  2:269836/1‑142 (MQ=255)
                                                                                             aTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCCACGGTACCGGGCCCg                                       >  2:626495/1‑143 (MQ=255)
                                                                                              tctctcccTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCCTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCCGAAATATCAGCCAACGGTACCGGGCCCGc                                      <  1:848738/137‑1 (MQ=255)
                                                                                               cTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCt                                     >  1:279857/1‑143 (MQ=255)
                                                                                                tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGGCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                    <  1:478933/143‑1 (MQ=255)
                                                                                                tttGCCTAACAGATAACCACCGCCTGATCCACATACGCTGGAAAACAGACCATTTTTAAGCAGAAGGGAAAACAGTTGGTCCAGATAAGAAAGAGAAAAACCCTGACGTTAGGAAATATCACCCGAAGGTACGGAGCCCGcat                                    >  2:43292/1‑141 (MQ=255)
                                                                                                 ttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAAGCAGACGGGGAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGCAACATAAGCCACCGATACCGTGCCCGCtgc                                   >  1:277823/1‑141 (MQ=255)
                                                                                                    ccTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTTTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                <  1:341076/143‑1 (MQ=255)
                                                                                                     cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCCGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCagag                               >  1:378534/1‑143 (MQ=255)
                                                                                                     cTAACAGATAACCACCGCCTGGTCCACGTACGCGGGAAAGCAGAACATTTTTACACAGACGGGAGAACAGGTGTTCCAGATAAGAATGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCGCGCTTCagag                               >  1:89860/1‑143 (MQ=255)
                                                                                                      tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTGGTTCCAGATAAGAAAGGGAAATTCCCTGACCTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTGagagc                              >  2:333268/1‑142 (MQ=255)
                                                                                                       aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGGAAACAGTTGTTACAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTCCAGCGtt                             >  2:316919/1‑143 (MQ=255)
                                                                                                         ccgATAACCACCGCGTGGTCCACGTACGCTGGAAAGCAGACCGTGTGTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                           <  1:94279/141‑1 (MQ=255)
                                                                                                           taTAACCTCCGCCTGATCCACGTACGCTGGAAAGCAGACCATTTTTAAGCAGTCAGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                         <  2:107725/142‑1 (MQ=255)
                                                                                                            aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCCGCCAACGGTACCGGGACCGCTTCAGAGTTGAgcg                        >  2:624865/1‑143 (MQ=255)
                                                                                                            aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGGTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCAGGCACGCTGCAGAGGTGAgcg                        >  1:745961/1‑143 (MQ=255)
                                                                                                                      ccTGGTCGACGGACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAg              <  2:893313/143‑1 (MQ=255)
                                                                                                                             cACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCTGCCAACGGTACCGGGCCCGCTTCAGAGTTGCGCGCAACGTCAAGCATTGCg       <  2:490742/143‑1 (MQ=255)
                                                                                                                                  ttgCTGGAAAGCAGGCCACTTTTACGCAGACGGGAAAACAGTTGCTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc  <  1:883482/141‑1 (MQ=255)
                                                                                                                                  aCGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAGGCATTGCGGTCAc  >  1:525267/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                    
GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAC  >  NC_000913/2661829‑2662101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑