Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,041,632 T→A 100% V107E (GTG→GAG)  ygfZ → folate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,6320TA97.9% 134.6 / ‑5.8 50V107E (GTG→GAG) ygfZfolate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (23/25);  minor base C (2/0);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041492‑3041767
                                                                                                                                            |                                                                                                                                       
cgcgCATGGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAgaga                                                                                                                                       <  2:840103/143‑1 (MQ=255)
        gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgcg                                                                                                                               <  1:659498/143‑1 (MQ=255)
          gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGGCTGAACTGAAAAAATATGCGGTATTCTCTCAAGCGACCATCGcccc                                                                                                                             >  1:737145/1‑143 (MQ=255)
          gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                             <  2:831725/143‑1 (MQ=255)
             gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGCGACCATCGCGCCAga                                                                                                                          >  1:631414/1‑143 (MQ=255)
                      aaaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGATTCTGCCAACCGCAGCTGACTGAACTGAAAAAATATGCAGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCg                                                                                                                 >  1:352130/1‑143 (MQ=255)
                         aTGTGGAGCAATTTACGTCTGTTCCCCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtgt                                                                                                              <  1:250720/143‑1 (MQ=255)
                              gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGCCCATCGCGCCAGACGACGAGCGTGtgctgc                                                                                                         >  1:221572/1‑143 (MQ=255)
                                 cgaTTTGCGTCTGTTCCGCTACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTTCAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                      <  2:118713/141‑1 (MQ=255)
                                   acttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACAAGCGTGTGCTGCTTGgt                                                                                                    <  1:169513/141‑1 (MQ=255)
                                      taggTCTGTTCCGTGACGGCGATGGCTTTGCATGGATTGAAAGGCGCAGCGTGCGTGAACCGCAGCTGCCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt                                                                                                 <  1:567999/140‑1 (MQ=255)
                                            tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTGGCGGGt                                                                                           >  1:723873/1‑143 (MQ=255)
                                              ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt                                                                                         <  1:166237/143‑1 (MQ=255)
                                               tCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtttt                                                                                        <  1:630017/143‑1 (MQ=255)
                                                ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                       >  1:806186/1‑143 (MQ=255)
                                                               cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGcgcgccgcgc                                                                        >  1:918083/1‑143 (MQ=255)
                                                                tttGCACGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt                                                                       <  1:18757/143‑1 (MQ=255)
                                                                   gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTAATCTCTAAAGAGACCATCGCCCAAGACGACGAGCGTGTGCGGCTTGGTGGTGCCGGTGTTAAGGCGcgcgacgccgcggt                                                                    >  2:683699/1‑137 (MQ=255)
                                                                    cATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                                                                   >  1:370505/1‑143 (MQ=255)
                                                                     aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAGTATGCGGTATTCTCTAAAGAGACCATAGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCGGGGTTTCAGGCGAGCGCAGCGCGCGCaa                                                                  >  2:638700/1‑143 (MQ=255)
                                                                     aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcggggcgcgccagcac                                                                  >  2:143665/1‑137 (MQ=255)
                                                                         aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGGGGCAGGCGCGCGCCGCGCTGGCAAAtct                                                              >  1:889250/1‑143 (MQ=255)
                                                                         aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGCTGAATACGTTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                              >  1:889644/1‑143 (MQ=255)
                                                                           tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                            <  1:452988/143‑1 (MQ=255)
                                                                            gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGACTTCAGGCGCGCGCCGCGCTGGCAAATctctc                                                           >  2:943268/1‑142 (MQ=255)
                                                                             aaCGGCGCAGCGTGCGTGAACGGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                          <  2:139049/143‑1 (MQ=255)
                                                                              aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCTGTGGTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                         >  1:637874/1‑143 (MQ=255)
                                                                                       cgtgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGTGCCAGACGACGAGCGCGTGCTGCTTGGTGTTGCAGGTTTTCAGACGCGCGCCGCGCTGGCAACTCTAGTTCGCGCACttc                                                >  2:806754/1‑141 (MQ=255)
                                                                                           tttgAACTTCAGCTGTCTTTTCTGAAAAAATATGAGGGACTCGCGAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCTGGCGTGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc                                            <  1:180013/141‑1 (MQ=255)
                                                                                            gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCTCGCTGGCAACACTCTTTAGCAAAATGctgtag                                           >  1:77793/1‑138 (MQ=255)
                                                                                             tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCCTCgc                                          >  1:398788/1‑142 (MQ=255)
                                                                                              gacccGCAGCTCACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGAGACCACCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa                                         <  1:167619/140‑1 (MQ=255)
                                                                                              gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCCCTTTAGCGAACTGCCTTCgaa                                         >  2:572821/1‑143 (MQ=255)
                                                                                               aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                        <  2:379616/143‑1 (MQ=255)
                                                                                                    cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGCCGACGAGCTTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaa                                   <  1:938680/143‑1 (MQ=255)
                                                                                                    cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGACGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAGTGCCTCGgaaagaaac                                   >  2:62727/1‑142 (MQ=255)
                                                                                                        tgactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                               <  1:463899/143‑1 (MQ=255)
                                                                                                        tgactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                               <  1:463761/143‑1 (MQ=255)
                                                                                                         gactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGCAAAACAgg                              >  1:373587/1‑143 (MQ=255)
                                                                                                         gactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGCGTTGCCGGCTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGACAGACAAAccag                              >  2:248657/1‑140 (MQ=255)
                                                                                                         gacggaACGGAAAAAATGTGAGGTATTCTCGAAAGAGACCATCGCGCAAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                              <  2:117801/143‑1 (MQ=255)
                                                                                                          actgaactgaAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGGGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGGCTTCGAAAGAAAAACAGGt                             >  1:189350/1‑143 (MQ=255)
                                                                                                           gtgagctgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGGGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                            <  1:939333/142‑1 (MQ=255)
                                                                                                           ctgaactgaaAAAATATGCGATATTTTCTATTGATTCTAATTCGCGTTTTGTCGAGCGTGTCCTGCTTATTTTTTCTGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                            <  1:210791/143‑1 (MQ=255)
                                                                                                             gaactggaAAGATATGCGGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGt                          <  2:837708/143‑1 (MQ=255)
                                                                                                                        atagGCGGGATTCTCTACAGAGCCGATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTGGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcg               <  1:919779/143‑1 (MQ=255)
                                                                                                                         tatGCGGTATTCGCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcgc              <  2:347452/143‑1 (MQ=255)
                                                                                                                            gCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAc           <  1:860052/143‑1 (MQ=255)
                                                                                                                                 aTTCTCTACAGAGTCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt      <  1:507805/143‑1 (MQ=255)
                                                                                                                                     tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCATCGAAAGAAAAACAGGTAGTCAAAGAACGCGCGACCACCTTGcc  >  2:29561/1‑142 (MQ=255)
                                                                                                                                     tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATATATATATCGAACTGCCTTCGACAGAAAAACCGGTAGTCAAAGAAGGCGCGACCACTTTGCt  >  1:215465/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                       
CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041492‑3041767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑