Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 371,067 T→A 9.7% I264N (ATT→AAT)  mhpB → 3‑carboxyethylcatechol 2,3‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913371,0670TA 9.7% 127.5 / 4.5 51I264N (ATT→AAT) mhpB3‑carboxyethylcatechol 2,3‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (19/26);  new base A (3/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

CGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTAAGC  >  NC_000913/370930‑371203
                                                                                                                                         |                                                                                                                                        
cgccgcTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCg                                                                                                                                     <  1:797185/143‑1 (MQ=255)
   cgcTGAGGAGTTTGTTGGGGATCACAGAACGCTGCATCCGCTCAACCCGGTTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGaa                                                                                                                                  <  1:725602/143‑1 (MQ=255)
   cgcTGAGACGGTTGTTTAGGAGCAGAGAGCGCTGCATCCGCTCGACCCGATTTGGGATAACCAGTTCATGACTGTGCTGGAGCAGGGACGCATACAGGAACTGGATGACGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGaa                                                                                                                                  <  2:424703/143‑1 (MQ=255)
    gcTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGaaa                                                                                                                                 <  1:597213/143‑1 (MQ=255)
      tGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCCGTAACGAAGAGCTTTCCGGCATTGCCGGAAAGt                                                                                                                               >  1:249444/1‑143 (MQ=255)
      gggggaGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTGCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGACGCCGTCAGTAACGAAGAGCTTTCCGGCAATGGCGGAAAgc                                                                                                                               <  2:427105/138‑2 (MQ=255)
            gTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGacac                                                                                                                         >  1:266856/1‑143 (MQ=255)
              gtgttgAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACAt                                                                                                                       <  1:65309/142‑1 (MQ=255)
                gttgAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGa                                                                                                                     <  1:800334/143‑1 (MQ=255)
                        tCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCaaaa                                                                                                             <  1:305261/143‑1 (MQ=255)
                          agagAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACCCATGCAATCACAAcc                                                                                                           >  1:240550/1‑143 (MQ=255)
                           gagaACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAACAccc                                                                                                          >  2:479764/1‑142 (MQ=255)
                              aaCGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGTCAGTGCGGGAAAGTCGACACATGAAATCAAAACCTggg                                                                                                       >  1:638556/1‑143 (MQ=255)
                                 gCTGCATCCGCTCAACCCGATCGGGGATAACCAGTTCAGGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTcg                                                                                                    <  2:798209/143‑1 (MQ=255)
                                      aTCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCt                                                                                               >  1:309669/1‑143 (MQ=255)
                                           cTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGc                                                                                          >  1:139456/1‑143 (MQ=255)
                                           cTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGc                                                                                          >  1:747616/1‑143 (MQ=255)
                                                    aTTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTc                                                                                 <  1:369970/143‑1 (MQ=255)
                                                      ttGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGATGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTg                                                                               <  2:240577/143‑1 (MQ=255)
                                                            tAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTg                                                                         <  1:41965/143‑1 (MQ=255)
                                                            tAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGACAATAATGGGAAGTCGACTCATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTg                                                                         >  1:433672/1‑143 (MQ=255)
                                                            catccAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGCAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTCCTGCGTTTg                                                                         >  2:413891/4‑143 (MQ=255)
                                                            catccAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAAGCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTg                                                                         >  2:413830/4‑143 (MQ=255)
                                                             agccAGTTCGTGACTTTGCTGGCTCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCCGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTgg                                                                        <  2:747870/141‑1 (MQ=255)
                                                             aaCCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTgg                                                                        <  1:403553/143‑1 (MQ=255)
                                                               ccAGTTCATGGCTGTGCTGGAGGAGGGACGCATACAGGGACTGGGTGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCa                                                                      <  2:865886/143‑1 (MQ=255)
                                                               ccAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTCTTTCTGCGTTTGGCa                                                                      >  2:645652/1‑143 (MQ=255)
                                                                cAGTTCATGACTTTGCTGGAGCAGGGCCGCACACAGGAACTGGAGGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCaa                                                                     <  2:449409/143‑1 (MQ=255)
                                                                        gACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGCGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTa                                                             >  2:704736/1‑143 (MQ=255)
                                                                          cTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTGCTGCGGTTGGCAACTTGCGTAGc                                                           >  1:411360/1‑143 (MQ=255)
                                                                           tttGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTCTTGCCGCTCTCTCTGCGTTTGGCGACTGACggcgcg                                                          >  2:525016/1‑138 (MQ=255)
                                                                               cgggAGCAGGGACGCATACACGGACTGTCCGCCGTCAGGAACGAAGCGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAGCCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAgg                                                      <  2:149494/141‑1 (MQ=255)
                                                                                    gCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGtt                                                 <  1:356954/143‑1 (MQ=255)
                                                                                            gCATCCACGAACTGGATGCCTTCAGTAACGCAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGc                                         <  1:449482/143‑1 (MQ=255)
                                                                                            gCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCACTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGc                                         <  1:900792/143‑1 (MQ=255)
                                                                                               tACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGGCGACACAGGAAATCAAAACCTGAGTCGCCGCGTTTGCCGCTATTCCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTGATTACCGCCCa                                      >  2:873496/1‑143 (MQ=255)
                                                                                                 cAGGAACTGGATGCCGTCAGTAACGAAGAGCGTTCCGCCATTGCCGGATAGTCGACACATGAAATCAAGACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGGTAATAACGcccttc                                    <  2:129220/143‑4 (MQ=255)
                                                                                                  aGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGGCAATGGCGGAAAgc                                                                                                                               <  2:123999/51‑2 (MQ=21)
                                                                                                  aGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGGCAATGGCGGAAAgc                                                                                                                               >  1:123999/1‑50 (MQ=21)
                                                                                                    gAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTCATGCAGTGTTTTCGTCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATccc                                 >  1:214052/1‑143 (MQ=255)
                                                                                                     aaCTGCATGCCGTCACTAACGAAGAGCTTTCCGCCTTTGCCGGATAGTCCACACATGAAATCTAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCg                                <  2:744262/143‑1 (MQ=255)
                                                                                                            tGCCGTCAGTAACGAAGAGGTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGa                         <  1:802457/143‑1 (MQ=255)
                                                                                                             gCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCATTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGAt                        <  1:482986/143‑1 (MQ=255)
                                                                                                               cGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTg                      >  1:485145/1‑143 (MQ=255)
                                                                                                                 ttaGTAACGAAGATCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCGGCGTTTGCCGCTATTCCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGcc                    <  1:43277/141‑1 (MQ=255)
                                                                                                                 tCAGTAACGAAGAGCTGTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGcc                    <  1:479764/143‑1 (MQ=255)
                                                                                                                     tAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCCACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGCGGAGTGACGGAt                >  1:231831/1‑143 (MQ=255)
                                                                                                                               cTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTCATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGtt      >  1:614130/1‑143 (MQ=255)
                                                                                                                                tttCCGGCAATGGCGGAAAGTCGACACATGAAATCAAAACCTGGGTTGCCGCTTTTGCCGCTAGTTCTGCGTTTGGCAACTGGCGTAGCGACGGGCGTTATTACCGCCCAATCCCGGACTCGATTACGGGCTTTGGGTCGtca     >  2:5961/1‑141 (MQ=255)
                                                                                                                                tttCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTa     >  1:219404/1‑143 (MQ=255)
                                                                                                                                   ccGCCATTGCCTGAAAGTCGACACATGTAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGTGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTAAgc  <  1:613318/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTAAGC  >  NC_000913/370930‑371203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑