Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,259,122 T→G 8.6% E176D (GAA→GAC) ‡ tdcG ← L‑serine deaminase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,259,1220TG 8.6% 103.3 / 4.5 47E176D (GAA→GAC) ‡tdcGL‑serine deaminase III
Reads supporting (aligned to +/- strand):  ref base T (21/22);  new base G (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-02

CCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAG  >  NC_000913/3258981‑3259261
                                                                                                                                             |                                                                                                                                           
cccACGTTCAATACCGTCATGCATCACTTGCCACGTTCGTGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGtt                                                                                                                                            <  2:661325/143‑1 (MQ=255)
   aCGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCAc                                                                                                                                         <  1:231409/143‑1 (MQ=255)
    cGTTCGATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCAcc                                                                                                                                        <  2:692216/143‑1 (MQ=255)
          ctcccGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTATGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGa                                                                                                                                  <  2:207703/140‑1 (MQ=255)
          aTACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGGTGAACTGCTGa                                                                                                                                  <  1:502984/143‑1 (MQ=255)
           tACCGTCATGCATCACTTGCCAGATACTGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAg                                                                                                                                 >  2:684692/1‑143 (MQ=255)
                tCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGGTGAACTGCTGaaaatgt                                                                                                                            <  2:599132/143‑7 (MQ=255)
                  aTGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCCCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGCTGTAATCCCACATTTTCAGCCGTTCACCAGCTCAGTGTAAAt                                                                                                                          >  2:799089/1‑143 (MQ=255)
                    gCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCg                                                                                                                        >  1:804583/1‑143 (MQ=255)
                       tCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTaa                                                                                                                     <  1:233768/143‑1 (MQ=255)
                       tCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTaa                                                                                                                     >  1:654721/1‑143 (MQ=255)
                            tGCCATATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTAc                                                                                                                <  2:833612/143‑1 (MQ=255)
                                     cGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGTCCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTc                                                                                                       <  1:82918/143‑1 (MQ=255)
                                      gggCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGAACAGCGGAAAGTAGGGACGTGTCg                                                                                                      >  2:353747/1‑143 (MQ=255)
                                       ggCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGa                                                                                                     <  2:407313/143‑1 (MQ=255)
                                        gCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCGTCAGACCAGATATAGACAGGCCGTTGTAAGCACACATTTTCAGCAGTTCACCTGCTGAGTGGCAATCGTCAGGTACGGACGTTTCggc                                                                                                    >  1:421067/1‑141 (MQ=255)
                                             aCCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTGCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGt                                                                                               <  1:332162/143‑1 (MQ=255)
                                               ctgcctCAATTTCCGCTTTGCGTCGCAGCGCTAGCTCGTTGTGCATCATCCGACCAGATATAGATAGGCCGTTGTAATCACTCATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGc                                                                                             <  2:359994/138‑1 (MQ=255)
                                               cgtggTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTATGCATCAGCAGACCAGATATAGACAGGCAGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGc                                                                                             <  2:813356/139‑1 (MQ=255)
                                                     cAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGATCAGAGATTTACAGGCCGTTGTAATCACACAGTTTCAGCAGTTAAAGTCCTTAGTGGAAATCGTAAGGTACGGCCGTCTCGACCACGTGCGACCgg                                                                                       >  2:371626/1‑143 (MQ=255)
                                                       aTTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGCCATCGTGCGACAgggc                                                                                     >  2:932412/1‑141 (MQ=255)
                                                        tttCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCg                                                                                    <  1:508405/143‑1 (MQ=255)
                                                             gCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACCGGCCGCTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTAAGATCGTTTCGACATCGTGCGGCCTGCCGAAgtg                                                                               >  2:363607/1‑143 (MQ=255)
                                                              cTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACGTAGTGCGCCAGGCCGAAgtgt                                                                              >  1:24162/1‑143 (MQ=255)
                                                                    tgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttct                                                                        >  1:847785/1‑143 (MQ=255)
                                                                    tgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttct                                                                        >  1:904249/1‑143 (MQ=255)
                                                                     gcgcAGCGCTAGCTCGTTGTGCATCATCAGACCAGATACAGACAGGCCGTGGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttctt                                                                       >  1:398092/1‑143 (MQ=255)
                                                                            gcTAGCCCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTACTCACACATTTTCAGCAGTTCACCCGCTGAGTGGAAATCTTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAAt                                                                <  1:96857/143‑1 (MQ=255)
                                                                               ggCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATaaa                                                             <  2:219648/142‑1 (MQ=255)
                                                                                     ttGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACccgcc                                                       >  1:78955/1‑143 (MQ=255)
                                                                                        ggCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACccgccgcc                                                    <  1:697493/142‑1 (MQ=255)
                                                                                               tcaGACCAGATATAGTCAGGCCGTTGTACTCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGGGCGGCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACagag                                             >  1:82745/1‑143 (MQ=255)
                                                                                                  gACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACCATAAACCCGCCGCCGACAGAGtca                                          >  1:452018/1‑141 (MQ=255)
                                                                                                   aCCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTACGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACCGAGTAAt                                         >  1:750458/1‑143 (MQ=255)
                                                                                                         gtgtgACAGGCGGTTGTAACCACGCATTTTCAGCAGTTCACCTGGTGGGTGGAAATCGTGAGCTACGGACGTTTCGCCATCGTGCGACAGGCTGAAGTGTTCTTCTTCTACAATAAACCCGCCGCCGACAGAGTAATAGGttt                                   <  2:940348/139‑1 (MQ=255)
                                                                                                              aCAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                              <  1:251228/143‑1 (MQ=255)
                                                                                                                 ggCCGTTGTAATCCCACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAAt                           <  1:495373/143‑1 (MQ=255)
                                                                                                                  gCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCAGCAGAGGAAACGGTTTTACTTACTa                          >  2:228696/1‑143 (MQ=255)
                                                                                                                            tCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTGATAGGTTTTGCTTGCTAGCTCTTCCTg                >  1:14552/1‑143 (MQ=255)
                                                                                                                             cacacaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGt               <  1:377751/143‑1 (MQ=255)
                                                                                                                                 caTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTATTCctctctct           >  2:882669/1‑143 (MQ=255)
                                                                                                                                   cactCAGCAGGTGAACTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCCCTTCGTGTCCCTTc         >  2:166884/4‑143 (MQ=255)
                                                                                                                                    actCAGCAGGTGAACTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAAGAATAGGGTTTACTAAATAGCTCTTCCTGTCCGTTcc        >  2:830202/3‑143 (MQ=255)
                                                                                                                                     ttCAGCCGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa       <  1:679196/143‑1 (MQ=255)
                                                                                                                                      tCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCCACAATACCCCCGCCCCCGACAGAATAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAg      <  1:18159/143‑1 (MQ=255)
                                                                                                                                       cagcagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAgg     <  1:166542/143‑1 (MQ=255)
                                                                                                                                         gcagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCa   <  1:313492/143‑1 (MQ=255)
                                                                                                                                          cagTTCACCTGCTGAGTGGAAATCGTAAGGTAAGGCAGTATCTACACCCTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTGATAGGTGTTCCTTAATAACTCTTCCTGTCCCTTCCACGCAg  >  2:671297/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                           
CCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAG  >  NC_000913/3258981‑3259261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑