Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,259,124 C→G 8.1% E176Q (GAA→CAA) ‡ tdcG ← L‑serine deaminase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,259,1240CG 8.1% 118.5 / 2.5 47E176Q (GAA→CAA) ‡tdcGL‑serine deaminase III
Reads supporting (aligned to +/- strand):  ref base C (20/22);  new base G (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

ACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAT  >  NC_000913/3258984‑3259265
                                                                                                                                            |                                                                                                                                             
aCGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCAc                                                                                                                                             <  1:231409/143‑1 (MQ=255)
 cGTTCGATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCAcc                                                                                                                                            <  2:692216/143‑1 (MQ=255)
       ctcccGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTATGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGa                                                                                                                                      <  2:207703/140‑1 (MQ=255)
       aTACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGGTGAACTGCTGa                                                                                                                                      <  1:502984/143‑1 (MQ=255)
        tACCGTCATGCATCACTTGCCAGATACTGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAg                                                                                                                                     >  2:684692/1‑143 (MQ=255)
             tCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGGTGAACTGCTGaaaatgt                                                                                                                                <  2:599132/143‑7 (MQ=255)
               aTGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCCCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGCTGTAATCCCACATTTTCAGCCGTTCACCAGCTCAGTGTAAAt                                                                                                                              >  2:799089/1‑143 (MQ=255)
                 gCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCg                                                                                                                            >  1:804583/1‑143 (MQ=255)
                    tCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTaa                                                                                                                         >  1:654721/1‑143 (MQ=255)
                    tCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTaa                                                                                                                         <  1:233768/143‑1 (MQ=255)
                         tGCCATATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTAc                                                                                                                    <  2:833612/143‑1 (MQ=255)
                                  cGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGTCCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTc                                                                                                           <  1:82918/143‑1 (MQ=255)
                                   gggCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGAACAGCGGAAAGTAGGGACGTGTCg                                                                                                          >  2:353747/1‑143 (MQ=255)
                                    ggCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGa                                                                                                         <  2:407313/143‑1 (MQ=255)
                                     gCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCGTCAGACCAGATATAGACAGGCCGTTGTAAGCACACATTTTCAGCAGTTCACCTGCTGAGTGGCAATCGTCAGGTACGGACGTTTCggc                                                                                                        >  1:421067/1‑141 (MQ=255)
                                          aCCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTGCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGt                                                                                                   <  1:332162/143‑1 (MQ=255)
                                            ctgcctCAATTTCCGCTTTGCGTCGCAGCGCTAGCTCGTTGTGCATCATCCGACCAGATATAGATAGGCCGTTGTAATCACTCATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGc                                                                                                 <  2:359994/138‑1 (MQ=255)
                                            cgtggTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTATGCATCAGCAGACCAGATATAGACAGGCAGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGc                                                                                                 <  2:813356/139‑1 (MQ=255)
                                                  cAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGATCAGAGATTTACAGGCCGTTGTAATCACACAGTTTCAGCAGTTAAAGTCCTTAGTGGAAATCGTAAGGTACGGCCGTCTCGACCACGTGCGACCgg                                                                                           >  2:371626/1‑143 (MQ=255)
                                                    aTTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGCCATCGTGCGACAgggc                                                                                         >  2:932412/1‑141 (MQ=255)
                                                     tttCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCg                                                                                        <  1:508405/143‑1 (MQ=255)
                                                          gCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACCGGCCGCTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTAAGATCGTTTCGACATCGTGCGGCCTGCCGAAgtg                                                                                   >  2:363607/1‑143 (MQ=255)
                                                           cTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACGTAGTGCGCCAGGCCGAAgtgt                                                                                  >  1:24162/1‑143 (MQ=255)
                                                                 tgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttct                                                                            >  1:847785/1‑143 (MQ=255)
                                                                 tgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttct                                                                            >  1:904249/1‑143 (MQ=255)
                                                                  gcgcAGCGCTAGCTCGTTGTGCATCATCAGACCAGATACAGACAGGCCGTGGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttctt                                                                           >  1:398092/1‑143 (MQ=255)
                                                                         gcTAGCCCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTACTCACACATTTTCAGCAGTTCACCCGCTGAGTGGAAATCTTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAAt                                                                    <  1:96857/143‑1 (MQ=255)
                                                                            ggCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATaaa                                                                 <  2:219648/142‑1 (MQ=255)
                                                                                  ttGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACccgcc                                                           >  1:78955/1‑143 (MQ=255)
                                                                                     ggCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACccgccgcc                                                        <  1:697493/142‑1 (MQ=255)
                                                                                            tcaGACCAGATATAGTCAGGCCGTTGTACTCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGGGCGGCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACagag                                                 >  1:82745/1‑143 (MQ=255)
                                                                                               gACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACCATAAACCCGCCGCCGACAGAGtca                                              >  1:452018/1‑141 (MQ=255)
                                                                                                aCCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTACGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACCGAGTAAt                                             >  1:750458/1‑143 (MQ=255)
                                                                                                      gtgtgACAGGCGGTTGTAACCACGCATTTTCAGCAGTTCACCTGGTGGGTGGAAATCGTGAGCTACGGACGTTTCGCCATCGTGCGACAGGCTGAAGTGTTCTTCTTCTACAATAAACCCGCCGCCGACAGAGTAATAGGttt                                       <  2:940348/139‑1 (MQ=255)
                                                                                                           aCAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                  <  1:251228/143‑1 (MQ=255)
                                                                                                              ggCCGTTGTAATCCCACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAAt                               <  1:495373/143‑1 (MQ=255)
                                                                                                               gCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCAGCAGAGGAAACGGTTTTACTTACTa                              >  2:228696/1‑143 (MQ=255)
                                                                                                                         tCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTGATAGGTTTTGCTTGCTAGCTCTTCCTg                    >  1:14552/1‑143 (MQ=255)
                                                                                                                          cacacaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGt                   <  1:377751/143‑1 (MQ=255)
                                                                                                                              caTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTATTCctctctct               >  2:882669/1‑143 (MQ=255)
                                                                                                                                cactCAGCAGGTGAACTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCCCTTCGTGTCCCTTc             >  2:166884/4‑143 (MQ=255)
                                                                                                                                 actCAGCAGGTGAACTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAAGAATAGGGTTTACTAAATAGCTCTTCCTGTCCGTTcc            >  2:830202/3‑143 (MQ=255)
                                                                                                                                  ttCAGCCGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa           <  1:679196/143‑1 (MQ=255)
                                                                                                                                   tCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCCACAATACCCCCGCCCCCGACAGAATAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAg          <  1:18159/143‑1 (MQ=255)
                                                                                                                                    cagcagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAgg         <  1:166542/143‑1 (MQ=255)
                                                                                                                                      gcagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCa       <  1:313492/143‑1 (MQ=255)
                                                                                                                                       cagTTCACCTGCTGAGTGGAAATCGTAAGGTAAGGCAGTATCTACACCCTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTGATAGGTGTTCCTTAATAACTCTTCCTGTCCCTTCCACGCAg      >  2:671297/1‑143 (MQ=255)
                                                                                                                                           gcACCGGCGGAGTGGAAAGCGTGAGGTACGGACGTGTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAt  <  1:146659/142‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
ACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAT  >  NC_000913/3258984‑3259265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑