| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,856,594 | A→C | 10.6% | K60N (AAA→AAC) | yidI → | putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,856,594 | 0 | A | C | 10.6% | 76.9 / 4.2 | 38 | K60N (AAA→AAC) | yidI | putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (21/13); new base C (2/2); total (23/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01 | |||||||||||
TGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATT > NC_000913/3856455‑3856735 | tGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGACTAAAAGGc > 1:725286/1‑143 (MQ=255) ggTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAAt < 1:65907/143‑1 (MQ=255) ggTGCCATCGCTTTAATGATGGGGATTATCCACTTTCCATTCAGCCCTTTCTCCGCCCCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGCGGTCTTCTCGCCGGAATAAAATGCGTAAAAAt > 1:609757/1‑143 (MQ=255) gTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAAtt < 2:499092/143‑1 (MQ=255) tGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAACGCCTCGTGGCGGACAACACGGCTGAAATTAAAAGAGGTCGTCTCGCCGGCATCAAAGGCGAAAAAAGTa > 2:783717/1‑143 (MQ=255) ccATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTacc > 2:279645/1‑143 (MQ=255) aTCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGGCAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTaccaa > 1:233647/1‑142 (MQ=255) tCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTTAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTaccacc > 1:502588/1‑143 (MQ=255) gatgGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGaaaa < 1:753255/143‑1 (MQ=255) gATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAACGGCGAAAAAATTACCACCGTGGAGAAAAAAGAgg > 2:738939/1‑143 (MQ=255) gATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAACGGCGAAAAAATTACCACCGTGGAGAAAAAAGAgg > 2:262204/1‑143 (MQ=255) gATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAgg > 2:750201/1‑143 (MQ=255) gATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATGAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTCGAGGAAAAAGCgg > 2:141244/1‑143 (MQ=255) tCCACTTTTCATTCGGCCCGTGGTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATGAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTa < 2:134540/143‑1 (MQ=255) ccACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAAGGTTTGAAAGCGTCGTGGCGGACAAGACGGCTAGATGTAGAAGAGGTCTTCTCGCCGGAATAACAGGCGAACAGAGTACCACCGTGGAGAGGAGAGAGGACGGAg > 2:455009/1‑143 (MQ=255) tCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGa > 1:861791/1‑143 (MQ=255) ggCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAAt > 1:310461/1‑143 (MQ=255) ttCTCCTCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCtcaa < 1:627323/143‑1 (MQ=255) tcCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCtcaatca > 2:123221/1‑143 (MQ=255) cGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCaaa > 1:661349/1‑143 (MQ=255) accaccGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAACGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCg < 1:262204/143‑1 (MQ=255) accaccGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAACGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCg < 1:738939/143‑1 (MQ=255) caccGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGGGGAGAAACAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGt > 2:788485/1‑143 (MQ=255) gCCAACGTTTGAAAGCATCGTGGCGGACAAAAAGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGAAAAAATCCTCAATCAAAGCGGTATCg > 2:274579/1‑143 (MQ=255) aaaGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCAtt < 1:241937/143‑1 (MQ=255) cATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATtgctg < 1:279645/143‑1 (MQ=255) aTCGTGGCGCACTGACGGGCTGTAATTAAAAGAGGTCGTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATtgctgc < 1:836276/143‑1 (MQ=255) aaCGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCAt > 1:330966/1‑143 (MQ=255) cGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAGAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCAttt > 1:36048/1‑143 (MQ=255) aTTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGGATTGATTGGAgg > 2:1474/1‑143 (MQ=255) ttAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGt > 1:173500/1‑143 (MQ=255) tAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTa > 1:526328/1‑143 (MQ=255) agggcgtctcGTCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTa < 1:580449/137‑1 (MQ=255) agGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAACTCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTGTTGGCGGTGTGCgga > 2:944575/1‑141 (MQ=255) ggTCTGCTCGCCGGAATAAAAGGCCAAAAAAGTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAACTCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTaa < 1:1474/143‑1 (MQ=255) gtgAATAAAAGGCGAAAAAATTACCAACGTGGAGTAGAAAGAGGACGTAGATGTCGAGACAATCCTCAATCAAGGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCt < 1:23610/141‑1 (MQ=255) ggAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTg < 1:85886/143‑1 (MQ=255) aTAAAAGCCGAACAAACCACCCCCGTTGACAAAAAAGCGGACGTAGATGTCGATAAAACCCTCAATCAAAGCGGTATCGCACTGGTCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTgggg < 1:68658/143‑1 (MQ=255) aaaGGCGAAAAAATTACCACCGTGGAGAAAAACGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGGAt > 1:39838/1‑143 (MQ=255) aaaGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGAAGTCGATAAAATCCTCAATCAAAGCGGTATAGCACTGGCCATTGCTGCGCTACTTCGCGCATTTATTGGAGGTATGCGTGAGGAAAATGGCTGGGGCac > 2:140513/1‑142 (MQ=255) aaGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCAtt > 1:395895/1‑143 (MQ=255) | TGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATT > NC_000913/3856455‑3856735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |