| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,296,060 | C→T | 29.8% | intergenic (+266/+376) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 29.8% | 62.0 / 36.3 | 62 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base C (32/11); new base T (9/9); total (42/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCT > NC_000913/4295918‑4296198 | tgatccGTCTATGCCGCACCCTGCATCATCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCCTCCGACATCAAc < 1:220528/138‑1 (MQ=11) ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCGATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGcc > 1:894681/1‑143 (MQ=17) ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGcc > 1:849390/1‑143 (MQ=32) gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCt > 1:545418/1‑143 (MQ=32) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATTCGCCg > 1:804920/1‑143 (MQ=17) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGCGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACg > 2:564279/1‑143 (MQ=17) cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCtt < 1:656826/143‑1 (MQ=32) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTT‑ACGCCGCATCAGACCTCCAGAGCTGAAGCGACGCTTGc > 2:446065/1‑143 (MQ=11) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACCTCCGCGCCTGCGCCGACGCTCGc > 1:124359/1‑143 (MQ=11) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc > 1:907029/1‑143 (MQ=21) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTTGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCCTCCGACATCAACGCCTGATGCGACGCttcctgc > 1:381365/1‑138 (MQ=255) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc > 1:98178/1‑143 (MQ=25) cATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 2:34480/143‑1 (MQ=38) aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt < 1:177229/143‑1 (MQ=39) gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACCTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa > 1:838317/1‑143 (MQ=25) gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:628717/143‑1 (MQ=21) gCCTGATAAGACGCGTTAAGCGTCGCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa > 2:355017/1‑143 (MQ=11) ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:887122/143‑1 (MQ=39) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg > 2:655999/1‑143 (MQ=25) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCGTGGTTTAGCGTGATTGTGTAGGTCGGATAAGGCGTTT‑ACGCTGCATCCGACATCAACGCCTGATGAGAAGCTTGCCGCGTCTTATCAGGc > 2:185496/1‑143 (MQ=11) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 1:658476/1‑143 (MQ=25) aTGCGAGGCTTGTCGCGCCTTAGCAGGCGTACGCCAGACAGCGCAATAGCCTGTTTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt < 2:849390/143‑1 (MQ=11) tGCTACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa > 2:880561/1‑143 (MQ=21) gCGACGCTCACCGCGTCTTATCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:163026/143‑2 (MQ=255) gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc > 1:19157/1‑143 (MQ=37) gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCGTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg > 2:620304/1‑143 (MQ=37) cgcgTCTTATGCGGCCGACGCCAGACAGCGCAATGGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGTCTACGCCAGACAgcg < 2:796208/143‑1 (MQ=14) gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAacgc > 2:259313/1‑143 (MQ=255) gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc > 2:260326/1‑143 (MQ=37) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGc < 2:877283/91‑1 (MQ=21) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGc > 1:877283/1‑91 (MQ=33) tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCGGCGCTCTAGctt > 2:687887/1‑141 (MQ=11) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTgcgttc > 2:453362/1‑138 (MQ=12) tctgcagccagcGCACTTGCTGGCTGTAGCGTGATTTTGTAGGGCGGATAAGGCGTTT‑CCGCCGTATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc < 2:334298/139‑1 (MQ=14) aCGTCAGATAGCGCAGTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc < 2:593369/143‑1 (MQ=255) cGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg < 1:240741/143‑1 (MQ=38) cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCCTTTCACG‑CTCATCCGGTATCAACGCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCGGCGCTATAGCCTGCTATAGCGTgc > 2:417236/1‑142 (MQ=11) cacAATAGTCTGAGGTGGCGTCATTGTGTGGGTCGGGTCAGGCGTAT‑CCGCCGCGTCAGATATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa < 2:819241/141‑1 (MQ=11) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 2:711067/98‑1 (MQ=255) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg > 1:711067/1‑98 (MQ=255) taacctcgCGTGAATTGTTAGATAGGATCATGGGTTT‑ACTCCGCACCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTAATTACGCATGTTTTTGTAGGTCGGATaa < 2:116617/136‑1 (MQ=2) tGACTTAGCGTGATTTTGTAGGTCGTTTAAGGTGTTT‑ACGCTGGTTTAGATTGCTGCGCCTTATTCAACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa < 1:841031/143‑1 (MQ=11) gATTTAGCGTCATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg < 1:193074/143‑1 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 2:391785/80‑1 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg > 1:391785/1‑80 (MQ=255) ttttGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc > 2:519401/1‑78 (MQ=255) ttttGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAGTGCGTTTATGCCg > 1:163463/1‑143 (MQ=255) ttttGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg > 1:704222/1‑143 (MQ=39) ttGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTCTTTCGCGTGACTTCGTATGTCGGACAAGGCGTCTATGCCGCa > 2:63714/1‑143 (MQ=14) ggTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGa > 1:27378/1‑143 (MQ=25) gTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc < 1:606940/65‑1 (MQ=255) gTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc > 2:606940/1‑65 (MQ=255) tCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAcc > 1:517807/1‑142 (MQ=39) tAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc > 1:568723/1‑143 (MQ=39) aaGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACg > 1:877872/1‑143 (MQ=39) ttt‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 1:192186/56‑1 (MQ=255) ttt‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 2:192186/1‑56 (MQ=255) aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAGTAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCg > 2:235109/1‑143 (MQ=255) ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa > 2:369863/1‑47 (MQ=40) aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATCTTGTAGGTCGGCTAAGGCGCTTATGCCACATCCGACATCAACGCCTGATGCGACGGTTa > 2:410140/1‑143 (MQ=11) tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCACCGCCTGCTGCCACGCtgac > 1:222884/1‑140 (MQ=255) tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGATAATGCCGCATCAGACATCGACGCCTGATGCGACGcgtgg > 1:169845/1‑139 (MQ=11) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt > 1:546230/1‑143 (MQ=18) | TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCT > NC_000913/4295918‑4296198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |