| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,470,958 | G→T | 11.2% | intergenic (‑45/+28) | ridA ← / ← pyrI | enamine/imine deaminase, redox regulated chaperone/aspartate carbamoyltransferase, PyrI subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,470,958 | 0 | G | T | 11.2% | 120.2 / 9.7 | 54 | intergenic (‑45/+28) | ridA/pyrI | enamine/imine deaminase, redox regulated chaperone/aspartate carbamoyltransferase, PyrI subunit |
| Reads supporting (aligned to +/- strand): ref base G (20/28); new base T (2/4); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01 | |||||||||||
ATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGG > NC_000913/4470816‑4471100 | aTCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATCATAgg > 2:677661/1‑143 (MQ=255) tCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCAGTAAAAAATATGGCACATTATAggg > 1:613620/1‑143 (MQ=255) gcccGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGc < 2:137594/141‑1 (MQ=255) aCCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATGTCTCCTTTATTACAGCGGTAAGAAGTCTCGCTCATTAGAGGGAGcg > 2:344195/1‑142 (MQ=255) cGGAGGTGATGGTCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCTGTCGCGATAGTTTTGCTAATGATTTCTCCTTTATTACACCGGTAAAAACTCTGGCTCATTATACGTAGGCAg > 2:477178/1‑143 (MQ=255) ggAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGa < 1:401960/143‑1 (MQ=255) gAGGTTATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGAc < 1:227206/143‑1 (MQ=255) tgatgaTCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtttt < 1:621660/143‑1 (MQ=255) atgatCATATTGCCCAGTGCTACGCCCTGTACGTAAGGACCGAGAGTTGGCGTGGCATTTTCCGTCGGTATAGGTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGTTCCCTATAATGAGCCAGACttttta < 2:559495/143‑3 (MQ=255) aggctCATATTGCCCGGATCACCGCCTTGTACGTAAGGCCCGCTAGCTGCGGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATGTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACttttta < 2:370539/139‑3 (MQ=25) gatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTCTAGGGAGCCAGACTTttatta > 2:427560/1‑143 (MQ=255) gatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttatta < 1:701521/143‑1 (MQ=255) gtatTGCCCACGTCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGCGCATTTTCCGTCGCGATAGTTTCGCTCATGATTTCTCCTTTATTACCGCGGTAATTAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCaa < 2:202085/142‑1 (MQ=255) tCAGCGCCCTGTACGTAGGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAACGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCcattaatg < 2:880088/143‑2 (MQ=255) cAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaatta > 1:129597/1‑142 (MQ=255) atcGCCCTGTACGTTAGGTCCGTTAGCTGCCGGTGCATTTTCCGGCGTGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTgg < 2:16260/141‑1 (MQ=255) aaCGCCCTGTGCGTAATGACCGTTAGCTGCCGGTGCTTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACTGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTgg < 2:235816/143‑1 (MQ=255) cGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGcc < 2:7999/143‑1 (MQ=255) cccTGTACGTAAGGACCGATAGCCGCCGGTCCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAg < 2:46714/143‑1 (MQ=255) ccTGTACGTCAGGACCAATAGCTCCCCGTGCCCTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCTTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGc < 1:902137/143‑1 (MQ=255) tACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCGTTATAGGGAGCCAGACCTTTATTACTAAACGGAATCAACTGGCCAGaacca > 2:679305/1‑143 (MQ=255) gTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACAt > 2:308595/1‑143 (MQ=255) gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATgg < 1:317433/143‑1 (MQ=255) gagtgCTGTCGGCGCAGTTTCCGGCGCGATCGTGTTGCTGTTGGGTGCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaa < 1:298945/139‑1 (MQ=255) gCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActc > 1:748121/1‑143 (MQ=255) cgCCGGTGCATTTGCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtt < 1:786983/142‑1 (MQ=255) cATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCGAGCACCACATTATGGGAAAAATCTTTTTccaag > 1:722148/1‑139 (MQ=255) ttttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACATTTATCACCAACCGCAATTACTTGGCCAGCACCACATGATGGGAACACTATTATACACAgca > 2:225025/1‑141 (MQ=255) gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGCGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCAGTTTCACAGTATTTACa > 2:20429/1‑143 (MQ=255) tCGCGATAGGTTTGCTCATGATTTGTCCTTTATTACGAAGCTGAAAAGTCTGGCTCATTATAGGGAGCCGGACGCTTAAGACCAACCGCAATCAATTTGCCACCACCACATGATGGGAAAACTCTTTTTCACAGTATTGGCAt > 2:239494/1‑143 (MQ=255) gcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGTCTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAttt < 1:308595/143‑1 (MQ=255) gTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:849950/143‑1 (MQ=255) ttttGCTCATGATTTCTCCTTTATTACCGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGcgcg < 1:859295/143‑1 (MQ=255) tttGCTCATGATTTCTCCTTTATTACAGCGTTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTCCCCACTATAATTAGTTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa < 2:871844/143‑1 (MQ=255) ttGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCATTTTCACAGTATTTGCATTTGAGCGCCat > 2:639470/1‑143 (MQ=255) cttGATTTCGCCTTCCTTACAGCGTTAGAAAGTCTGGCTCATGATAGGGAGCCTCACTTTCATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt < 2:280997/141‑1 (MQ=255) tttCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg > 2:578142/1‑143 (MQ=255) gccgtttttaCAGCGGGAAAAGGCCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt < 1:499254/136‑1 (MQ=255) gccTTTATTACAGCGGTAAAAAGGCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAATCTCGTGTTCTTAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt < 1:179021/142‑1 (MQ=255) ccTTTATTACATCTGTAAAAAGTCTGCTGCATTCTAGGGAGCAAGGCTTTAGCCTCCATGCGGAATTCGTTCGCAAGCACCACATTATGGGAATACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt < 2:918897/143‑1 (MQ=255) tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc < 2:209768/143‑1 (MQ=255) tattaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGTATTTGCGCGCGATAGCCTTGGCGCGGTTTCgg > 1:585057/1‑142 (MQ=255) aGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAACACTCTTTTTCCCAGTATTTGCATTTGAGCGCGCTCTCCTTGGCGCCTTTTCGCACGGCa > 2:96522/1‑143 (MQ=255) aaaaaGTCTGGATCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:221997/143‑1 (MQ=255) aGTCTGGCTCATTATAGGGAGCCAGACTTTTTTTATCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATg > 2:334758/1‑143 (MQ=255) gTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCGTTGGCGCGTCTTCGCACGTCAACACTGGATGa > 1:802069/1‑143 (MQ=255) gTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGCTTTCGCACGGCAAAGCTGGATGa > 1:942628/1‑143 (MQ=255) cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCCAAGCTGGCTGaaa > 2:485391/1‑143 (MQ=255) tGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTTTTTTTCACAGTATTTGCGTTTGAGCGCGCTATCATTGGCGCGGTTTCGCACGGCAAAGCTGGGTGAAAc > 1:362515/1‑143 (MQ=255) tGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaaa > 1:708011/1‑131 (MQ=255) tGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaaa < 2:708011/131‑1 (MQ=255) ggCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc < 1:862894/143‑1 (MQ=255) gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCg < 1:907164/143‑1 (MQ=255) tCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATAAAACCGGt > 2:911572/1‑143 (MQ=255) cATTATAGGGAGCCAGACTGTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCGTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt < 1:6363/143‑1 (MQ=255) aTTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc < 1:400153/143‑1 (MQ=255) gggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGCGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg < 1:751789/143‑1 (MQ=255) ggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg > 1:759046/1‑143 (MQ=255) | ATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGG > NC_000913/4470816‑4471100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |