| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,472,437 | T→C | 8.1% | N33D (AAT→GAT) | pyrL ← | pyrBI operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,472,437 | 0 | T | C | 8.1% | 114.3 / 2.3 | 50 | N33D (AAT→GAT) | pyrL | pyrBI operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (25/20); new base C (2/2); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01 | |||||||||||
TTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGC > NC_000913/4472296‑4472579 | ttGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTg > 2:766389/1‑143 (MQ=255)ttGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTg > 2:118848/1‑143 (MQ=255) tttCAGTTTCGCCGCTGTCGCCAGCCCCAGATTCAGGTCATCGCGACCAAAGTCGTTTATGGAAATGATATGTTTCAGATATAGCGGATGAGCCATCTTTTATCTCCTGACGCCTTGGCAAGAAAAAGCCCCTCGCTTGAggg > 2:902282/1‑143 (MQ=255) ttCAGTTTCGCCGCTGTCGCCAGCACTAGATTAAGGTTATAGCGACTCAGGTCGTTTATGGACATGATGTGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCATGACGCCTGGGCAAAAAACAGCCCCTCGATCTAgggg > 2:286551/1‑143 (MQ=255) ttCAGTTTCGCCGCTGTCGCCAGCACCAGCTTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGAGTAGCCATCTTTTATCTCCTGACGCCTGGGCACAAAAACGCCCCGCGATTGAgggg > 1:283483/1‑143 (MQ=255) ttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAgggg < 1:61117/143‑1 (MQ=255) tCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGACATCTTTTATATCCTGACGACTGGGCAAAAAAAAGCCCCTGGATTGCGGGGCTGGGaa > 2:938418/1‑143 (MQ=255) tCGCCACTGTCGCCAGCACCAGATTGATGTCATCGCGACTAAGGTCGTTTATGGAAAGGATATGTTTCTGATATCGCGGATTAGCCATCTTTTATCTCCGGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttttt < 2:871250/143‑6 (MQ=255) ccgcTGTCGCCAGCACCAGATCACGCTCATCGCGCCTCAGGTCGTTTCTCGCAACGCTCTGTTTCTGCTACCGCGGACTAGCCATCCTTTACTCCCTGCCGCCTGGGCACAGAAGAGGCCCACTCTTGACGGCCTggatatgc > 2:147317/1‑137 (MQ=255) tGTCCCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATTTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGa < 1:40927/143‑1 (MQ=255) ggTCGCGAGCAGCAGGCTAGGGTCATCGCGACTAAGGTTGGTTGTGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGa < 2:57612/142‑1 (MQ=255) cAGCACCAGATTAAGGGCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACg < 1:203099/143‑1 (MQ=255) cACCAGTTTCAGGCCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGga < 1:835440/143‑1 (MQ=255) aCCAGATTAAGGTCATCGCGACTAAGGCCGTTTATGGAAATGATATGCGTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaa < 1:516291/143‑1 (MQ=255) cAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAGCGGGTGCTCAACGCgaaga > 2:767452/1‑143 (MQ=255) aTCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGc < 2:393374/143‑1 (MQ=255) aTCGCGACGAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGc < 2:48683/143‑1 (MQ=255) tCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGAGTGCTATAAGGTTTGGAAAACGGCAGGcc > 1:725728/1‑143 (MQ=255) gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCACAAAAAGGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttttt > 1:793648/1‑143 (MQ=255) gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGCAGAAACACGGCAGGCCAGCGTCttttt > 1:701047/1‑143 (MQ=255) tGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAcgc < 1:369853/143‑1 (MQ=255) ggAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCAAGCATCTTTTTTCAGAcgcg > 2:700429/1‑143 (MQ=255) aaaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCCGt > 2:820129/1‑143 (MQ=255) tGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGCATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAg > 2:418475/1‑143 (MQ=255) ttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATg < 1:766389/143‑1 (MQ=255) ttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATg < 1:118848/143‑1 (MQ=255) tCTGATATAGCGGACTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt < 1:244757/143‑1 (MQ=255) ccgATATGGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCGGGGAATGGGGGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc < 1:341231/141‑1 (MQ=255) atgtaGCAGATTAGCCCTCTTCCGTGTCTTGACCCGGGGGCAAAAAAAAGCCCCTCGATGGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa < 2:862768/140‑1 (MQ=255) aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca < 1:358036/143‑1 (MQ=255) gCCACCTTTCAACTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt < 1:333558/143‑1 (MQ=255) ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGCGGGGCCGGGGATGGGTGATCAACGGGAAGAAAAACGGCAGGCCCGCGTCTTTGTGCAGACGCGGTAAGACAAAATGTCAGACGCCCTGAACCATa > 2:897465/1‑143 (MQ=255) cATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAGGACAAAATGTCGGACACACTGAAccctcc > 1:170553/1‑139 (MQ=255) aTCTTTTATCTTCTGACGTTTTGGTATAAAAAAGCCCCTCGTTTGCGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACa < 1:79645/143‑1 (MQ=255) aTCTTTTATCTCCTGACGCCTGGGAAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAACAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAAGGTCGAACACAGTGAACAATACa > 1:596758/1‑143 (MQ=255) aTCTTTTATATCCTGACGCATGGGCAAAAAAAAGCCCCTCGATTGAGGTGCTATGCAGGGGCTAACAGCGGGCAGAAAAACGGCAGGGCAGCGTGTTTTCTTAGACGAGGTAAGACTAAATGTCGAACACACTGAACCATACa > 2:221928/1‑143 (MQ=255) ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGCACACAGTGAACCGTACAtcc > 1:140111/1‑143 (MQ=255) aTCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTccc < 1:791581/143‑1 (MQ=255) tcCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGCTCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACGCTGAACCATACACCCTCCCGGc > 2:556203/1‑143 (MQ=255) gCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCAGGCAAATTgc > 2:586216/1‑142 (MQ=255) gCCTGGGC‑AAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTACCGGCAAATTTTc > 2:735325/1‑143 (MQ=255) cTGGGCAAAAAAAAGCCCCTCGATTGGGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTcc < 1:829966/143‑1 (MQ=255) gCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAACTTGTCCGGCa < 1:604193/143‑1 (MQ=255) gCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCCTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATCGTCCGGCa < 1:257152/143‑1 (MQ=255) aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCGAATTGTCCGGCAtt > 1:555282/1‑143 (MQ=255) aaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGGCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATta > 1:611557/1‑143 (MQ=255) agaaGCCGCGCGAGTGAGGGGCTGTGAATGGGGGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATAc < 2:610614/141‑1 (MQ=255) ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGCTCAACGCGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCACATTGTCCGGCATTATACt > 2:287094/3‑143 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACGTCCTCCCGGCAAATTGTCCGGCATGATACTCATCGTc > 2:785753/1‑143 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCgtcgtc > 1:739873/1‑143 (MQ=255) tGAGGGGCTGGGAGTGGGTGACCAACGGTAAGAAACACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAACTTGTCCGGCATTATACTCATCGTCAGAAgc < 2:796591/143‑1 (MQ=255) | TTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGC > NC_000913/4472296‑4472579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |