| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,196,268 | Δ2 bp | intergenic (‑169/+63) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,268 | 0 | C | . | 94.9% | 235.4 / 6.0 | 60 | intergenic (‑169/+64) | thiC/rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base . (36/20); total (37/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,196,269 | 0 | C | . | 96.5% | 234.9 / 3.0 | 60 | intergenic (‑170/+63) | thiC/rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (36/20); minor base T (1/1); total (37/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGC > NC_000913/4196139‑4196403 || acgGGTGAAGCAGGAGACGGTCGCCGCAGGGGCGGTGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATGAGCCTGATCAGGTTCCGCGGATCCCGAATAAACTGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGc < 2:340520/143‑1 (MQ=255) ggATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCCGGCTCCGCGGACCCCGCTTACCCGGTCTCTGCCAG‑TCAGGGAC‑ATGACAAGAAACTAGccc > 1:241233/1‑143 (MQ=255) aggAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCg < 1:302936/141‑1 (MQ=255) aGCAAGAGACGATCGCCGCAGGGGCGATGCATTAAATGTCGGATGCGAGTTCCCTTCGCAGGTATTAGCATGATCAGGGACCGCGGAGGCCGAAAAAACGTACTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCaaa > 2:394837/1‑143 (MQ=255) gagaCGATCGCTGCAGGGGCGATGCGATGGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTTCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATgggg < 2:131424/143‑1 (MQ=255) agaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGc < 2:30787/143‑1 (MQ=255) gATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCGTTAGGGCGCGGTACCCTGCTAAGGCGCCGCTGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTg < 2:494325/143‑1 (MQ=255) gATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTg > 2:1047137/1‑143 (MQ=255) aTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCACATGGGGCATTGa > 2:816596/1‑143 (MQ=255) cAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTCCGCATGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTaaa < 1:756950/143‑1 (MQ=255) gggCGATGCGATAGCAGATTAGCTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTa < 2:467171/143‑1 (MQ=255) cgatgagatAGCAGATTAACGATTGTTCCCTTCGCAGGTATTATCCTGATCAGTTTCCGCGGATCCCGAATAACCGGTCTCAGCCAGTTAAGGCATT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTAcgc < 2:425695/143‑1 (MQ=255) gatgcgatAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAACTAGACCCGCAAGTGGGGCATGGAATGTAAATTCcgcg > 2:123509/1‑143 (MQ=255) tgcgatAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGtt < 2:1020994/143‑1 (MQ=255) aGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAgc > 2:420084/1‑143 (MQ=255) ctctTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCa > 1:664757/1‑143 (MQ=255) tctTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGAGGCATTGAATGTAAAGTACGCGTTAACAGCGCAAAACTCaa > 2:835520/1‑143 (MQ=255) ttGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCCAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGc > 2:710556/1‑143 (MQ=255) ttCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAgg > 2:715709/1‑143 (MQ=255) cccTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGAGCGGCAAATGGGGCATGGAATGTAAATTACGCGTTAGCAGCGCAGAACACAAGCAGGAt > 1:933968/1‑143 (MQ=255) gCAGGTATTAGCCTGATCAGGTTAAGCGGATCCCGAATAAACGGTCTCAGCAAGTTAAGGCACT‑‑GACAAGAAAGTAGCGCGGCAAATGGGAAGTAGAATGTAAATTACGCGTAAACAGCGCAGAACTCAAGCAGAATGGTTGa > 2:1072423/1‑143 (MQ=255) aGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATCGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGAcg > 2:659317/1‑143 (MQ=255) gTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGCcgcg > 1:393242/1‑143 (MQ=255) gatgaGCCTGATCAGGTGCCGCGGATCCCGAATAAACGGGCTCAGCCAGTTAAGGAA‑‑CAGTTAAGAACTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcgg < 2:668876/139‑1 (MQ=255) aTTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcggg > 1:833094/1‑143 (MQ=255) gCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCTCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc < 1:102078/143‑1 (MQ=255) gCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc > 2:514009/1‑143 (MQ=255) gCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc < 1:1003875/143‑1 (MQ=255) ccTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCAGTGAATGTAAAGTACGCGCTAACAGCGCAGAACGCAAGCAGGATGGTTGACgagggcgggg > 1:332454/1‑141 (MQ=255) cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACCAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGCTGCTTGACGCGGGCGGCGt > 1:453463/1‑143 (MQ=255) cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGt > 1:577469/1‑143 (MQ=255) tCAGGGTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTCGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 2:549963/143‑1 (MQ=255) cAGGTTCCGCGGATCCGGAGTAGGCGGTCTCGGCGGGTTAAGG‑‑GTCTGGCGGGCGGTTAGGCCCGCGGTTGGGGCATTGAATGTAAATTACGCGTTGGCGGCGCCGCTCTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGc < 2:21353/143‑1 (MQ=255) ggTTCCGCGGTTGCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAc < 2:576180/143‑1 (MQ=255) gTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGGATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAcc > 1:141917/1‑143 (MQ=255) tCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACTAGAAATTAGCCCCGCAACTGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcag > 2:287432/1‑143 (MQ=255) cgcgGATCCCGAATAAACGGTCTGAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca < 1:394325/143‑1 (MQ=255) cgcgGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca > 1:502592/1‑143 (MQ=255) aTCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATc < 1:622784/143‑1 (MQ=255) ccGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAGCTCAAGCAGGATGTTTGACGCGCGCGGCGTCACGCACCAGCAGCATCGGc > 2:105646/1‑143 (MQ=255) cGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACCAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGAGCAGCGTCAAGCACCAGCAGAATCAGCt > 2:412761/1‑143 (MQ=255) cGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCt > 1:1063152/1‑143 (MQ=255) cGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCt > 2:79645/1‑143 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAAGTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCtt < 1:394837/143‑1 (MQ=255) gAATAAACGGTCTCAGCCAGATAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGAATTGAATGTAAATTACGCGTTAACAGCGCAGCGCTCAAGCAGCATGTTTGACGCGGGCGGCGTAAAGCACCAGCAGAATCAGCta > 1:535347/1‑142 (MQ=255) aaCGGTCTCAGCCAGTTAAGGCACT‑‑GACGAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACACCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCCCCTGCAGAATCAGcgcacctt > 2:475449/1‑136 (MQ=255) aCGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGTGCATTGAATGTAAATTACGGGATAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTc > 1:911166/1‑143 (MQ=255) aCGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTc > 1:865654/1‑143 (MQ=255) aCGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTc > 1:176174/1‑143 (MQ=255) cGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTcc < 2:815613/143‑1 (MQ=255) ctcAGCCAGTTAAGGC‑C‑CGGACAAGAGAGTTGCCCGGCAAGTGGGGCATTGAATGTAAATTGCGCGTTGACAGCGGGGAACTCAAGCGGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGca < 2:186845/143‑1 (MQ=255) cAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCaa < 2:958404/83‑1 (MQ=255) cAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCaa > 1:958404/1‑83 (MQ=255) aGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcacca > 1:825426/1‑141 (MQ=255) aGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACaa > 1:404433/1‑143 (MQ=255) aGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACaa > 2:810993/1‑143 (MQ=255) aGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCACCGCAAATGGGGCATTGCATGTAAATTACGCGATAACAGCGCAGAACTCAAGCACGATGTTTGACGCGGGCCGCGGACAGCCCCCACAGAACCACCGTAACTTCCAGCACaa > 2:214500/1‑143 (MQ=255) gTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgc > 1:591445/1‑143 (MQ=255) gTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgc < 1:593315/143‑1 (MQ=255) tAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcgc < 2:833094/143‑1 (MQ=255) || ACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGC > NC_000913/4196139‑4196403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |