| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 96.5% | 149.0 / NA | 60 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (28/28); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGA > NC_000913/125246‑125523 | gagaTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCa > 1:346291/1‑143 (MQ=255) agaTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAg < 1:447992/143‑1 (MQ=255) tGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCCGGAACATGCTGCACCCGCTGGAAACACCGCGCGCTCCGCATATCGCTAAAGcgatg > 2:944364/1‑143 (MQ=255) gCGAACGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATGGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGaa < 1:977263/143‑1 (MQ=255) cGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCACGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGCGGATGAac > 1:617561/1‑143 (MQ=255) gaaGAGTACGGCGTAGGTGCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacga < 2:752433/142‑1 (MQ=255) aaaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGGAACTCCGCGCGTTCCGTATAGCGCTTAGGTGAGGAacga > 1:623256/1‑143 (MQ=255) aaaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacga > 1:642059/1‑143 (MQ=255) cGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCgg < 1:244661/143‑1 (MQ=255) ggCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGAGGc > 1:1045594/1‑137 (MQ=255) ggCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGc < 2:1045594/137‑1 (MQ=255) ggTTCGGACGTGTATAGCGTGACCGCCTTCACCGAGCTGGCGCGTGATGGTCAGGAGTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGc < 2:947088/143‑1 (MQ=255) ttCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCACATAGGTGATGAACGACGCTCCGGCAGTGCCAt > 2:460803/1‑143 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGAACGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGCACAACGCCCAGGCAGCGGCATCCAcc > 2:426392/1‑143 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGa > 1:136635/1‑143 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGa > 2:630029/1‑143 (MQ=255) cGTGACCTCCTTCACCGGGCTGGCGCGTGATGGTCAGGGTGGCGCACGCTGGAGCCAGCGGCACCCGCCGGGAACTCAGCGCCGTCCGGATAGCGGTGAGGAGAGGAACGACGCGCCGGCAGTGGCAGCTACCGACtgtcgga > 2:1048269/1‑137 (MQ=255) cGTGACCTCCTTCACCGAGCTGGCGCGTGCTGGTCAGGATTGTGAACGCTGGAACATGCTGCACGCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACAACGCTCCGGCAGTCGGATCTACCGACtatcaga > 2:1009467/1‑139 (MQ=255) tggccgcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGaaa < 2:622942/137‑1 (MQ=255) tGACCTCCTTCGCCGGGCTGGCGCGTGATGGTCAGGATTGTGCACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGaaa < 2:1088912/143‑1 (MQ=255) gACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAAc > 1:330327/1‑143 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGtt > 1:793775/1‑143 (MQ=255) cctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACAGACTATATGAAACTGTTc > 1:285470/1‑143 (MQ=255) tCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCt < 1:87463/143‑1 (MQ=255) aCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACCCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCACCGGCAGTGGCACCTACCGACTATATGAAACTGTTCGCTgg > 2:973877/1‑142 (MQ=255) ccGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAg < 1:322854/143‑1 (MQ=255) cGAGCTGGCGTGTTGTGGTCAGGATTGCGAAGTCTGTAACATGCTGGTCCTGGTGGAAGCTCCGCGCGTTCCGTGTATCGCTTAGGTGAAGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGc < 2:576257/143‑1 (MQ=255) aGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTCCCGACTATATGAAACGGTTCGCTGGGCAg > 2:750152/1‑143 (MQ=255) tgccgcgTGATGCTCAGGATTGTGAACGCGTGAACATGCCGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATCAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTc < 1:473628/140‑1 (MQ=255) ggCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTcc < 1:957661/143‑1 (MQ=255) ggCGCATGAGGGTCAGGATGGCGCACGCGGGAACAGGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTcc < 1:134190/143‑1 (MQ=255) gcgcGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTTCGCGCGTTCCGTATATCGCTTAGGTGATGAAAGACGCTCCGGCAGTGGCATCTACCGACTACATGAAACTGATCGCTGCGCAGGTcca > 2:571160/1‑142 (MQ=255) aTGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTAc > 1:1029684/1‑143 (MQ=255) ggTCAGGATTGTGGACGCTGGAGCATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGt < 1:369012/143‑1 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCCGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTa > 1:650284/1‑143 (MQ=255) tCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTag > 2:1053090/1‑142 (MQ=255) tCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAc > 1:1012486/1‑143 (MQ=255) aGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCg < 1:368766/143‑1 (MQ=255) aGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATAACGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACGGACTATATGAAACTGTTCGCTGAGAAGGTACGTACTTACGTAGCg > 1:367441/1‑143 (MQ=255) tgctgAACGCTGGAACATGCTGCCCCCGCGGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTg < 2:1006928/140‑1 (MQ=255) gCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAc > 1:344686/1‑143 (MQ=255) cTGGAACAGGGTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc < 1:527988/143‑1 (MQ=255) tGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTAGATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcg > 2:903136/1‑143 (MQ=255) gAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgcg > 2:887083/1‑143 (MQ=255) caggctgcACCCGCTGGAAACGACGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTAc < 1:1038713/140‑1 (MQ=255) gcagcACCCGCTAGAAACCCGGCTCGGTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg < 1:750152/140‑1 (MQ=255) ctgcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTggg > 1:84142/1‑143 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa < 2:793775/143‑1 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGACGGACGACGCTCCGGCAGTGGCATCTACCGCCTATATGACACCGATCGCTGAGCAGGCCCGTACTTACGTACCGGCTGACGACCACCCACTACTggggcctgt > 2:486980/1‑137 (MQ=255) tGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGc > 1:24098/1‑143 (MQ=255) tGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGc > 1:570495/1‑143 (MQ=255) gAAACTCCGCGCGTTCCGTATATCGCGTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCtt < 1:39427/143‑1 (MQ=255) aaCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGCACCGGGTACCGGTCGCTTCg > 2:248973/1‑143 (MQ=255) ccGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCg < 1:335160/143‑1 (MQ=255) cgTTCCTTATATCGCTTAGGTGATGAGCGACGCTTCGGGAGGCGCACCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCg < 2:1081816/143‑1 (MQ=255) gTTCCTTATATCGCTTAGCTGCTGCACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGTTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGTGTACTGATGGCTTCGGTCGTTCCGa < 1:474586/143‑1 (MQ=255) ttCCGTATACCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGAc < 1:47882/143‑1 (MQ=255) atatCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 2:570495/143‑1 (MQ=255) atatCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 2:344686/143‑1 (MQ=255) atCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTGCTGATGGCTTCGGTCGTTCCGACAGCCGTga > 1:932564/1‑143 (MQ=255) | GAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGA > NC_000913/125246‑125523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |