Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,969	C→G	V55L (GTT→CTT)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,969	0	C	G	100.0%	161.9 / NA	54	V55L (GTT→CTT)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (33/21);  total (33/21)

GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661829‑2662107                                                                                                                                             |                                                                                                                                           gccgccCTTGCTGTCTCCTTTACCCTGACAACGGGTGCCATCGACAGATTCGTCAACGGCGCTAATTTCTTCGCCCACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGAATGATCCACGTACGATGGAAAGCa                                                                                                                                          <  2:451228/143‑1 (MQ=255)  ccgccCTGGTAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAg                                                                                                                                         <  1:934527/143‑1 (MQ=255)     cccTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCGACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACAACCGCCTGGTGCCCGTACGCTGGCAAGCAGAcc                                                                                                                                      >  2:359573/1‑143 (MQ=255)       cTGGCTGCCGCCTTTCCCCTGCCAACGGTTGGCTTCTACAGATTCGTCAACGGCGCTAGTTACTTCGCCTACGGCGATGCTGCTGGCATCGTTGCCTAACAGATACCCACCGCCTGGTCCACGTACGCTGGAAAGCAGAccct                                                                                                                                    <  2:325714/143‑3 (MQ=255)        tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtt                                                                                                                                   <  1:936854/143‑1 (MQ=255)         ggCAGCCGCCTTTGCCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAttt                                                                                                                                  <  2:107900/143‑1 (MQ=255)             gccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCCCCGCCTGGTCCTCGTACGCTGGAAAGCAGACCATCTTTaa                                                                                                                              >  2:962182/1‑142 (MQ=255)                  cTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGa                                                                                                                         >  1:905718/1‑143 (MQ=255)                      aCCCCGACAACGCGTCGCATCCACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACggg                                                                                                                     <  1:579739/143‑1 (MQ=255)                        ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGCACGCTGGAAAGCAGCCCATTTTTACCCAGACgggga                                                                                                                   >  2:763816/1‑141 (MQ=255)                          tggcAAGGGGTGGCATGTACGGATGCGTCAACGGCGCTAATTACGTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaaa                                                                                                                 <  2:214130/140‑1 (MQ=255)                                 gggTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgtt                                                                                                            >  2:418087/1‑141 (MQ=255)                                 gggTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgtt                                                                                                            <  1:418087/141‑1 (MQ=255)                                     ggCATCTTCAGACTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTTACATATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCa                                                                                                      <  1:938894/143‑1 (MQ=255)                                       cATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACCGTTGTTCCAGa                                                                                                    >  1:330877/1‑143 (MQ=255)                                       cATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa                                                                                                    >  1:392163/1‑143 (MQ=255)                                        aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCCTTGCCTAACAGATAACCACCGCCTGGTCCACGAACGCTGGAAAGCAGACCATCTTTACGCAGACGGGAAAACAGATGTTCCAGac                                                                                                   >  2:608645/1‑142 (MQ=255)                                           tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAg                                                                                                >  2:695333/1‑143 (MQ=255)                                            aCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGa                                                                                               >  1:762763/1‑143 (MQ=255)                                             cAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTCCGCAGCCGGGAAAACAGTTGTTCCAGATCAGaa                                                                                              >  2:267003/1‑143 (MQ=255)                                                     tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGACAGCAGACCATTCTTACGCCGACCGGAAAACAGCTGTTCCGGATCAGCGAGGGAAAt                                                                                      >  2:574080/1‑143 (MQ=255)                                                     tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                      >  1:121393/1‑143 (MQ=255)                                                     tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                      >  1:238815/1‑143 (MQ=255)                                                          ggCGCTACGTACGTCGCCAACGGCGATGCGGGTGGCAGCGTTGCCTAACAGATAACCACCGCCTGGTCAACGTAAGCTGGAAAGCAGAGCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAGAAGAAAGGGAAATTGCCt                                                                                 <  2:477795/143‑1 (MQ=255)                                                                 aTTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTc                                                                          >  1:665526/1‑143 (MQ=255)                                                                  ttACTTCGGCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                                                                         <  2:89294/143‑1 (MQ=255)                                                                      ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGCAATTCCCTGACGTTCGGaaa                                                                     >  1:457353/1‑143 (MQ=255)                                                                      ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                     >  1:204527/1‑143 (MQ=255)                                                                         gCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCAGCGGCTGGTCCACGTCCGCAGGCAAGCAGACCGTTTTTACGCAGTCGGGAAAGCAGTTGTTTCAGATAAGAAACGGGAAGTGCAGGACGTGCGGacctag                                                                  >  2:854058/1‑138 (MQ=255)                                                                           catcGGCGATGCGGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCTTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                                <  2:508109/140‑1 (MQ=255)                                                                           cagcGGTGACGCTGCTTGCATCTTTGCCTAACAGATTACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                                <  2:319834/140‑1 (MQ=255)                                                                           cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAAGATCa                                                                >  2:873467/1‑143 (MQ=255)                                                                                            gCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGcc                                               <  1:953449/143‑1 (MQ=255)                                                                                               tCTTTGCCTAACAGAGAACCACCGCCGGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGc                                            <  2:999352/143‑1 (MQ=255)                                                                                                 tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGCAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTCCGGAAATATCAGCCCACGGTAGCGGGCCCGCtt                                          >  2:122398/1‑143 (MQ=255)                                                                                                  ttGCATAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTGACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTc                                         <  2:891815/143‑1 (MQ=255)                                                                                                   cgCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGCAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                        <  1:671075/142‑1 (MQ=255)                                                                                                        aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACTGGAAAGCGGCTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCACACGGGACCGCGCCCGCTTCaggggt                                   >  2:673619/1‑139 (MQ=255)                                                                                                         aCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCagagcag                                  >  2:51756/1‑140 (MQ=255)                                                                                                           aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg                                <  2:204527/143‑1 (MQ=255)                                                                                                             aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                               <  1:1003544/142‑1 (MQ=255)                                                                                                             aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                               >  2:1003544/1‑142 (MQ=255)                                                                                                             aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGATGAgcg                              >  2:141846/1‑143 (MQ=255)                                                                                                                  caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAGAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                         >  1:351290/1‑143 (MQ=255)                                                                                                                  caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                         >  1:313094/1‑143 (MQ=255)                                                                                                                  caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGGACCGGGCCCGCTTCCGAGACGAGCGCAACg                         >  2:891511/1‑143 (MQ=255)                                                                                                                           gTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGCTGAGCGCAACGTCAAGCAtt                >  2:854907/1‑143 (MQ=255)                                                                                                                             ccACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATAACAGCCAACGGTACCGGGCGCGCTTCAGGGTTGAGCGGAACGTCAAGCATTgg              >  1:62028/1‑142 (MQ=255)                                                                                                                               aCGTACGCTGGAAAGCAGACCATTTCTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCgg            >  2:816591/1‑143 (MQ=255)                                                                                                                                     gCTGGAAAGCAGCCCATTTTTCCGCAGACGGCACAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCTGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg      <  1:86033/143‑1 (MQ=255)                                                                                                                                     gCTGGAAAGCAGACCATTTTTACCCAGCCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCCACGTCAAGCATTGCGGTCACgg      <  1:242521/143‑1 (MQ=255)                                                                                                                                       tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTGAAGCATTGCGGTCACGGCa    >  2:406663/1‑143 (MQ=255)                                                                                                                                         gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCCTTGCGGGCACGGCAtc  >  1:936278/1‑142 (MQ=255)                                                                                                                                         gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa  >  2:802430/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                           GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661829‑2662107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑