Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	93.3%	119.7 / NA	45	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/23);  minor base G (0/3);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGA  >  NC_000913/696332‑696609                                                                                                                                           |                                                                                                                                            tCGCATCCGACATTGACGGATAAGGCCGCTCACTGGCGGGTTGGATATTGAATGAACGCAGAAAAGCAAGAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa                                                                                                                                         <  2:489031/143‑1 (MQ=25)   gCATGCAACATTGAAGGATAAGACGTGTCAACATCGCATCCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATc                                                                                                                                       <  1:294057/143‑1 (MQ=255)       ccGACATTGAAGTAGAAGACGTGTCAACATCGCATTCGCCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                   <  1:69564/143‑1 (MQ=255)        cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCCAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                  <  1:395304/143‑1 (MQ=255)                aaGGATAAGACGTGTCAACATCGCATTCGACATAGCCTTTACAAAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGACTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGATGACTTACCg                                                                                                                          >  2:795649/1‑143 (MQ=25)                 aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGc                                                                                                                         >  2:45755/1‑143 (MQ=255)                    aTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCCAGCTTTTTTAATGTCGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTg                                                                                                                      <  1:847889/143‑1 (MQ=255)                     tAAGCCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                     <  1:852713/143‑1 (MQ=255)                     tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                     >  2:808245/1‑143 (MQ=255)                      aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                    <  1:427854/143‑1 (MQ=255)                        gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGa                                                                                                                  >  1:972568/1‑143 (MQ=255)                           gtgtCAACAGCGCATCCGACATGGAATGCAGGGAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAc                                                                                                               <  1:206992/143‑1 (MQ=21)                             gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGACAccg                                                                                                             >  2:208668/1‑142 (MQ=255)                                    gcgcgTTCGAAAGTGGAGGAACGCATAAACGCAAAAAGCTCGCCGAAGGGAGCTTTGTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                      <  2:562258/142‑1 (MQ=18)                                      tcATTCGGTATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                    <  2:592100/142‑1 (MQ=255)                                      gCATTCGACATTGAATGAACGCAGAAAAGCAGAACGCTCGCCGAAGCGAGCTTTTGTAATGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                    <  2:752144/143‑1 (MQ=25)                                       cATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                   <  1:665751/143‑1 (MQ=255)                                         ttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTCGGTGCa                                                                                                 >  1:486939/1‑143 (MQ=255)                                            gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                                                                                              >  1:587170/1‑143 (MQ=255)                                                               aaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                     >  2:716312/1‑101 (MQ=255)                                                               aaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                     <  1:716312/101‑1 (MQ=255)                                                                 aGCAAAAAGCTCGCCGAAGCGGGCTTTTTTAATGTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATa                                                                         <  1:441984/143‑1 (MQ=255)                                                                    aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                      >  1:956318/1‑143 (MQ=255)                                                                    aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGCGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTCGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTCCAAGGTCAGCGGCTTgtcgaaac                                                                      >  2:451117/1‑136 (MQ=21)                                                                     aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGAGTATTGAATAAAtt                                                                     >  1:826659/1‑143 (MQ=255)                                                                          cTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTg                                                                >  1:361957/1‑143 (MQ=255)                                                                             gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCGGGTGCACTTCCATGTGACGCGCCTTGAAGACATCGCCTggcg                                                             >  2:540569/1‑141 (MQ=17)                                                                                  aGCGTGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTCAAACCCGGTGTCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                        <  2:129850/143‑1 (MQ=25)                                                                                      aGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                    >  1:510870/1‑143 (MQ=255)                                                                                       gCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATCGGCTGGGGTACGAGGAtt                                                   >  1:116993/1‑143 (MQ=255)                                                                                         ttttttAATGTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                 <  2:451680/143‑1 (MQ=255)                                                                                                       tGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCTCTTGGCGATCGCTCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                   <  1:870489/143‑1 (MQ=37)                                                                                                        cggctgcGAGGATGCGCACCGAGTAATGCCTCAATTAGAATCCGGTGCATTACCGTTTGGAGGTACCCCAATTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                  <  2:593373/137‑1 (MQ=25)                                                                                                         gggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCgg                                 <  1:478375/143‑1 (MQ=255)                                                                                                          ggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                                <  1:34264/143‑1 (MQ=255)                                                                                                           gTACGGGGGTGCGGACCGCGGAAGGCGGGAATGAGAGTGCGGTGCCTTACCGCTTGGCGATGCCCCAGATGGGTGGACGTACAAGGTAAGCGTCTTGAGGAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaa                               <  2:423187/143‑1 (MQ=21)                                                                                                              ctatgATTCGAATCTCGGAATGCCGGAATTAGTATCCGGTTCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                            <  1:50580/139‑1 (MQ=38)                                                                                                                aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCGGAATCAGa                          >  1:455005/1‑143 (MQ=255)                                                                                                                 ggATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                         <  1:450151/143‑1 (MQ=255)                                                                                                                  gagtCGAACCTCGGAAGGCCGGAATGAGAATCCGGTGCCTTACCGCTGGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                        <  1:282276/140‑1 (MQ=255)                                                                                                                     tcccgccTTGGAATGCCAGAACTAGACACCGGTGCCTTACAGCTTGGCGATACCCCAACTGGGTGCACGCACAAGGTAAGCATCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                     <  2:1027633/138‑1 (MQ=25)                                                                                                                              ggAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCCGCATCCGGTGCCTTAc            >  1:1033304/1‑143 (MQ=255)                                                                                                                                     cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg     >  1:1011622/1‑143 (MQ=255)                                                                                                                                      ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGTATGCCGGAATCAGAATCCGGTGCGTTACAGCTTgac    >  1:267087/1‑141 (MQ=37)                                                                                                                                        aaTGAGAATCCGGTGCCTTACCGGTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGa  <  1:984624/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                            TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGA  >  NC_000913/696332‑696609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑