| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 92.3% | 135.8 / ‑5.5 | 53 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (26/22); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT > NC_000913/759110‑759385 | ccAGGAGACCTTCACCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:1015867/143‑1 (MQ=255)ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:784970/143‑1 (MQ=255) agaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACGCTGGATc > 1:903448/1‑143 (MQ=255) agaCCTTCAACGTCGGTTCAGTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATc < 1:727085/143‑1 (MQ=255) gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATcc < 1:333688/143‑1 (MQ=255) cAACGTCGGTTCATTTGCCAGCGGCGAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAGACGCTGGCTCCGACAGc > 1:1035313/1‑143 (MQ=255) aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATCAGTGCCGAGTATATGGACCTTACCAGCACCGACGAAAACCCCTGGATACGACAACgc > 1:677399/1‑142 (MQ=255) tCGGTTCATGTGCCCGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 1:946019/143‑1 (MQ=255) gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTGCCGAGTATATGCACATTACCAGCGCCGAAGCAAAACGCTGGATCCAACAGCGCACCGAg > 2:647980/1‑143 (MQ=255) ttCATATGCCAGCCGCAGAGAAACCATGAAACTCCGCGACGTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt < 2:521836/143‑1 (MQ=255) cATTTGCCAGCCGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCt < 1:132240/143‑1 (MQ=255) tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTgg > 2:749302/1‑143 (MQ=255) gtacccAGCGGCAAAGACACCATGAACCTCGGCGAGCGGCGGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg < 1:648402/139‑1 (MQ=255) tGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATACAAAAGCGTATCGAGTGTGGTc > 2:208257/1‑143 (MQ=255) ccAGCGGCAAAGAACACATGGAAGTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgc < 2:829944/143‑1 (MQ=255) ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACTCTGGATCCAACAGCGTATCAAGTCTGGTagc > 1:443082/1‑143 (MQ=255) ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 1:612861/143‑1 (MQ=255) gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACt > 1:607325/1‑143 (MQ=255) cgaagaaaccaagaaaccCGGCGAGGTGCTGGAAGCCCGCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACtt < 1:298557/141‑1 (MQ=255) cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGCGTCTGGTCGCGCGACtt > 1:841682/1‑143 (MQ=255) aaaGAAACTATGAAGCTCGGCGGGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt < 2:419606/143‑1 (MQ=255) aaaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTATAGATACATCAGCGTATCGAGTCTGGTCGCGCGACttt > 2:229340/1‑143 (MQ=255) aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAGATGCACATTACCAGCACCGCAGAAAAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTc > 1:996253/1‑143 (MQ=255) aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg < 1:966799/143‑1 (MQ=255) tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCTGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa > 1:116299/1‑143 (MQ=255) gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTGTGGATCCAGCAGCGTATCGAGTCTGGTCGCGCGACTTTCAATCGCGagg > 1:421255/1‑141 (MQ=255) aaaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAaga < 1:889064/143‑1 (MQ=255) actgcgCGAGCCGCTGCAAGCCCTCAACCAAACCTCCTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCACCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAgaga < 2:1017347/138‑1 (MQ=255) aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACACTACCAGCACCGAAGAAAAACTCTGGCTCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAagagc > 2:39311/1‑142 (MQ=255) tctaGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTGCCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa < 1:983009/140‑1 (MQ=255) ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt < 1:1086524/143‑1 (MQ=255) ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAAACTCTGGGTCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGGAACAACGcttctt > 1:899570/1‑143 (MQ=255) tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAACAACGcttcttc > 1:1025364/1‑142 (MQ=255) gAAGCCCTCACGCAAACCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg < 1:1012014/143‑1 (MQ=255) cccTCAAGCACACCGACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 2:1013694/143‑1 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt > 1:107905/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAACAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTGCTTAAGCGAACTg > 1:1075962/1‑143 (MQ=255) cAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAGCAGCGTATCGAGTCTGGTCGCGCGACTGTCAATAGCGAAGAGAAAAAACGCTTATTGAGCGACATGAcaac > 1:482040/1‑140 (MQ=255) cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTg > 2:1116074/1‑143 (MQ=255) cTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAGCGATGGGAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 2:1025364/143‑1 (MQ=255) gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGTAGTGAAAAAACGCTTCTGAAGGGAACTGACCGCCGCTCAAGGt > 1:243169/1‑143 (MQ=255) gTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAc > 1:805231/1‑143 (MQ=255) agcatacGCACATCACACGCACCGAACAAAAACTCTGGATCCAACCGCGTATCTAGTCTGGTCGCGCGACCTTCAATAGCGAAGAGAAAAACCGCCTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc < 1:882472/140‑1 (MQ=255) aTTACCAGCACCGAAGAAAAACTCTAGATCCAACAGCGTATCGAGTCTGGTAGCGCGACTATCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTAAAGGTCTTGAACGTTACCTCGGCGAaaaaacacc > 2:47956/1‑138 (MQ=255) gccAGCACCGAAGAAACGCTCTGGATCCAACAGCGTGTCGAGTCTGGTCTCGCGACTTTCAATAGCGAAGAGAAAAACCGCTTCTTAAGCGAACTGACCGCCGCTCAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTgg < 2:873040/142‑1 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCTAACAGCGTATCGAGGCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGCCAGTTACCTCGGCGCCGAATTCCCTGgcg > 2:802510/1‑143 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGGAGAGACAAAACTCATCTTACAAGGCAGGACCTCCGCGGAAGATCTTGACCGTTACATCGGCGCAAAATTCCCTGgcg > 2:1086077/1‑143 (MQ=255) accACCGAAGAAAAACGCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc < 2:1070519/141‑1 (MQ=255) cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAACACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCGCGGCGCACAACTCCCTGGCGCac > 2:754279/1‑142 (MQ=255) cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgcgc > 1:69822/1‑141 (MQ=255) ccGAGGAAAAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAACCGCTGCTTAAGCGAACTGACCGCCGCTGACGGTCATGAACGCCACCTCGGCGCAAAATTCCCTGGCGCaaaa < 2:677399/143‑1 (MQ=255) cGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAAc > 1:13754/1‑143 (MQ=255) aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGc > 2:387533/1‑143 (MQ=255) aagaaAAACTCCGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGc < 1:647980/143‑1 (MQ=255) gaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt > 1:596367/1‑143 (MQ=255) | CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT > NC_000913/759110‑759385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |