Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,476:1 +C 5.6% coding (56/1215 nt) iscS ← cysteine desulfurase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,4761.C 5.6% 377.5 / 17.1 90coding (56/1215 nt)iscScysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base . (42/43);  new base C (2/3);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

TTTCACGCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCC  >  NC_000913/2661327‑2661623
                                                                                                                                                      |                                                                                                                                                   
tttCACGCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCaa                                                                                                                                                      >  1:1128675/1‑150 (MQ=255)
 ttCACGCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAa‑c                                                                                                                                                    <  1:285546/150‑1 (MQ=255)
  tCACGCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAa‑ca                                                                                                                                                   <  2:1551420/150‑1 (MQ=255)
  tCACGCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAa‑ca                                                                                                                                                   <  2:54787/150‑1 (MQ=255)
      gcgGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CAcgcg                                                                                                                                               >  1:171521/1‑150 (MQ=255)
        ggATCAGCGCCGACCAGATCGTCAATCTGATTACGGGCTATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCggg                                                                                                                                             <  1:1354223/150‑1 (MQ=255)
         gATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGt                                                                                                                                            >  1:195817/1‑150 (MQ=255)
           agattgcCGATCTGGTCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTcc                                                                                                                                          >  1:989316/6‑150 (MQ=255)
            cAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGGGAGAACGGGAGGCCGGGTCACCAAAGGGTCCGTCCATCGTCCTAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCa                                                                                                                                         <  2:677039/150‑1 (MQ=255)
                gcCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCgg                                                                                                                                     >  1:423900/1‑150 (MQ=255)
                 ccGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGc                                                                                                                                    >  1:1590426/1‑150 (MQ=255)
                    aCCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTg                                                                                                                                 <  2:924215/150‑1 (MQ=255)
                      cAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGt                                                                                                                               <  1:1190127/150‑1 (MQ=255)
                        gATCTGCAATCTGATTACGCGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCCGGTGGACCCGCGTgttgc                                                                                                                             <  1:731627/150‑5 (MQ=255)
                          tCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCCGGTGGACCCGCGTgttgccg                                                                                                                           <  1:104273/150‑7 (MQ=255)
                          tCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGTgttgcc                                                                                                                            >  2:204929/1‑145 (MQ=255)
                            ggCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGCAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGa                                                                                                                         >  2:1552829/1‑150 (MQ=255)
                            ggCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGa                                                                                                                         <  1:17871/150‑1 (MQ=255)
                            ggCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGTgttgccga                                                                                                                          <  1:678368/150‑8 (MQ=38)
                             gCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACTGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGag                                                                                                                        <  1:760896/150‑1 (MQ=255)
                             gCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGag                                                                                                                        <  1:160087/150‑1 (MQ=255)
                              cAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGagt                                                                                                                       <  1:773578/150‑1 (MQ=255)
                                aTCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGT‑GTTGCCGagaaa                                                                                                                     <  1:204929/150‑4 (MQ=37)
                                 tCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGT‑GTTGCCGagaaaa                                                                                                                    <  1:730014/150‑5 (MQ=37)
                                  cTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTc                                                                                                                   <  1:271923/150‑1 (MQ=255)
                                   tGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCg                                                                                                                  >  2:1508031/1‑150 (MQ=255)
                                   tGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCg                                                                                                                  >  2:212149/1‑150 (MQ=255)
                                   tGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCg                                                                                                                  <  2:195817/150‑1 (MQ=255)
                                       tACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGAt                                                                                                              >  1:1033729/1‑150 (MQ=255)
                                        aCGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATa                                                                                                             >  1:306774/1‑150 (MQ=255)
                                            gCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATc                                                                                                         <  1:1430331/150‑1 (MQ=255)
                                              gATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCgg                                                                                                       <  2:1271872/150‑1 (MQ=255)
                                                tatCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTa                                                                                                     >  1:1418135/1‑150 (MQ=255)
                                                 atCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACTGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTaa                                                                                                    <  1:1421252/150‑1 (MQ=255)
                                                  tCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAt                                                                                                   >  1:1225428/1‑150 (MQ=255)
                                                    tACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                                                                                                 >  1:226813/1‑150 (MQ=255)
                                                     aCCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTc                                                                                                <  2:306774/150‑1 (MQ=255)
                                                     aCCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTc                                                                                                >  2:1194213/1‑150 (MQ=255)
                                                       cGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCAt                                                                                              <  2:1444600/150‑1 (MQ=255)
                                                        gCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCAtt                                                                                             >  2:1006613/1‑150 (MQ=255)
                                                          ttcttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGc                                                                                           <  2:500935/150‑1 (MQ=255)
                                                            cttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc                                                                                         <  2:1418135/150‑1 (MQ=255)
                                                            cttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc                                                                                         <  1:298328/150‑1 (MQ=255)
                                                            cttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc                                                                                         <  1:1006613/150‑1 (MQ=255)
                                                             ttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctct                                                                                        <  2:1004501/150‑1 (MQ=255)
                                                              tcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCta                                                                                       >  2:151244/1‑150 (MQ=255)
                                                                 gCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaa                                                                                    <  2:865838/150‑1 (MQ=255)
                                                                    tGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACt                                                                                 <  2:556966/150‑1 (MQ=255)
                                                                     gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaa                                                                                    <  1:1433426/146‑1 (MQ=255)
                                                                     gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaa                                                                                    >  2:1433426/1‑146 (MQ=255)
                                                                     gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTc                                                                                >  2:1482125/1‑150 (MQ=255)
                                                                     gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTc                                                                                <  1:957305/150‑1 (MQ=255)
                                                                       cAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTGATTTCATTGCTCTATAAACTCCg                                                                              >  1:388041/1‑150 (MQ=255)
                                                                       cAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCg                                                                              >  2:968330/1‑150 (MQ=255)
                                                                         gCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTa                                                                            <  1:1601209/150‑1 (MQ=255)
                                                                            gAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACAt                                                                         <  1:787839/150‑1 (MQ=255)
                                                                            gAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACAt                                                                         >  2:787839/1‑150 (MQ=255)
                                                                               aCGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCAc                                                                      >  1:1118553/1‑150 (MQ=255)
                                                                                  gTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCa                                                                   >  2:184367/1‑150 (MQ=255)
                                                                                    gagaACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAAt                                                                 >  1:1080792/1‑150 (MQ=255)
                                                                                     agaACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATg                                                                >  2:74031/1‑150 (MQ=255)
                                                                                       aaCGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCa                                                              <  1:1552829/150‑1 (MQ=255)
                                                                                         cgtgAGGCCGGGTTACCAAAGGTTCCTTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTTGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTATATAAACTCCGTACATCACTCAATGCAAg                                                            <  1:1636164/147‑1 (MQ=255)
                                                                                         cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAg                                                            <  2:719462/150‑1 (MQ=255)
                                                                                           ggAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGa                                                          >  2:1222276/1‑150 (MQ=255)
                                                                                              ggCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATc                                                       <  2:1406872/150‑1 (MQ=255)
                                                                                               gCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCa                                                      <  2:585845/150‑1 (MQ=255)
                                                                                                  gggTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGc                                                   <  1:1482125/150‑1 (MQ=255)
                                                                                                  gggTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGc                                                   <  2:423900/150‑1 (MQ=255)
                                                                                                      tACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTAcc                                               >  1:1141904/1‑150 (MQ=255)
                                                                                                       aCCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCg                                              >  1:1393283/1‑150 (MQ=255)
                                                                                                        ccAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCgg                                             >  2:448872/1‑150 (MQ=255)
                                                                                                           aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTg                                          <  2:1225428/150‑1 (MQ=255)
                                                                                                             ggTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGa                                        <  1:18169/150‑1 (MQ=255)
                                                                                                             ggTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGa                                        <  1:1508031/150‑1 (MQ=255)
                                                                                                               ttCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCTGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATg                                      <  1:1377931/150‑1 (MQ=255)
                                                                                                                  cGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTAc                                   >  1:845655/1‑150 (MQ=255)
                                                                                                                    tCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGa                                 >  2:1579761/1‑150 (MQ=255)
                                                                                                                        tCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCgt                             >  2:1559374/1‑150 (MQ=255)
                                                                                                                           tCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGtt                          >  1:355870/1‑150 (MQ=255)
                                                                                                                             aTAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTa                        <  2:989316/150‑1 (MQ=255)
                                                                                                                               aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACg                      <  2:1219814/150‑1 (MQ=255)
                                                                                                                                    gCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTa                 >  2:597454/1‑150 (MQ=255)
                                                                                                                                      atcatTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTAtt               <  1:968330/150‑1 (MQ=255)
                                                                                                                                      atcatTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTAtt               >  1:1237504/1‑150 (MQ=255)
                                                                                                                                         atTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAg            <  1:312763/150‑1 (MQ=255)
                                                                                                                                             tctcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACt        >  1:868292/1‑150 (MQ=255)
                                                                                                                                              ctcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTc       >  2:617687/1‑150 (MQ=255)
                                                                                                                                              ctcCGCAACCACGCCGGTGGACCCGCGT‑GTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTc       >  1:542129/1‑150 (MQ=255)
                                                                                                                                               tcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTcc      >  2:339358/1‑150 (MQ=255)
                                                                                                                                                 ggCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCgg    >  1:1460027/1‑150 (MQ=255)
                                                                                                                                                  gCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGc   >  2:915660/1‑150 (MQ=255)
                                                                                                                                                  gCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTA‑TTAGCACTCCGGcc  >  1:1174881/1‑150 (MQ=255)
                                                                                                                                                   cAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGcc  >  2:137532/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TTTCACGCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCC  >  NC_000913/2661327‑2661623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑