Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,383,867 T→C 5.2% L10P (CTG→CCG) ‡ yjeM → putative transporter YjeM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,383,8670TC 5.2% 421.8 / 8.4 136L10P (CTG→CCG) ‡yjeMputative transporter YjeM
Reads supporting (aligned to +/- strand):  ref base T (79/49);  new base C (3/4);  total (83/53)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 138 total aligned reads displayed.

GATATTTGATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGA  >  NC_000913/4383721‑4384014
                                                                                                                                                  |                                                                                                                                                   
gATATTTGATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGAt                                                                                                                                                  >  2:921965/1‑150 (MQ=255)
gATATTTGATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGAt                                                                                                                                                  <  1:672339/150‑1 (MQ=255)
    tttGATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGa                                                                                                                                              >  1:1154054/1‑150 (MQ=255)
     ttGATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGAc                                                                                                                                             >  1:346844/1‑150 (MQ=255)
      tGATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACt                                                                                                                                            >  1:1016166/1‑150 (MQ=255)
       gATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTc                                                                                                                                           <  2:382345/150‑1 (MQ=255)
        atatCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCa                                                                                                                                          <  2:1440175/150‑1 (MQ=255)
         tatCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCat                                                                                                                                         >  2:573953/1‑150 (MQ=255)
          atcatcCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCata                                                                                                                                        >  2:556963/1‑150 (MQ=255)
           tcatcCAGGTATCAATTCTGTATTGGTTTTTACGCCGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCCTATCAGACTCatct                                                                                                                                       <  1:1414219/150‑3 (MQ=255)
            catcCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATAtt                                                                                                                                      <  2:1624185/150‑1 (MQ=255)
             atcCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATtg                                                                                                                                     >  2:463176/1‑150 (MQ=255)
               ccAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATtgat                                                                                                                                   >  2:28169/1‑150 (MQ=255)
               ccAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATtgat                                                                                                                                   >  2:1203880/1‑150 (MQ=255)
               ccAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCCTATCAGACTCATCttttt                                                                                                                                   <  1:669084/150‑4 (MQ=255)
                cAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATtgatg                                                                                                                                  >  1:964756/1‑150 (MQ=255)
                cAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGCTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATtgatg                                                                                                                                  <  1:498325/150‑1 (MQ=255)
                 aGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATtgatga                                                                                                                                 >  2:980932/1‑150 (MQ=255)
                  ggTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCCTATCAGACTCATCttttttat                                                                                                                                <  1:373880/150‑7 (MQ=255)
                     aTCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCtt                                                                                                                             >  1:1143022/1‑150 (MQ=255)
                      tCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCTGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCttt                                                                                                                            <  2:688572/150‑1 (MQ=255)
                       cAATTCTGTATTGGGTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTAAGTGGGCACCTGTAATACCCGCGCTGAAATCAAGGATGGTTTCATGCCTCAAACGAAAAAAAAGATGAGTCAGATAGGACTCATATTGATGATCTTTa                                                                                                                           >  2:1002715/1‑150 (MQ=255)
                        aaTTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTAc                                                                                                                          >  2:725448/1‑150 (MQ=255)
                             tGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCg                                                                                                                     <  1:1024954/150‑1 (MQ=255)
                                     tttttACGCTGTCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGa                                                                                                             <  2:1630544/150‑1 (MQ=255)
                                     tttttACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGa                                                                                                             >  1:494840/1‑150 (MQ=255)
                                       tttACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGAtt                                                                                                           <  1:1477957/150‑1 (MQ=255)
                                         tACGCTGCCTGCTCAATGTTGCTCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTg                                                                                                         <  2:326839/150‑1 (MQ=255)
                                         tACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCATTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTg                                                                                                         <  2:1318112/150‑1 (MQ=255)
                                         tACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTg                                                                                                         <  1:152153/150‑1 (MQ=255)
                                         tACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTg                                                                                                         <  1:158182/150‑1 (MQ=255)
                                              tgactgcTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGACTGATAGGACTCATATTGATGATCTTTACTTCCGTATTAGGATTTGCCaaa                                                                                                    >  2:1083453/4‑149 (MQ=255)
                                                 ctgcTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGc                                                                                                 >  1:1275993/1‑150 (MQ=255)
                                                  tgcTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGcc                                                                                                <  1:921965/150‑1 (MQ=255)
                                                   gcTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGccc                                                                                               <  1:1058219/150‑1 (MQ=255)
                                                   gcTCAATGTTGCACAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGccc                                                                                               >  2:452967/1‑150 (MQ=255)
                                                      cAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATc                                                                                            >  2:833601/1‑150 (MQ=255)
                                                        aTGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCgg                                                                                          >  2:738042/1‑150 (MQ=255)
                                                        aTGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCgg                                                                                          <  2:867543/150‑1 (MQ=255)
                                                         tGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTa                                                                                                                   <  2:1108473/124‑1 (MQ=255)
                                                          gTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCt                                                                                        >  1:1259514/1‑150 (MQ=255)
                                                           ttGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCtt                                                                                       <  1:1596498/150‑1 (MQ=255)
                                                           ttGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCtt                                                                                       >  1:785192/1‑150 (MQ=255)
                                                            tGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCtta                                                                                      >  2:124684/1‑150 (MQ=255)
                                                             gcgcAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCttat                                                                                     >  2:501181/1‑150 (MQ=255)
                                                              cgaagtccactTCAATGGTCACGTTTTATAAAAGAGATGAAATCAATGATCGTTTAATGCATCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCttatt                                                                                    >  2:1279956/4‑150 (MQ=255)
                                                               gcagtgcagtTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCttatta                                                                                   >  1:1055996/1‑150 (MQ=255)
                                                                cagtgcagtTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTAc                                                                                  <  1:417451/150‑1 (MQ=255)
                                                                   tgcagtTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTa                                                                               <  1:519203/150‑1 (MQ=255)
                                                                       gtTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATgg                                                                           <  1:28169/150‑1 (MQ=255)
                                                                       gtTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATAGGCTTATTACTTAATgg                                                                           >  1:135072/1‑150 (MQ=255)
                                                                         tCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGt                                                                         >  2:858566/1‑150 (MQ=255)
                                                                          cAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGtt                                                                        <  1:573953/150‑1 (MQ=255)
                                                                          cAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGtt                                                                        >  1:461656/1‑150 (MQ=255)
                                                                           aGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTta                                                                       >  2:674887/1‑150 (MQ=255)
                                                                              gggCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAg                                                                    >  1:661060/1‑150 (MQ=255)
                                                                               ggCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGt                                                                   >  2:951714/1‑150 (MQ=255)
                                                                                 cACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGc                                                                 <  2:1069869/150‑1 (MQ=255)
                                                                                    gtgtTATACACGCTCTGAAATCAACGATGGTTTCATGCCTCACAAGATAAAAAAGAAGAGTCTGATAGGACTCATATTGATTATCTTTACTTCCGTATTTGGATTTGCCAATATACCATCCGCTTATTACTTAATGTCTTATAGTGCGAt                                                              >  2:533740/1‑150 (MQ=255)
                                                                                    gtgtTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGAt                                                              >  2:1214427/1‑150 (MQ=255)
                                                                                     tgtTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGAtt                                                             >  1:813491/1‑150 (MQ=255)
                                                                                      gtTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTc                                                            >  2:1493011/1‑150 (MQ=255)
                                                                                         ataCACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATTAGTCTGATAGGACTCATATTGATGATCTTTAATTCCGTATTTGGATTTGCCATTATCCCATAGGCTTATTACTTAATGGGTTATAGTTCGATTCCCt                                                         <  2:199042/150‑1 (MQ=255)
                                                                                          taCACGCGCTGAGATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCtt                                                        >  2:447779/1‑150 (MQ=255)
                                                                                           acacGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCttt                                                       >  1:106529/1‑150 (MQ=255)
                                                                                            cacGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGAt                                                              >  2:810643/1‑142 (MQ=255)
                                                                                            cacGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGAt                                                              <  1:810643/142‑1 (MQ=255)
                                                                                            cacGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCtttt                                                      >  1:1536682/1‑150 (MQ=255)
                                                                                             ccgcgCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTta                                                     <  2:1580249/149‑1 (MQ=255)
                                                                                              cgcgCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTtat                                                    >  2:933711/1‑150 (MQ=255)
                                                                                                   tGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATAtt                                               <  2:227945/150‑1 (MQ=255)
                                                                                                    gAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATAttt                                              >  2:1332186/1‑150 (MQ=255)
                                                                                                     aaaTGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATAtttt                                             >  1:1412126/1‑150 (MQ=255)
                                                                                                       aTGAAGGATTGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATATGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCt                                           <  1:1215912/150‑1 (MQ=255)
                                                                                                        tGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTg                                          >  2:1451844/1‑150 (MQ=255)
                                                                                                         gAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGc                                         >  1:1474210/1‑150 (MQ=255)
                                                                                                           aGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCAt                                       <  1:501181/150‑1 (MQ=255)
                                                                                                             gATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTg                                     >  1:582108/1‑150 (MQ=255)
                                                                                                                ggTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTa                                  >  2:1208069/1‑150 (MQ=255)
                                                                                                                          cTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTc                        <  2:1382207/150‑1 (MQ=255)
                                                                                                                            cacaCGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCa                      >  2:194803/1‑150 (MQ=255)
                                                                                                                              cacGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGATTATTACTAAATGGGTTATAGTGAGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCAtt                    >  2:212053/1‑150 (MQ=255)
                                                                                                                                cGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTATTTATTCCATTCg                  >  2:1670654/1‑150 (MQ=255)
                                                                                                                                  aTAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGcc                >  1:27492/1‑150 (MQ=255)
                                                                                                                                   tAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGGATTGTTATTCTTTATTCAATTCGCCt               >  1:455193/1‑150 (MQ=255)
                                                                                                                                    aaaaaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGcc                >  2:505323/1‑148 (MQ=255)
                                                                                                                                    aaaaaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCtt              <  2:964756/150‑1 (MQ=255)
                                                                                                                                      aaaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTaa            <  2:379301/150‑1 (MQ=255)
                                                                                                                                      aaaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGATTATTAATTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTaa            >  1:1019593/1‑150 (MQ=255)
                                                                                                                                       aaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAat           <  2:1477781/150‑1 (MQ=255)
                                                                                                                                        aataTGAGTCCTATCAGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAatg          >  1:251575/4‑150 (MQ=255)
                                                                                                                                        aataTGAGTCCTATCAGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAatg          >  1:537306/4‑150 (MQ=255)
                                                                                                                                          gATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAatgat        >  1:157776/1‑150 (MQ=255)
                                                                                                                                            tGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATgg      <  2:1497488/150‑1 (MQ=255)
                                                                                                                                              aGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCt    <  2:346844/150‑1 (MQ=255)
                                                                                                                                              aGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCt    <  1:933711/150‑1 (MQ=255)
                                                                                                                                                tCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGa  <  1:1564807/150‑1 (MQ=255)
                                                                                                                                                tCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGa  <  1:1203880/150‑1 (MQ=255)
                                                                                                                                                tCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGa  <  2:1016166/150‑1 (MQ=255)
                                                                                                                                                  tGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCa                      >  1:1173288/1‑128 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATATTTGATATCATCCAGGTATCAATTCTGTATTGGTTTTTACGCTGCCTGCTCAATGTTGCGCAGTGCAGTTCAGTGGGCACGTGTTATACACGCGCTGAAATGAAGGATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGA  >  NC_000913/4383721‑4384014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑