Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,400,322 T→C 5.3% L12P (CTG→CCG)  hfq → RNA‑binding protein Hfq

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,400,3220TC 5.3% 301.2 / 4.8 96L12P (CTG→CCG) hfqRNA‑binding protein Hfq
Reads supporting (aligned to +/- strand):  ref base T (42/48);  new base C (2/3);  total (45/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTG  >  NC_000913/4400174‑4400471
                                                                                                                                                    |                                                                                                                                                      
tACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTg                                                                                                                                                       <  1:112494/150‑1 (MQ=255)
    ggTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGaaag                                                                                                                                                   >  1:1381458/1‑148 (MQ=255)
        gttgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACt                                                                                                                                               >  2:1135912/1‑150 (MQ=255)
          tgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGc                                                                                                                                             <  2:403283/150‑1 (MQ=255)
           ggTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCg                                                                                                                                            >  2:1613821/1‑150 (MQ=255)
             ggCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑Tc                                                                                                                                         <  2:1489257/149‑1 (MQ=255)
              gCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCg                                                                                                                                        >  2:1306966/1‑150 (MQ=255)
              gCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCg                                                                                                                                        >  2:985734/1‑150 (MQ=255)
               cTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCgg                                                                                                                                       <  1:1184522/150‑1 (MQ=255)
                tATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCggg                                                                                                                                      <  1:985734/150‑1 (MQ=255)
                 aTCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGa                                                                                                                                     <  1:1613821/150‑1 (MQ=255)
                    gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACg                                                                                                                                  >  1:865481/1‑150 (MQ=255)
                    gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCCG‑ACGCAGTGCGTTCAGGAACg                                                                                                                                  <  2:758677/150‑1 (MQ=255)
                    gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCCG‑ACGCAGTGCGTTCAGGAACg                                                                                                                                  <  1:2131/150‑1 (MQ=255)
                       ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAATAGATAATGGATAAGGGGAAATCTTTACAAGATACGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                               <  2:1309175/150‑1 (MQ=255)
                       ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                               <  2:506732/150‑1 (MQ=255)
                       ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                               <  1:1345594/150‑1 (MQ=255)
                        gCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                               >  1:909216/1‑149 (MQ=255)
                        gCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                               <  2:909216/149‑1 (MQ=255)
                        gCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGtt                                                                                                                              <  1:1545008/150‑1 (MQ=255)
                          tGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTcc                                                                                                                            <  1:1523169/150‑1 (MQ=255)
                              tgtgTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGtt                                                                                                                        >  2:1851/1‑150 (MQ=255)
                              tgtgTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGtt                                                                                                                        <  1:429531/150‑1 (MQ=255)
                               gtgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGttt                                                                                                                       <  1:780222/150‑1 (MQ=255)
                                tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                      >  1:1533464/1‑150 (MQ=255)
                                tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                      <  1:269979/150‑1 (MQ=255)
                                tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                      <  2:1182781/150‑1 (MQ=255)
                                tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                      >  1:86492/1‑150 (MQ=255)
                                   aCAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtat                                                                                                                   >  2:3772/1‑150 (MQ=255)
                                    cAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCAGAACGGACTGCG‑TCGGGAACGTGTTCCAGTTTCtatt                                                                                                                  >  2:559198/1‑150 (MQ=255)
                                     aaTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtattt                                                                                                                 <  2:456085/150‑1 (MQ=255)
                                        tGAGACGTATCTTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtatttatt                                                                                                              <  2:158302/150‑1 (MQ=255)
                                           gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTATatttattttg                                                                                                           >  2:1560426/1‑148 (MQ=255)
                                             cGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                                                                                         <  2:1344192/150‑1 (MQ=255)
                                             cGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                                                                                         >  2:659983/1‑150 (MQ=255)
                                                aTCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAAt                                                                                                      <  1:1142104/150‑1 (MQ=255)
                                                 tCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                                                                                     <  2:1296657/150‑1 (MQ=255)
                                                 tCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                                                                                     <  2:1302693/150‑1 (MQ=255)
                                                      cgcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAtt                                                                                                >  1:1437766/1‑150 (MQ=255)
                                                        cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                                                                              <  1:1450499/150‑1 (MQ=255)
                                                            tttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTg                                                                                          <  1:432267/150‑1 (MQ=255)
                                                             ttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGc                                                                                         <  1:292205/150‑1 (MQ=255)
                                                                 cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                                                                                     <  1:757085/150‑1 (MQ=255)
                                                                 cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                                                                                     <  1:489271/150‑1 (MQ=255)
                                                                   gAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGTAAAAGAGATAATGGCTAAGGGGCAATCTTTACAAGATTCGTTCCTGTACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAATCTTCAAggtc                                                                                   <  2:1446239/150‑3 (MQ=255)
                                                                    aaTCGAAAGATTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGCGCAATCTTAACAAGATCCGTTCCTGAACGCACTGCG‑TCTGGAAAGTGTTCCAGTTTCTATTTATTTGGTGAATGTTATTAAGCTGCAAgggaa                                                                                  >  2:1351930/1‑148 (MQ=255)
                                                                        gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc                                                                              >  2:1011558/1‑150 (MQ=255)
                                                                         aaaGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCg                                                                             <  2:1437766/150‑1 (MQ=255)
                                                                           tggTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGGTATGGGGCAATCTTTAGTAGATCCGTTCCTGAACGGACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTTTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg                                                                           <  2:1381458/149‑1 (MQ=255)
                                                                             gTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTc                                                                         >  2:201639/1‑150 (MQ=255)
                                                                             gTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTc                                                                         >  1:794063/1‑150 (MQ=255)
                                                                              ttCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCt                                                                        >  1:1128036/1‑150 (MQ=255)
                                                                               tCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtt                                                                       >  1:450642/1‑150 (MQ=255)
                                                                                 aaaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt                                                                     <  2:794063/150‑1 (MQ=255)
                                                                                 aaaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt                                                                     >  2:188300/1‑150 (MQ=255)
                                                                                  aaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTg                                                                    <  2:428929/150‑1 (MQ=255)
                                                                                   aGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGa                                                                   <  1:1414649/150‑1 (MQ=255)
                                                                                         aaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTg                                                                    >  1:67526/1‑143 (MQ=255)
                                                                                         aaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTg                                                                    <  2:67526/143‑1 (MQ=255)
                                                                                             aGCATATACGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTg                                                         >  1:371687/1‑150 (MQ=255)
                                                                                              gCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGa                                                        >  1:1216030/1‑150 (MQ=255)
                                                                                                atatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATc                                                      >  1:1204130/1‑150 (MQ=255)
                                                                                                atatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCACTTCGTGATc                                                      >  2:149936/1‑150 (MQ=255)
                                                                                                 tatgAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                     >  2:397446/1‑150 (MQ=255)
                                                                                                 tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                     >  1:1306710/1‑150 (MQ=255)
                                                                                                    aaGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGt                                                  <  1:659983/150‑1 (MQ=255)
                                                                                                     aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                 >  2:549857/1‑150 (MQ=255)
                                                                                                     aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                 <  1:520857/150‑1 (MQ=255)
                                                                                                     aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                 >  2:1603172/1‑150 (MQ=255)
                                                                                                     aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                 >  2:1536092/1‑150 (MQ=255)
                                                                                                      ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                >  1:1140446/1‑150 (MQ=255)
                                                                                                      ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                <  2:1306710/150‑1 (MQ=255)
                                                                                                      ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                <  1:3772/150‑1 (MQ=255)
                                                                                                          aaGAGAGAATGGCTATGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaa                                            >  1:332540/1‑150 (MQ=255)
                                                                                                           agagagAATGGCTAAGGGGCTATCTTTACAAGATCCGTTCCTCAAGTCACTGCG‑TCGTGAACGTGTTCCAGCTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGCCAAATCGAGTCTTTTGATCTTTTCGTGATCCTGTTGaaaaa                                           <  2:466236/150‑1 (MQ=255)
                                                                                                                      cTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                <  1:201639/150‑1 (MQ=255)
                                                                                                                             gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGt                         <  1:772595/150‑1 (MQ=255)
                                                                                                                               aaTCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGttt                       >  2:1606872/1‑150 (MQ=255)
                                                                                                                                  cTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                    <  1:541452/150‑1 (MQ=255)
                                                                                                                                   tttACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACaa                   >  1:1229803/1‑150 (MQ=255)
                                                                                                                                    ttACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAg                  <  2:86492/150‑1 (MQ=255)
                                                                                                                                     tACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                 >  1:751042/1‑150 (MQ=255)
                                                                                                                                      aCAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCa                >  1:490431/1‑150 (MQ=255)
                                                                                                                                       gaaGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTAGTTATTTGGTGAATGGTATTAAGCTGCCAGGGCAAATCGAGTCTTTTGATCAGGTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGccc               >  1:1335769/2‑148 (MQ=255)
                                                                                                                                        ggaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg              >  2:992749/7‑150 (MQ=255)
                                                                                                                                          gATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgcg            <  1:1536092/150‑1 (MQ=255)
                                                                                                                                             acGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                >  1:747936/2‑111 (MQ=38)
                                                                                                                                             acGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                <  2:747936/110‑1 (MQ=38)
                                                                                                                                              cGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAttt        >  2:85466/1‑150 (MQ=255)
                                                                                                                                              cGTTCCTGAACGCACTCCG‑TCGTGAACGTGTTCCAGTTTCAATTTATTTGGTGAATGGTATAAAGCGGCAACGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAttt        <  2:894796/150‑1 (MQ=255)
                                                                                                                                               gTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc       <  1:1135912/150‑1 (MQ=255)
                                                                                                                                               gTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc       <  1:107120/150‑1 (MQ=255)
                                                                                                                                                  ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAACAACAAGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc    >  2:1444148/1‑150 (MQ=255)
                                                                                                                                                  ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc       >  2:1519281/1‑147 (MQ=255)
                                                                                                                                                  ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc       <  1:1519281/147‑1 (MQ=255)
                                                                                                                                                  ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc    >  2:1397949/1‑150 (MQ=255)
                                                                                                                                                    tGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtg  <  1:1257239/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTG  >  NC_000913/4400174‑4400471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑