Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,620,096 A→T 5.1% K165M (AAG→ATG) ‡ deoB → phosphopentomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,620,0960AT 5.1% 301.3 / 2.9 92K165M (AAG→ATG) ‡deoBphosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (41/46);  new base T (3/2);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCT  >  NC_000913/4619949‑4620241
                                                                                                                                                   |                                                                                                                                                 
atTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGc                                                                                                                                                 >  2:530469/1‑150 (MQ=255)
       cGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGAtttt                                                                                                                                          >  1:465800/1‑150 (MQ=255)
        gATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTc                                                                                                                                         <  2:254966/150‑1 (MQ=255)
        gATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTc                                                                                                                                         >  1:1191911/1‑150 (MQ=255)
         aTCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCt                                                                                                                                        <  1:906314/150‑1 (MQ=255)
          tCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCta                                                                                                                                       <  1:778461/150‑1 (MQ=255)
           cACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAATCGGCTTGCCGGTTTTcatg                                                                                                                                      <  1:1097443/150‑4 (MQ=255)
           cACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCtat                                                                                                                                      <  1:267273/150‑1 (MQ=255)
            aCGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCtata                                                                                                                                     >  1:162054/1‑150 (MQ=255)
               aaaaCAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCt                                                                                                                                  >  2:812979/1‑150 (MQ=255)
                   cAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAATCGGCTTGCCGGTTTTcatgt                                                                                                                                     >  2:490569/1‑139 (MQ=255)
                   cAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAATCGGCTTGCCGGTTTTcatgt                                                                                                                                     <  1:490569/143‑5 (MQ=255)
                   cAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGc                                                                                                                              <  2:86697/150‑1 (MQ=255)
                     gCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCGCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTg                                                                                                                            <  2:589064/150‑1 (MQ=255)
                         cccGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTc                                                                                                                        <  1:1200633/150‑1 (MQ=255)
                         cccGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTc                                                                                                                        <  1:125930/150‑1 (MQ=255)
                            gCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCgt                                                                                                                     >  1:792721/1‑150 (MQ=255)
                            gCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCgt                                                                                                                     >  1:50884/1‑150 (MQ=255)
                             cAAGAGCTGCTGGATTAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCgtg                                                                                                                    <  2:1593727/150‑1 (MQ=255)
                               agagCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGtt                                                                                                                  <  1:568367/150‑1 (MQ=255)
                                 agCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTcc                                                                                                                <  2:1023452/150‑1 (MQ=255)
                                    tgctgTATAAACTGGTCGAACGCGCTTATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGa                                                                                                             <  1:1462514/150‑1 (MQ=255)
                                       tgGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtg                                                                                                          <  1:670118/150‑1 (MQ=255)
                                        ggATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgc                                                                                                         >  2:1635093/1‑150 (MQ=255)
                                        ggATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgc                                                                                                         >  2:1294772/1‑150 (MQ=255)
                                        ggATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgc                                                                                                         >  1:1166357/1‑150 (MQ=255)
                                            aaaCTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgcctgc                                                                                                     <  2:1075982/150‑1 (MQ=255)
                                             aaCTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCAGCTGACTCCGTGTTCCAGATtgcctgcc                                                                                                    <  2:1406245/150‑1 (MQ=255)
                                             aaCTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCAGCTGACTCCGTGTTCCAGATtgcctgcc                                                                                                    >  1:1406245/1‑150 (MQ=255)
                                              aCTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCa                                                                                                   >  2:1459556/1‑150 (MQ=255)
                                               cTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCAt                                                                                                  <  2:384512/150‑1 (MQ=255)
                                                tGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATg                                                                                                 <  2:162054/150‑1 (MQ=255)
                                                           gcTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTc                                                                                      <  1:1126259/150‑1 (MQ=255)
                                                            cTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCAGGAAGAAAGTTGCg                                                                                     >  1:558277/1‑150 (MQ=255)
                                                               aTCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTc                                                                                  <  1:479365/150‑1 (MQ=255)
                                                                 cTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATGCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACGCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTg                                                                                <  2:98233/150‑1 (MQ=255)
                                                                  tGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTgg                                                                               <  2:820692/150‑1 (MQ=255)
                                                                   gCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGa                                                                              >  2:1294878/1‑150 (MQ=255)
                                                                   gCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGa                                                                              <  1:853461/150‑1 (MQ=255)
                                                                   gCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGa                                                                              >  1:868581/1‑150 (MQ=255)
                                                                     cGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATa                                                                            >  1:1428353/1‑150 (MQ=255)
                                                                            ccTCGGTAACTGCCACTCTTCCGGTACTGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTa                                                                     <  2:1596351/150‑1 (MQ=255)
                                                                            ccTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAActc                                                                       >  2:206038/1‑148 (MQ=255)
                                                                            ccTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAActc                                                                       <  1:206038/148‑1 (MQ=255)
                                                                            ccTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTa                                                                     <  1:721224/150‑1 (MQ=255)
                                                                            ccTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTa                                                                     <  2:1166357/150‑1 (MQ=255)
                                                                             cTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTAc                                                                    <  1:1450646/150‑1 (MQ=255)
                                                                             cTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTAc                                                                    <  2:1191911/150‑1 (MQ=255)
                                                                              tCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACg                                                                   <  2:1189140/150‑1 (MQ=255)
                                                                               cGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGa                                                                  >  2:1272065/1‑150 (MQ=255)
                                                                                  tAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACt                                                               >  1:128800/1‑150 (MQ=255)
                                                                                       gCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCgg                                                                                    >  2:1037033/1‑124 (MQ=255)
                                                                                       gCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCgg                                                                                    <  1:1037033/124‑1 (MQ=255)
                                                                                       gCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCg                                                          >  1:681242/1‑150 (MQ=255)
                                                                                        ccACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGa                                                         <  2:1640474/150‑1 (MQ=255)
                                                                                               tCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGcc                                                  >  2:1552310/1‑150 (MQ=255)
                                                                                               tCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGcc                                                  >  1:41045/1‑150 (MQ=255)
                                                                                                   gTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTg                                              <  2:50884/150‑1 (MQ=255)
                                                                                                       ggTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAaga                                          >  2:897968/1‑150 (MQ=255)
                                                                                                        gTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAagag                                         >  1:1398374/1‑150 (MQ=255)
                                                                                                          cATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCt                                       <  2:508105/150‑1 (MQ=255)
                                                                                                          cATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCt                                       >  1:682113/1‑150 (MQ=255)
                                                                                                           aTTCTCGATCAACTGGGCGAAGAGCAAATGAAAACAGGCAAGCCGATTTTCTATACATCCGATGACAACGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTg                                      >  2:1587700/1‑150 (MQ=255)
                                                                                                              cTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGAcc                                   >  1:204633/1‑150 (MQ=255)
                                                                                                                  aTCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAAcg                               >  1:480813/1‑150 (MQ=255)
                                                                                                                     aaCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAAcggcg                            >  1:1196038/1‑150 (MQ=255)
                                                                                                                     aaCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAAcggcg                            >  2:1153735/1‑150 (MQ=255)
                                                                                                                        tGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCt                         >  1:579442/1‑150 (MQ=255)
                                                                                                                         gggCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCTCCCGTGAAGAGCTGACCAACGGCGGCTa                        >  1:1464852/1‑150 (MQ=255)
                                                                                                                           gCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACa                      <  1:1178223/150‑1 (MQ=255)
                                                                                                                           gCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACa                      >  1:758173/1‑150 (MQ=255)
                                                                                                                               agagCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTg                                      >  1:1474377/1‑130 (MQ=255)
                                                                                                                               agagCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTg                                      <  2:1474377/130‑1 (MQ=255)
                                                                                                                                cagCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATc                 <  1:1606479/149‑1 (MQ=255)
                                                                                                                                  gCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCgg               <  1:1187168/150‑1 (MQ=255)
                                                                                                                                  gCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCgg               <  1:530469/150‑1 (MQ=255)
                                                                                                                                     caTGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCg            >  2:983569/1‑150 (MQ=255)
                                                                                                                                      aTGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCgt           >  2:1248307/1‑150 (MQ=255)
                                                                                                                                       tGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACtgtg                                                            <  2:817817/100‑1 (MQ=255)
                                                                                                                                       tGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACtgtg                                                            >  1:949977/1‑100 (MQ=255)
                                                                                                                                       tGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACtgtg                                                            <  2:949977/100‑1 (MQ=255)
                                                                                                                                       tGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACtgtg                                                            >  1:817817/1‑100 (MQ=255)
                                                                                                                                       tGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCgtg          >  1:1653386/1‑150 (MQ=255)
                                                                                                                                       tGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCgtg          >  2:7213/1‑150 (MQ=255)
                                                                                                                                       tGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCgtg          <  2:758173/150‑1 (MQ=255)
                                                                                                                                       agAAAATCGGCTTGCCGGTTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCgtg          >  1:412563/2‑150 (MQ=255)
                                                                                                                                       agAAAATCGGCTTGCCGGTTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCgtg          >  1:812280/2‑150 (MQ=255)
                                                                                                                                         aaaaCCCGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGtt        <  1:1431532/150‑1 (MQ=255)
                                                                                                                                            aCCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATc     <  2:681242/150‑1 (MQ=255)
                                                                                                                                             ccGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGATATCg    <  1:897968/150‑1 (MQ=255)
                                                                                                                                               ggCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTGCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGTATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGTCTGCTACAATATCGGTCGTGTTATCGCt  <  2:792721/150‑1 (MQ=255)
                                                                                                                                               ggCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCt  <  2:868581/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCT  >  NC_000913/4619949‑4620241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑