BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,146,736	A→G	7.9%	noncoding (148/169 nt)	sraB →	small RNA SraB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,736	0	A	G	7.9%	327.9 / 14.9	114	noncoding (148/169 nt)	sraB	small RNA SraB
Reads supporting (aligned to +/- strand): ref base A (48/57); new base G (4/5); total (52/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

Only 100 of 117 total aligned reads displayed.

TTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGT  >  NC_000913/1146588‑1146885

ttAGGCATATTTTTTTCCATCAGATATAGTGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAAc                                                                                                                                                      <  2:941449/150‑1 (MQ=255)

   ggCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACcac                                                                                                                                                   >  1:1042506/1‑150 (MQ=255)

    gCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACcaca                                                                                                                                                  <  2:1320367/150‑1 (MQ=255)

     cATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAAc                                                                                                                                                      >  2:1652832/1‑145 (MQ=255)

     cATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAAc                                                                                                                                                      <  1:1652832/145‑1 (MQ=255)

     cATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAg                                                                                                                                                 >  1:932570/1‑150 (MQ=255)

      atatTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAgg                                                                                                                                                >  1:460936/1‑150 (MQ=255)

               tCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCtttt                                                                                                                                       <  2:470022/150‑1 (MQ=255)

                ccATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttg                                                                                                                                      <  1:964323/150‑2 (MQ=255)

                 cATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttgc                                                                                                                                     <  1:1443296/150‑3 (MQ=255)

                 cATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTc                                                                                                                                     <  1:490519/150‑1 (MQ=255)

                  aTCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCt                                                                                                                                    <  1:1046271/150‑1 (MQ=255)

                    cAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCttt                                                                                                                                  <  2:979157/150‑1 (MQ=255)

                    cAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCttt                                                                                                                                  >  1:1505853/1‑150 (MQ=255)

                    cAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCttt                                                                                                                                  <  1:1376424/150‑1 (MQ=255)

                     acatatAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTg                                                                                                                                 <  2:886789/148‑1 (MQ=255)

                     aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGGTGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttgccaat                                                                                                                                 <  1:621580/150‑7 (MQ=255)

                     aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttgccaat                                                                                                                                 <  1:995751/150‑7 (MQ=255)

                     aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTg                                                                                                                                 <  2:1198934/150‑1 (MQ=255)

                       ttataTCGTATTGATTATATCCATTTTAAACTATGCGCTTAGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGAc                                                                                                                               <  2:399363/149‑1 (MQ=255)

                         ataGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGActa                                                                                                                             >  2:363798/1‑149 (MQ=255)

                          taGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGActct                                                                                                                            >  2:141126/1‑150 (MQ=255)

                          taGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGActct                                                                                                                            <  2:1623834/150‑1 (MQ=255)

                            gCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTAt                                                                                                                          >  2:616698/1‑150 (MQ=255)

                             cGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttgcca                                                                                                                                   <  1:967147/140‑5 (MQ=255)

                             cGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttgcca                                                                                                                                   >  2:967147/1‑136 (MQ=255)

                             cGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATg                                                                                                                         <  1:159896/150‑1 (MQ=255)

                              gTATTGATGATAGCCATTTTAAACTAATCGGTTCGTTTTGCAGGTTGATGTTTGTTATCATCACTGAACGAAAATAAAGCAGTAACCCGCAATTTGTGCGAATTATTGGCAAAAGGCAACCACACGCTGCCTTTTTCTTTGACTCTATGa                                                                                                                        <  2:574846/150‑1 (MQ=255)

                               tATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGAc                                                                                                                       >  2:20495/1‑150 (MQ=255)

                                 ttGATGATAGCCATTTTAAAGTTTGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACTAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGt                                                                                                                     <  1:295457/150‑1 (MQ=255)

                                  tgatgaTAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCTCAATGTTTGCGAATTATTGTCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGtt                                                                                                                    <  1:1545063/150‑1 (MQ=255)

                                   tatgatAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTa                                                                                                                   <  2:467734/149‑1 (MQ=255)

                                   gatgatAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTa                                                                                                                   >  1:1090506/1‑150 (MQ=255)

                                     tgatAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACa                                                                                                                 >  1:1586899/1‑150 (MQ=255)

                                     tgatAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACa                                                                                                                 >  2:197429/1‑150 (MQ=255)

                                        tAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAAACACAGGCTGCATTTTTCTTTGACTCTATGAAGTTAAAAAg                                                                                                              >  1:693332/1‑150 (MQ=255)

                                           ccATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTa                                                                                                           <  1:20495/150‑1 (MQ=255)

                                             aTTTTAAACTATTCGCTTCGTTTTGCAGGTTGATGTTTGTTATCATCACTCAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCATAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAat                                                                                                         <  2:861535/150‑1 (MQ=255)

                                             aTTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAat                                                                                                         <  2:603938/150‑1 (MQ=255)

                                              ttttAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGGTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAata                                                                                                        <  2:1289132/150‑1 (MQ=255)

                                              ttttAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAata                                                                                                        <  2:1664403/150‑1 (MQ=255)

                                              ttttAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAata                                                                                                        <  1:724692/150‑1 (MQ=255)

                                              ttttAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAata                                                                                                        <  2:1161149/150‑1 (MQ=255)

                                               tttAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAatat                                                                                                       <  2:924452/150‑1 (MQ=255)

                                                 tAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAg                                                                                                                                                 >  2:1444050/1‑106 (MQ=255)

                                                         gcgcTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGGAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTa                                                                                             <  1:1488166/150‑1 (MQ=255)

                                                         gcgcTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTa                                                                                             <  1:835869/150‑1 (MQ=255)

                                                            cTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAGGTTAATATGCGCGCCCTATGc                                                                                          <  1:1438429/150‑1 (MQ=255)

                                                               cGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCaaa                                                                                       <  1:1500534/150‑1 (MQ=255)

                                                                gTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCaaaa                                                                                      <  2:460936/150‑1 (MQ=255)

                                                                  tttGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAgg                                                                                    >  2:894597/1‑150 (MQ=255)

                                                                      cAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTaaa                                                                                >  1:589369/1‑150 (MQ=255)

                                                                        ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                              >  2:248928/1‑150 (MQ=255)

                                                                        ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                              <  2:383212/150‑1 (MQ=255)

                                                                           tGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTAc                                                                           >  2:884108/1‑150 (MQ=255)

                                                                              tgtttgttATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAAACACAGGCTGCCTTTTTATTTGAATCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTAcccc                                                                        >  2:1178622/1‑150 (MQ=255)

                                                                               gtttgttATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCa                                                                                         <  2:1157085/132‑1 (MQ=255)

                                                                                  tgttATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGAc                                                                    <  1:80196/150‑1 (MQ=255)

                                                                                   gttATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGAct                                                                   >  2:328279/1‑150 (MQ=255)

                                                                                     tATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGa                                                                                                                        <  1:1515528/95‑1 (MQ=255)

                                                                                     tATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGa                                                                                                                        >  2:1515528/1‑95 (MQ=255)

                                                                                      aTCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGCCGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACtctc                                                                <  1:1671879/150‑1 (MQ=255)

                                                                                        cAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGa                                                              >  2:1099116/1‑150 (MQ=255)

                                                                                          gCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATc                                                            >  1:1082968/1‑150 (MQ=255)

                                                                                           cACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAAACACAGGCAGCCTTTTTCTTTGACTCTAAGACGTTAAAAAGTTAATATGCGCGCCCTATGCAAAATGTAAAATTACACCTGACTCTCGATcc                                                           >  2:1422584/1‑150 (MQ=255)

                                                                                             cTGAACGAAAATAAAGAAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACCCTATGACGTTACAAAGTTAATATGCGCCCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCgg                                                         >  1:689503/1‑150 (MQ=255)

                                                                                              tGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGt                                                        <  2:931685/150‑1 (MQ=255)

                                                                                               gAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCCTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGtt                                                       >  2:1086860/1‑150 (MQ=255)

                                                                                                 aCGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCg                                                     >  1:545680/1‑150 (MQ=255)

                                                                                                   gAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTa                                                   >  2:430099/1‑150 (MQ=255)

                                                                                                      aaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGt                                                    >  1:1485438/1‑146 (MQ=255)

                                                                                                      aaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGt                                                    <  2:1485438/146‑1 (MQ=255)

                                                                                                       aTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCTCAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGc                                               <  2:449053/150‑1 (MQ=255)

                                                                                                         aaaGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCCTACGGCTc                                             >  2:57128/1‑150 (MQ=255)

                                                                                                            gCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCaaa                                          <  2:721524/150‑1 (MQ=255)

                                                                                                                tAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACg                                      <  2:1082968/150‑1 (MQ=255)

                                                                                                                 aaCCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTAACTCTCGATCCGGTTCGTACGGCTCAAAAACGc                                     >  2:1271812/1‑150 (MQ=255)

                                                                                                                  aCCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGcc                                    <  1:904209/150‑1 (MQ=255)

                                                                                                                     cGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTg                                 <  1:616698/150‑1 (MQ=255)

                                                                                                                     cGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTg                                 <  2:481473/150‑1 (MQ=255)

                                                                                                                           gtgtgCGAAATATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCTATCCGGTGCGTACGGCTCAAAAACGCCTTGATTAcc                           <  2:217292/150‑1 (MQ=255)

                                                                                                                               gCGAATTATTTGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAggg                       <  1:654289/150‑1 (MQ=255)

                                                                                                                               gCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAggg                       >  2:169457/1‑150 (MQ=255)

                                                                                                                               gCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAggg                       >  2:723970/1‑150 (MQ=255)

                                                                                                                                 gAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTa                     >  1:1253905/1‑150 (MQ=255)

                                                                                                                                      attGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCtat                <  2:589369/150‑1 (MQ=255)

                                                                                                                                        aagaaaaaGGCAGCCTGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAc              >  1:1201294/5‑150 (MQ=255)

                                                                                                                                        aagaaaaaGGCAGCCTGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAc              >  1:1195444/5‑150 (MQ=255)

                                                                                                                                         ggCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcc             >  1:1089008/1‑150 (MQ=255)

                                                                                                                                         ggCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcc             <  2:1586899/150‑1 (MQ=255)

                                                                                                                                         ggCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcc             >  2:1570496/1‑150 (MQ=255)

                                                                                                                                         ggCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcc             >  2:1343200/1‑150 (MQ=255)

                                                                                                                                           cAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcccc           >  1:164214/1‑150 (MQ=255)

                                                                                                                                           cAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcccc           >  2:528615/1‑150 (MQ=255)

                                                                                                                                           aaaaaGGCAGCCTGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcccc           >  1:1008500/2‑150 (MQ=255)

                                                                                                                                                ggCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATc      >  2:1524215/1‑150 (MQ=255)

                                                                                                                                                 gCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCa     >  1:261613/1‑150 (MQ=255)

                                                                                                                                                   aaCCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAgg   <  1:353802/150‑1 (MQ=255)

                                                                                                                                                    aCCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGt  >  1:1514391/1‑150 (MQ=255)

                                                                                                                                                    aCCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGt  <  1:430099/150‑1 (MQ=255)

TTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGT  >  NC_000913/1146588‑1146885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`