Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,932,094 C→G 6.4% intergenic (+35/+21) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,932,0940CG 6.4% 318.5 / 9.9 94intergenic (+35/+21)purT/edaphosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase
Reads supporting (aligned to +/- strand):  ref base C (38/50);  new base G (4/2);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

GCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGG  >  NC_000913/1931945‑1932241
                                                                                                                                                     |                                                                                                                                                   
gCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATcc                                                                                                                                                     >  2:324397/1‑150 (MQ=255)
gCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATcc                                                                                                                                                     >  1:3011/1‑150 (MQ=255)
gCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATcc                                                                                                                                                     >  1:9204/1‑150 (MQ=255)
gCCGGAAATTGATGGCAGCCGTCGTATGGGGGTGGAACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAAACCGGCAAAAAAACGCTACAAAAATGCCCGATcc                                                                                                                                                     >  2:139001/1‑150 (MQ=255)
  cGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTc                                                                                                                                                   >  1:144755/1‑150 (MQ=255)
           aTGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCa                                                                                                                                          <  1:791501/150‑1 (MQ=255)
            tGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAt                                                                                                                                         <  2:1049170/150‑1 (MQ=255)
              gCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt                                                                                                                                       >  2:1373882/1‑150 (MQ=255)
               cAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                                                                                                      >  2:273794/1‑150 (MQ=255)
               cAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                                                                                                      <  2:179844/150‑1 (MQ=255)
               cAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                                                                                                      >  1:664561/1‑150 (MQ=255)
                aGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTg                                                                                                                                     <  1:1304254/150‑1 (MQ=255)
                aGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTg                                                                                                                                     <  2:510782/150‑1 (MQ=255)
                 gCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttttg                                                                                                                                    <  2:85294/150‑3 (MQ=255)
                 gCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa                                                                                                                                    <  1:929709/150‑1 (MQ=255)
                   cgtcgtCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACt                                                                                                                                  <  1:1438500/150‑1 (MQ=255)
                   cgtcgtCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACt                                                                                                                                  <  2:177196/150‑1 (MQ=255)
                    gtcgtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                                                                                                                 >  2:83015/1‑150 (MQ=255)
                      cgtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt                                                                                                                               <  2:86502/150‑1 (MQ=255)
                       gtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                                                                                                              >  2:1632958/1‑150 (MQ=255)
                          tGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAg                                                                                                                           <  1:329209/150‑1 (MQ=255)
                             gggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGTACATGTAAAAGTACATGGTTAAACCCGGCAAAAAATCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCtt                                                                                                                        <  2:1219849/150‑1 (MQ=255)
                              ggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                                                                                                       <  1:1423326/150‑1 (MQ=255)
                                tGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                                                                                                                     <  2:144755/150‑1 (MQ=255)
                                 ggCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcg                                                                                                                    <  2:275663/150‑1 (MQ=255)
                                 ggCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcg                                                                                                                    <  1:89158/150‑1 (MQ=255)
                                      tGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTc                                                                                                               <  2:636156/150‑1 (MQ=255)
                                          tACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACa                                                                                                           <  2:664561/150‑1 (MQ=255)
                                            cTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGc                                                                                                         <  1:178740/150‑1 (MQ=255)
                                              gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAACCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                                                                                                       <  2:1554236/150‑1 (MQ=255)
                                                agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCa                                                                                                     >  1:1545253/1‑150 (MQ=255)
                                                 gatagTGTTGTTGACGCCATTGAACTCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGGTAAAAAAATGCCCGATCCTCGATCGTGCATTTTGACTTTTACAGATTAGCGCCTTCTACAGCTTCAc                                                                                                    <  2:1393212/147‑1 (MQ=255)
                                                   gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc                                                                                                  >  1:58619/1‑150 (MQ=255)
                                                   gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc                                                                                                  <  1:1141162/150‑1 (MQ=255)
                                                          gttgACGCCATTGAACGCGCGAAGCACGCCGGCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt                                                                                           <  2:1102249/150‑1 (MQ=255)
                                                          gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt                                                                                           >  1:636310/1‑150 (MQ=255)
                                                           ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCtt                                                                                          <  2:47817/150‑1 (MQ=255)
                                                           ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCtt                                                                                          <  2:233673/150‑1 (MQ=255)
                                                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg                                                                                        >  1:692174/1‑150 (MQ=255)
                                                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg                                                                                        <  1:693265/150‑1 (MQ=255)
                                                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg                                                                                        <  1:1087329/150‑1 (MQ=255)
                                                                gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTaa                                                                                     >  2:1188190/1‑150 (MQ=255)
                                                                      gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGt                                                                               >  1:1513226/1‑150 (MQ=255)
                                                                        aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                             <  1:1593607/150‑1 (MQ=255)
                                                                        aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                             <  2:1084912/150‑1 (MQ=255)
                                                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                            <  1:1661445/150‑1 (MQ=255)
                                                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                            >  2:792041/1‑150 (MQ=255)
                                                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                            >  2:330416/1‑150 (MQ=255)
                                                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                            >  1:857055/1‑150 (MQ=255)
                                                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                            >  1:797004/1‑150 (MQ=255)
                                                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                            <  1:324397/150‑1 (MQ=255)
                                                                           cgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACCGCTTCAGGCGCCAGCTTAGTAATGCGGTCGTaa                                                                          >  2:1253194/1‑150 (MQ=255)
                                                                            gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAAt                                                                         >  1:1504287/1‑150 (MQ=255)
                                                                            gcgAAGCACGCCGCAGGACAGGTAAAACGACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAAt                                                                         <  2:1282125/150‑1 (MQ=255)
                                                                                 gctcgccgcCTGACAGGTAAAAGTACATGGTTAAACCCGGCAAATAAACGCTAAAAAAATGCGCGATCCTCGATCGGGTATTTTGACTTTTACAGCTTAGCGCGTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCTTAATctcac                                                                    <  2:717313/147‑5 (MQ=255)
                                                                                 gCACGCCGCCGTACAGTTAAAACTACAAGGTAAAACAAGACAAAAAAAAACTAAAAAAATGACCTATCCTCGATCTGTCATTTTTAGATTTAAAGATTAAAGAATTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGccc                                                                    >  2:683920/1‑150 (MQ=255)
                                                                                 gCACGCCGCCGGACAGGTAAAAGTAGAGTGTTAAACCCTGCAAAATAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGccc                                                                    <  2:1655772/150‑1 (MQ=255)
                                                                                  cACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGt                                                                               <  2:1142257/138‑1 (MQ=255)
                                                                                  cACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGt                                                                               >  1:1142257/1‑138 (MQ=255)
                                                                                    cgccgcCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCt                                                                 >  2:1320683/1‑150 (MQ=255)
                                                                                         ccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAg                                                            >  2:1504207/1‑150 (MQ=255)
                                                                                           ggACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgcg                                                          >  2:499031/1‑150 (MQ=255)
                                                                                            gACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgcgc                                                         <  2:1545253/150‑1 (MQ=255)
                                                                                              cAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCAt                                                       >  1:1126513/1‑150 (MQ=255)
                                                                                                ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATcc                                                     >  1:329954/1‑149 (MQ=255)
                                                                                                ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCt                                                     >  1:532730/1‑150 (MQ=255)
                                                                                                ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCt                                                     >  2:33329/1‑150 (MQ=255)
                                                                                                ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCt                                                     >  2:1127108/1‑150 (MQ=255)
                                                                                                 gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                    <  1:1632958/150‑1 (MQ=255)
                                                                                                    aaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCg                                                 >  2:728219/1‑150 (MQ=255)
                                                                                                    aaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGATTTTTATAGTTTCACGCGCAAGCTTATTATTTCGGTCGTAATAGCCCGTTTCCATCGAATATGCCg                                                 <  2:920006/150‑1 (MQ=255)
                                                                                                     aaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCgg                                                >  2:349215/1‑150 (MQ=255)
                                                                                                         aCAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAcc                                            <  1:975565/150‑1 (MQ=255)
                                                                                                           aGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccag                                          <  1:216900/150‑1 (MQ=255)
                                                                                                           aGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccag                                          <  1:514911/150‑1 (MQ=255)
                                                                                                            gggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagc                                         <  2:857055/150‑1 (MQ=255)
                                                                                                                   aCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAac                                  <  1:842848/150‑1 (MQ=255)
                                                                                                                      cGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAaccac                               >  2:181092/1‑150 (MQ=255)
                                                                                                                           aaaaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATg                          <  2:1047900/150‑1 (MQ=255)
                                                                                                                              aaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGcac                       <  1:349215/150‑1 (MQ=255)
                                                                                                                              aaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGcac                       <  2:58619/150‑1 (MQ=255)
                                                                                                                               aaCGCTACAAAAATGCCCGGTCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCTTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGcaca                      >  2:1570968/1‑150 (MQ=255)
                                                                                                                                  gCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCa                   <  2:1504287/150‑1 (MQ=255)
                                                                                                                                   cTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCAc                  <  1:139001/150‑1 (MQ=255)
                                                                                                                                    tACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACg                 >  1:1024214/1‑150 (MQ=255)
                                                                                                                                      cAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCt               >  1:164928/1‑150 (MQ=255)
                                                                                                                                       aaaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcc                                        <  1:1249133/124‑1 (MQ=255)
                                                                                                                                       aaaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcc                                        >  2:1249133/1‑124 (MQ=255)
                                                                                                                                        aaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCttt             <  1:116157/150‑1 (MQ=255)
                                                                                                                                        aaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCttc             >  2:187821/1‑149 (MQ=255)
                                                                                                                                         aaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGACCCACCGATGCACAGCACGCtttt            >  2:679268/1‑150 (MQ=255)
                                                                                                                                          aaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTc           >  1:1086005/1‑150 (MQ=255)
                                                                                                                                           aTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCa          >  2:566331/1‑150 (MQ=255)
                                                                                                                                           aTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCa          >  1:409346/1‑150 (MQ=255)
                                                                                                                                              cccGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAgcg       <  1:728219/150‑1 (MQ=255)
                                                                                                                                                cGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGcc     >  2:1139204/1‑150 (MQ=255)
                                                                                                                                                cGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGcc     >  2:1144845/1‑150 (MQ=255)
                                                                                                                                                   tCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAgg  <  1:1373882/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGG  >  NC_000913/1931945‑1932241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑