Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,477,018	T→A	9.3%	R204S (AGA→AGT) ‡	dsdC ←	DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,018	0	T	A	9.3%	158.9 / 6.4	63	R204S (AGA→AGT) ‡	dsdC	DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base T (19/37);  new base A (4/2);  total (23/40)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01

CCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAA  >  NC_000913/2476869‑2477163                                                                                                                                                      |                                                                                                                                                    cccATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctgt                                                                                                                                                     <  1:1424032/150‑1 (MQ=255)  ccATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctgtc                                                                                                                                                    <  2:555003/150‑1 (MQ=255)  ccATCGCCACCCAAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGCAGTCGTTGAACCATGAcagtc                                                                                                                                                    >  2:995573/1‑150 (MQ=255)   cATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGAATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctgtct                                                                                                                                                   >  2:1381760/1‑150 (MQ=255)       gCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCa                                                                                                                                               >  1:966027/1‑150 (MQ=255)        ccACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAt                                                                                                                                              <  1:116612/150‑1 (MQ=255)          aCCACAATATGATTCATCGCGGGAATAACAGGTAAATCAGAAGGATCATAGCCAATTCCAGAAGATGTCTGCAAATTAACCTCATATTGTTGCGCCCAACTATGGCATTCATGCGTTCCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑gt                                                                                                                                           <  2:977610/150‑2 (MQ=255)           ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGa                                                                                                                                          <  2:788330/150‑1 (MQ=255)               aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGGCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCAT‑GGAGCaa                                                                                                                                      <  1:661667/150‑1 (MQ=255)                 tatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCAT‑GGAGCAACg                                                                                                                                    <  2:386974/150‑1 (MQ=255)                   tGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTa                                                                                                                                  <  1:640753/150‑1 (MQ=255)                   tGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTa                                                                                                                                  <  2:964585/150‑1 (MQ=255)                     aTTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑TGAGCAACGTACa                                                                                                                                >  2:1112694/1‑150 (MQ=255)                       tCATCGCGGCAATAACAGCTAAATCAGAACGATCAAATCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGt                                                                                                                              <  2:1284113/150‑1 (MQ=255)                       tCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGGTACAATCCGGTATGTGAA‑GGAGCAACCTaaact                                                                                                                              >  2:1487709/1‑146 (MQ=255)                       tCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGt                                                                                                                              <  1:286642/150‑1 (MQ=255)                        cATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTg                                                                                                                             <  2:624189/150‑1 (MQ=255)                        cATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTg                                                                                                                             <  1:1446786/150‑1 (MQ=255)                          tCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGac                                                                                                                           <  1:1000198/150‑1 (MQ=255)                           cgcgGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCTCCATGGCGGTGTGTGAT‑GGGGCAACGTACAGTGgca                                                                                                                          >  1:981407/1‑150 (MQ=255)                            gcgGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGacac                                                                                                                         >  2:36548/1‑150 (MQ=255)                            gcgGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCGCCAAGCCTGTCTGTCAT‑GGAGCAACGTACAGTGccac                                                                                                                         >  2:1047620/1‑150 (MQ=255)                                    aaCAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAAt                                                                                                                <  1:1037839/150‑1 (MQ=255)                                     aCAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAAtt                                                                                                               <  2:836455/150‑1 (MQ=255)                                      cAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTa                                                                                                              <  1:1665445/150‑1 (MQ=255)                                           aaaTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCgtg                                                                                                         >  2:683892/1‑150 (MQ=255)                                             aTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTGTACAATCCTGTGTGTGATGGGGGCAAC‑TAAAGTGACACAGGGTTAATTaactggt                                                                                                        >  1:1271853/1‑144 (MQ=255)                                                   aCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTaa                                                                                                 >  1:1195276/1‑150 (MQ=255)                                                       tCAAAGCCAATTCCCGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGGCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCa                                                                                             <  1:1439917/150‑1 (MQ=255)                                                           aGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTc                                                                                         <  1:186888/150‑1 (MQ=255)                                                             ccAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCtt                                                                                       <  1:683892/150‑1 (MQ=255)                                                               aaTTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCTACATGCCGGTCTGTAAT‑GGGGCAACCTACAGTGACACA‑CGTTAATTACCGGGGTGGTTAAAGCCTGTCGTTg                                                                                     >  1:283177/1‑150 (MQ=255)                                                                    cAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCAAGCCAGTGTGTGAA‑GGAGCAACGTTCAGTGACACA‑GGTTAATTAACGGGTTGGGTAAAGCATGTGTTTGAGCgg                                                                                >  2:470380/1‑149 (MQ=255)                                                                        agaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCTCCAAGCCGGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTTATTAACGTGTTGGTTAAAGCATGTGTTTGAGCGTAtta                                                                            >  2:1336622/1‑149 (MQ=255)                                                                         gaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTcc                                                                           <  2:1276434/150‑1 (MQ=255)                                                                             tCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                       <  2:948645/150‑1 (MQ=255)                                                                                gCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACGGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTAc                                                                    <  2:471049/150‑1 (MQ=255)                                                                                        aCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGc                                                            <  1:1422443/150‑1 (MQ=255)                                                                                            cATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GCTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGa                                                        <  2:786867/150‑1 (MQ=255)                                                                                            aaTAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGa                                                        <  1:1381760/149‑1 (MQ=255)                                                                                                  gTTGCGCCGAACTATGCCATTCATCCGTAGCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttctt                                                  <  1:1187853/150‑1 (MQ=255)                                                                                                        cccAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatcca                                            <  2:961805/150‑1 (MQ=255)                                                                                                                 gCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGt                                   <  1:1487709/150‑1 (MQ=255)                                                                                                                  ccATTCATCCGTACCGGAGTCGTTTCTACAATCCAGTATGTTAT‑GGAGCAACGTACAATTAAAAA‑CGTTAATTACCGTGTTGGTTTAAGAATGTATTTGAGCGTATTACGGGCGACAAAAAGGCAGGATTTATTAATACAAAAGaaaatt                                  >  2:654235/1‑147 (MQ=255)                                                                                                                    aTTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgat                                <  1:1047058/150‑1 (MQ=255)                                                                                                                      tCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatga                              <  1:36548/150‑1 (MQ=255)                                                                                                                           cGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgagtgag                         <  1:1336622/150‑1 (MQ=255)                                                                                                                               cccgactCGTTGCTCCATGCCTGTGTGTCAT‑GGAGCAAAGTAGAGTTACACA‑GGTTAATTACCGTGTTTGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATAATGGCAGGAGTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgg                     <  2:1245168/144‑2 (MQ=255)                                                                                                                               ccGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgc                     <  1:508988/150‑1 (MQ=255)                                                                                                                                 ggAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgcgc                   <  2:1195276/150‑1 (MQ=255)                                                                                                                                 ggAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgcgc                   <  1:61537/150‑1 (MQ=255)                                                                                                                                    gTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGa                >  1:821031/1‑150 (MQ=255)                                                                                                                                        ttGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACggn            <  1:654235/150‑2 (MQ=255)                                                                                                                                        ttGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgc            <  1:1217332/150‑1 (MQ=255)                                                                                                                                         tGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcg           <  1:1288828/150‑1 (MQ=255)                                                                                                                                          gCTCCATGACAGACAGGCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc          >  2:673262/1‑150 (MQ=255)                                                                                                                                          gCTCCATGACAGACAGGCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc          >  1:860627/1‑150 (MQ=255)                                                                                                                                          gCTCCATGACAGACAGGCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc          >  2:1552216/1‑150 (MQ=255)                                                                                                                                          gCTCCATGACAGACAGGCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc          >  1:1353226/1‑150 (MQ=255)                                                                                                                                           cTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca         >  2:484622/1‑150 (MQ=255)                                                                                                                                             ccATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatc       >  2:225453/1‑150 (MQ=255)                                                                                                                                             ccATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatc       >  1:1014146/1‑150 (MQ=255)                                                                                                                                               aTGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactc     <  1:995573/150‑5 (MQ=255)                                                                                                                                                tGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCatcatc    >  2:403244/1‑150 (MQ=255)                                                                                                                                                tGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCatcatc    <  1:219472/150‑1 (MQ=255)                                                                                                                                                tGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCatcatc    >  1:1413854/1‑150 (MQ=255)                                                                                                                                                 gCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCAtcatca   <  1:225453/150‑1 (MQ=255)                                                                                                                                                  ccgttctgtcTT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCTTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCaa  <  1:580892/146‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAT‑GGAGCAACGTACAGTGACACA‑GGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAA  >  NC_000913/2476869‑2477163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑