BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,491,266	C→G	5.1%	E229Q (GAA→CAA)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,491,266	0	C	G	5.1%	443.1 / 9.4	129	E229Q (GAA→CAA)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand): ref base C (71/50); new base G (4/3); total (75/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

CCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACGCTT  >  NC_000913/2491118‑2491415

ccAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtca                                                                                                                                                       >  1:238281/1‑150 (MQ=255)

ccAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtca                                                                                                                                                       <  1:1394652/150‑1 (MQ=255)

 caacaaGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGCAGCTGTtcat                                                                                                                                                      <  1:1019953/150‑1 (MQ=255)

  aacaaGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatc                                                                                                                                                     >  2:1426676/1‑150 (MQ=255)

   acaaGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatca                                                                                                                                                    <  2:467198/150‑1 (MQ=255)

      aGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCt                                                                                                                                                 >  2:1461593/1‑150 (MQ=255)

          tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑gtg                                                                                                                                            <  2:1042511/150‑1 (MQ=255)

          tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑gtg                                                                                                                                            >  1:1198640/1‑150 (MQ=255)

          tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAACTGGATGCGTATCTTCTATGTTCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCTCGAAGCTGTTGATCAGCTt‑gtg                                                                                                                                            <  2:1603975/150‑1 (MQ=255)

           gACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGATGAACAGCttcgtg                                                                                                                                            <  1:325885/150‑5 (MQ=255)

            aCAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGaa                                                                                                                                          >  2:334014/1‑150 (MQ=255)

            aCAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCAATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGaa                                                                                                                                          >  1:892340/1‑150 (MQ=255)

                cGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcg                                                                                                                                      >  2:619648/1‑150 (MQ=255)

                  tCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgcc                                                                                                                                    >  1:1670460/1‑150 (MQ=255)

                   cAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgccg                                                                                                                                   >  1:1133326/1‑150 (MQ=255)

                    ttcgtttGCCCTGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgccgc                                                                                                                                  >  1:1279696/5‑150 (MQ=255)

                     gCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCcgcg                                                                                                                                 >  1:209721/1‑150 (MQ=255)

                         ttGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCtt                                                                                                                             >  2:496215/1‑150 (MQ=255)

                          tGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCttt                                                                                                                            >  1:1288357/1‑150 (MQ=255)

                           gCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTg                                                                                                                           >  1:639580/1‑150 (MQ=255)

                           gCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTg                                                                                                                           <  1:463531/150‑1 (MQ=255)

                            ccAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAGGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGc                                                                                                                          <  1:495662/150‑1 (MQ=255)

                               gggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGATGAACAGCttcgtg                                                                                                                                            >  2:1205565/1‑126 (MQ=255)

                               gggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGATGAACAGCttcgtg                                                                                                                                            <  1:1205565/130‑5 (MQ=255)

                                ggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAg                                                                                                                      >  1:1057782/1‑150 (MQ=255)

                                ggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAg                                                                                                                      <  1:291246/150‑1 (MQ=255)

                                   aaaCGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAt                                                                                                                   >  1:554938/1‑150 (MQ=255)

                                    aacgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAta                                                                                                                  >  2:1407914/1‑150 (MQ=255)

                                    aacgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTAAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAta                                                                                                                  >  2:470006/1‑150 (MQ=255)

                                      cgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGTAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaat                                                                                                                >  1:56287/1‑150 (MQ=255)

                                      cgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaat                                                                                                                >  2:348577/1‑150 (MQ=255)

                                       gaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaatt                                                                                                               <  2:209721/150‑1 (MQ=255)

                                             gcAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGc                                                                                                         >  1:1052470/1‑150 (MQ=255)

                                             gcAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGc                                                                                                         <  1:619648/150‑1 (MQ=255)

                                                gCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGt                                                                                                      >  1:1411610/1‑150 (MQ=255)

                                                  tGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTc                                                                                                    <  2:344272/150‑1 (MQ=255)

                                                  tGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTc                                                                                                    <  2:1028933/150‑1 (MQ=255)

                                                  tGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTc                                                                                                    <  2:238281/150‑1 (MQ=255)

                                                    ccGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAg                                                                                                  >  2:240508/1‑150 (MQ=255)

                                                      gCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCt                                                                                                <  1:496215/150‑1 (MQ=255)

                                                      gCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCt                                                                                                >  2:267011/1‑150 (MQ=255)

                                                         gAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCtttt                                                                                             >  2:53198/1‑150 (MQ=255)

                                                           aaGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGc                                                                                           <  2:500103/150‑1 (MQ=255)

                                                           aaGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGc                                                                                           <  2:254436/150‑1 (MQ=255)

                                                               ggATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCt                                                                                          <  2:1175756/147‑1 (MQ=255)

                                                               ggATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTaaa                                                                                       >  2:1115495/1‑150 (MQ=255)

                                                                gATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCACGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTaaaa                                                                                      <  1:1009/150‑1 (MQ=255)

                                                                 aTGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAg                                                                                     <  1:334014/150‑1 (MQ=255)

                                                                     gTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGaa                                                                                 >  2:620724/1‑150 (MQ=255)

                                                                         cTTCAAGGATCCCTTTCGCCATAGACATTGGCAGTAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTg                                                                             <  2:56287/150‑1 (MQ=255)

                                                                         cTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTg                                                                             >  1:31085/1‑150 (MQ=255)

                                                                         cTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTg                                                                             >  2:1302613/1‑150 (MQ=255)

                                                                          ttCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgc                                                                            >  2:677238/1‑150 (MQ=255)

                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAACTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgcgc                                                                          <  1:1209742/150‑1 (MQ=255)

                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgcgc                                                                          >  2:175604/1‑150 (MQ=255)

                                                                                   cccTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgac                                                                   >  2:1157918/1‑150 (MQ=255)

                                                                                    ccTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgact                                                                  >  2:1141047/1‑150 (MQ=255)

                                                                                    ccTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGAGCTAGtgact                                                                  >  2:400439/1‑150 (MQ=255)

                                                                                    ccTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTATAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGagact                                                                  >  1:1116709/1‑150 (MQ=255)

                                                                                           ccATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCg                                                           >  1:1361020/1‑150 (MQ=255)

                                                                                           ccATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCg                                                           <  1:1426676/150‑1 (MQ=255)

                                                                                            cATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCgg                                                          <  2:1361020/150‑1 (MQ=255)

                                                                                             aTAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCggg                                                         >  2:1399099/1‑150 (MQ=255)

                                                                                             aTAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCggg                                                         <  1:677238/150‑1 (MQ=255)

                                                                                                   aTTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGc                                                   <  2:31085/150‑1 (MQ=255)

                                                                                                         aGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCaataat                                             <  2:1302266/150‑1 (MQ=255)

                                                                                                           gaatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGc                                           >  2:960879/1‑150 (MQ=255)

                                                                                                            aatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCt                                          >  2:1334926/1‑150 (MQ=255)

                                                                                                                 aatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCgc                                     >  2:1221844/1‑149 (MQ=255)

                                                                                                                    cTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGAccg                                  >  1:266005/1‑148 (MQ=255)

                                                                                                                     tGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCa                                                       >  1:605417/1‑128 (MQ=255)

                                                                                                                     tGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGa                                 >  2:935393/1‑150 (MQ=255)

                                                                                                                      gAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAt                                <  1:367218/150‑1 (MQ=255)

                                                                                                                         cACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAtttt                             >  1:767100/1‑150 (MQ=255)

                                                                                                                          aCTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAATAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTc                            >  2:1370748/1‑150 (MQ=255)

                                                                                                                          aCTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTc                            <  2:554938/150‑1 (MQ=255)

                                                                                                                              tCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGACAAGGATAATTAATGGCCGTTCAGATTTTGCTAAAAGCGAAATTGCGCTACTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCCACt                        >  2:678528/1‑150 (MQ=255)

                                                                                                                               cAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACtt                       >  2:1585253/1‑150 (MQ=255)

                                                                                                                               cAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACtt                       >  2:78469/1‑150 (MQ=255)

                                                                                                                               cAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAAGAATGCTGGCGCCGGATTTTCAACtt                       >  2:1552810/1‑150 (MQ=255)

                                                                                                                                 aTAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCTGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTa                     <  1:1620418/150‑1 (MQ=255)

                                                                                                                                 aTAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACttga                     >  1:437706/1‑148 (MQ=255)

                                                                                                                                 aTAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTa                     <  1:1157918/150‑1 (MQ=255)

                                                                                                                                  cgtaTTCAC‑AAGCTGATGAACAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAAc                   >  2:169151/4‑150 (MQ=255)

                                                                                                                                   aaaTTCACGAAGCTTTTAATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGTATTTTCAACTTTatc                   <  2:881505/150‑3 (MQ=255)

                                                                                                                                      ttCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAAt                >  1:809056/1‑150 (MQ=255)

                                                                                                                                        cACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCg              <  2:172922/150‑1 (MQ=255)

                                                                                                                                        cACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCg              >  2:708102/1‑150 (MQ=255)

                                                                                                                                         cacaaGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCg              >  1:1041692/4‑150 (MQ=255)

                                                                                                                                         cacaaGCTGATGAACAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGt             >  1:353280/4‑150 (MQ=255)

                                                                                                                                           gAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACttt                      >  2:66737/1‑139 (MQ=255)

                                                                                                                                           gAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTgg           <  1:1495020/150‑1 (MQ=255)

                                                                                                                                             aGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGtt         <  1:509740/150‑1 (MQ=255)

                                                                                                                                              gCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTa        <  2:50872/150‑1 (MQ=255)

                                                                                                                                              gCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTa        <  1:446449/150‑1 (MQ=255)

                                                                                                                                                tGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAAc      <  1:1462583/150‑1 (MQ=255)

                                                                                                                                                 gTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACg     <  1:1369827/150‑1 (MQ=255)

                                                                                                                                                 gTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACg     >  2:75040/1‑150 (MQ=255)

                                                                                                                                                   tcatcaGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACGCt   <  2:786601/150‑1 (MQ=255)

                                                                                                                                                    catcaGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACGCtt  >  2:1246052/1‑150 (MQ=255)

CCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACGCTT  >  NC_000913/2491118‑2491415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`