Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,661,482	G→C	7.9%	P17R (CCG→CGG)	iscS ←	cysteine desulfurase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,482	0	G	C	7.9%	284.4 / 11.0	88	P17R (CCG→CGG)	iscS	cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base G (41/40);  new base C (2/5);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

GCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATT  >  NC_000913/2661333‑2661628                                                                                                                                                       |                                                                                                                                                   gcgGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CAcgcg                                                                                                                                                    >  1:171521/1‑150 (MQ=255)   ggATCAGCGCCGACCAGATCGTCAATCTGATTACGGGCTATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCggg                                                                                                                                                  <  1:1354223/150‑1 (MQ=255)    gATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGt                                                                                                                                                 >  1:195817/1‑150 (MQ=255)      agattgcCGATCTGGTCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTcc                                                                                                                                               >  1:989316/6‑150 (MQ=255)       cAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGGGAGAACGGGAGGCCGGGTCACCAAAGGGTCCGTCCATCGTCCTAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCa                                                                                                                                              <  2:677039/150‑1 (MQ=255)           gcCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCgg                                                                                                                                          >  1:423900/1‑150 (MQ=255)            ccGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGc                                                                                                                                         >  1:1590426/1‑150 (MQ=255)               aCCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTg                                                                                                                                      <  2:924215/150‑1 (MQ=255)                 cAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGt                                                                                                                                    <  1:1190127/150‑1 (MQ=255)                   gATCTGCAATCTGATTACGCGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCCGGTGGACCCGCGTgttgc                                                                                                                                  <  1:731627/150‑5 (MQ=255)                     tCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCCGGTGGACCCGCGTgttgccg                                                                                                                                <  1:104273/150‑7 (MQ=255)                     tCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGTgttgcc                                                                                                                                 >  2:204929/1‑145 (MQ=255)                       ggCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGCAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGa                                                                                                                              >  2:1552829/1‑150 (MQ=255)                       ggCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGa                                                                                                                              <  1:17871/150‑1 (MQ=255)                       ggCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGTgttgccga                                                                                                                               <  1:678368/150‑8 (MQ=38)                        gCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACTGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGag                                                                                                                             <  1:760896/150‑1 (MQ=255)                        gCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGag                                                                                                                             <  1:160087/150‑1 (MQ=255)                         cAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGagt                                                                                                                            <  1:773578/150‑1 (MQ=255)                           aTCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGT‑GTTGCCGagaaa                                                                                                                          <  1:204929/150‑4 (MQ=37)                            tCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGT‑GTTGCCGagaaaa                                                                                                                         <  1:730014/150‑5 (MQ=37)                             cTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTc                                                                                                                        <  1:271923/150‑1 (MQ=255)                              tGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCg                                                                                                                       >  2:1508031/1‑150 (MQ=255)                              tGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCg                                                                                                                       <  2:195817/150‑1 (MQ=255)                              tGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCg                                                                                                                       >  2:212149/1‑150 (MQ=255)                                  tACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGAt                                                                                                                   >  1:1033729/1‑150 (MQ=255)                                   aCGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATa                                                                                                                  >  1:306774/1‑150 (MQ=255)                                       gCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATc                                                                                                              <  1:1430331/150‑1 (MQ=255)                                         gATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCgg                                                                                                            <  2:1271872/150‑1 (MQ=255)                                           tatCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTa                                                                                                          >  1:1418135/1‑150 (MQ=255)                                            atCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACTGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTaa                                                                                                         <  1:1421252/150‑1 (MQ=255)                                             tCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAt                                                                                                        >  1:1225428/1‑150 (MQ=255)                                               tACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                                                                                                      >  1:226813/1‑150 (MQ=255)                                                aCCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTc                                                                                                     >  2:1194213/1‑150 (MQ=255)                                                aCCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTc                                                                                                     <  2:306774/150‑1 (MQ=255)                                                  cGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCAt                                                                                                   <  2:1444600/150‑1 (MQ=255)                                                   gCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCAtt                                                                                                  >  2:1006613/1‑150 (MQ=255)                                                     ttcttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGc                                                                                                <  2:500935/150‑1 (MQ=255)                                                       cttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc                                                                                              <  1:1006613/150‑1 (MQ=255)                                                       cttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc                                                                                              <  2:1418135/150‑1 (MQ=255)                                                       cttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctc                                                                                              <  1:298328/150‑1 (MQ=255)                                                        ttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctct                                                                                             <  2:1004501/150‑1 (MQ=255)                                                         tcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCta                                                                                            >  2:151244/1‑150 (MQ=255)                                                            gCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaa                                                                                         <  2:865838/150‑1 (MQ=255)                                                               tGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACt                                                                                      <  2:556966/150‑1 (MQ=255)                                                                gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaa                                                                                         <  1:1433426/146‑1 (MQ=255)                                                                gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATaa                                                                                         >  2:1433426/1‑146 (MQ=255)                                                                gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTc                                                                                     >  2:1482125/1‑150 (MQ=255)                                                                gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTc                                                                                     <  1:957305/150‑1 (MQ=255)                                                                  cAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTGATTTCATTGCTCTATAAACTCCg                                                                                   >  1:388041/1‑150 (MQ=255)                                                                  cAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCg                                                                                   >  2:968330/1‑150 (MQ=255)                                                                    gCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTa                                                                                 <  1:1601209/150‑1 (MQ=255)                                                                       gAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACAt                                                                              <  1:787839/150‑1 (MQ=255)                                                                       gAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACAt                                                                              >  2:787839/1‑150 (MQ=255)                                                                          aCGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCAc                                                                           >  1:1118553/1‑150 (MQ=255)                                                                             gTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCa                                                                        >  2:184367/1‑150 (MQ=255)                                                                               gagaACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAAt                                                                      >  1:1080792/1‑150 (MQ=255)                                                                                agaACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATg                                                                     >  2:74031/1‑150 (MQ=255)                                                                                  aaCGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCa                                                                   <  1:1552829/150‑1 (MQ=255)                                                                                    cgtgAGGCCGGGTTACCAAAGGTTCCTTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTTGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTATATAAACTCCGTACATCACTCAATGCAAg                                                                 <  1:1636164/147‑1 (MQ=255)                                                                                    cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAg                                                                 <  2:719462/150‑1 (MQ=255)                                                                                      ggAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGa                                                               >  2:1222276/1‑150 (MQ=255)                                                                                         ggCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATc                                                            <  2:1406872/150‑1 (MQ=255)                                                                                          gCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCa                                                           <  2:585845/150‑1 (MQ=255)                                                                                             gggTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGc                                                        <  1:1482125/150‑1 (MQ=255)                                                                                             gggTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGc                                                        <  2:423900/150‑1 (MQ=255)                                                                                                 tACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTAcc                                                    >  1:1141904/1‑150 (MQ=255)                                                                                                  aCCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCg                                                   >  1:1393283/1‑150 (MQ=255)                                                                                                   ccAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCgg                                                  >  2:448872/1‑150 (MQ=255)                                                                                                      aaGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTg                                               <  2:1225428/150‑1 (MQ=255)                                                                                                        ggTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGa                                             <  1:1508031/150‑1 (MQ=255)                                                                                                        ggTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGa                                             <  1:18169/150‑1 (MQ=255)                                                                                                          ttCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCTGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATg                                           <  1:1377931/150‑1 (MQ=255)                                                                                                             cGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTAc                                        >  1:845655/1‑150 (MQ=255)                                                                                                               tCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGa                                      >  2:1579761/1‑150 (MQ=255)                                                                                                                   tCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCgt                                  >  2:1559374/1‑150 (MQ=255)                                                                                                                      tCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGtt                               >  1:355870/1‑150 (MQ=255)                                                                                                                        aTAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTa                             <  2:989316/150‑1 (MQ=255)                                                                                                                          aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACg                           <  2:1219814/150‑1 (MQ=255)                                                                                                                               gCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTa                      >  2:597454/1‑150 (MQ=255)                                                                                                                                 atcatTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTAtt                    <  1:968330/150‑1 (MQ=255)                                                                                                                                 atcatTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTAtt                    >  1:1237504/1‑150 (MQ=255)                                                                                                                                    atTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAg                 <  1:312763/150‑1 (MQ=255)                                                                                                                                        tctcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACt             >  1:868292/1‑150 (MQ=255)                                                                                                                                         ctcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTc            >  2:617687/1‑150 (MQ=255)                                                                                                                                         ctcCGCAACCACGCCGGTGGACCCGCGT‑GTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTc            >  1:542129/1‑150 (MQ=255)                                                                                                                                          tcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTcc           >  2:339358/1‑150 (MQ=255)                                                                                                                                            ggCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCgg         >  1:1460027/1‑150 (MQ=255)                                                                                                                                             gCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGc        >  2:915660/1‑150 (MQ=255)                                                                                                                                             gCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTA‑TTAGCACTCCGGcc       >  1:1174881/1‑150 (MQ=255)                                                                                                                                              cAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGcc       >  2:137532/1‑150 (MQ=255)                                                                                                                                                    cgcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTACGGCCTGAtt  >  2:820581/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                   GCGGATCAGCGCCGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATT  >  NC_000913/2661333‑2661628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑