Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,701,536 A→T 5.9% L65Q (CTG→CAG)  pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,701,5360AT 5.9% 311.6 / 7.7 101L65Q (CTG→CAG) pdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (50/45);  new base T (4/2);  total (54/47)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 102 total aligned reads displayed.

CACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGC  >  NC_000913/2701390‑2701685
                                                                                                                                                  |                                                                                                                                                     
cACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCg                                                                                                                                                    >  1:1389067/1‑150 (MQ=255)
cACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCg                                                                                                                                                    <  1:458331/150‑1 (MQ=255)
  cGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTc                                                                                                                                                  >  1:1500907/1‑150 (MQ=255)
  cGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTc                                                                                                                                                  <  1:1218047/150‑1 (MQ=255)
    cTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTg                                                                                                                                                <  2:1142887/150‑1 (MQ=255)
     tGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTATTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGa                                                                                                                                               <  2:399568/150‑1 (MQ=255)
      gCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGAc                                                                                                                                              >  1:688964/1‑150 (MQ=255)
          tGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAg                                                                                                                                          >  1:281305/1‑150 (MQ=255)
           gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAgg                                                                                                                                         >  2:1653291/1‑150 (MQ=255)
            cGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCATAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTGAGCGCCATGTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGa                                                                                                                                        <  2:554036/150‑1 (MQ=255)
               cATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgc                                                                                                                                     >  2:357575/1‑150 (MQ=255)
                  ccAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCa                                                                                                                                  <  2:86648/150‑1 (MQ=255)
                  ccAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCa                                                                                                                                  <  1:1448142/150‑1 (MQ=255)
                   cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCTGCGTCAGACGCTGGAtacccgc                                                                                                                                 <  2:89208/150‑7 (MQ=255)
                   cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCTGCGTCAGACGCTGGAtacccgc                                                                                                                                 <  2:95275/150‑7 (MQ=255)
                   cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCAc                                                                                                                                 >  2:862603/1‑150 (MQ=255)
                      gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTc                                                                                                                              >  2:959755/1‑150 (MQ=255)
                      gccgccTTCGGTTGTTACTTACTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGACAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTc                                                                                                                              >  2:182276/1‑150 (MQ=255)
                       ccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcg                                                                                                                             <  2:673216/150‑1 (MQ=255)
                        cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                                                                            <  1:995193/150‑1 (MQ=255)
                        cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                                                                            <  1:357575/150‑1 (MQ=255)
                          ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                                                                          <  2:293715/150‑1 (MQ=255)
                                gttgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTa                                                                                                                    <  2:1156134/150‑1 (MQ=255)
                                  tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCa                                                                                                                                  >  1:440489/1‑134 (MQ=255)
                                  tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCa                                                                                                                                  <  2:440489/134‑1 (MQ=255)
                                   gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCTGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtg                                                                                                                 <  1:667437/150‑1 (MQ=255)
                                     tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtgtg                                                                                                               <  1:1283744/150‑1 (MQ=255)
                                          ccTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGc                                                                                                          <  1:240321/150‑1 (MQ=255)
                                            tGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGa                                                                                                        >  2:1094037/1‑150 (MQ=255)
                                             gACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGAt                                                                                                       >  2:380484/1‑150 (MQ=255)
                                              aCGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                                                                                                      <  1:871451/150‑1 (MQ=255)
                                              aCGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                                                                                                      <  1:990984/150‑1 (MQ=255)
                                              aCGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                                                                                                      <  1:886784/150‑1 (MQ=255)
                                                       cGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTAGGTCCCCGCCATCTCCAGATTCATGCGGGTATCCAGCCTCTTCCGCAGGATGCGCACGTCGCGGTAAGTAATGTGACGGCGATCTTCAAGTaa                                                                                             >  2:1052268/1‑150 (MQ=255)
                                                            ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa                                                                                        >  1:645294/1‑150 (MQ=255)
                                                            ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa                                                                                        >  2:106552/1‑150 (MQ=255)
                                                             cAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCAc                                                                                       >  1:78967/1‑150 (MQ=255)
                                                                    caAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATg                                                                                <  1:182276/150‑1 (MQ=255)
                                                                     aaaaaTGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGc                                                                               >  2:942844/1‑150 (MQ=255)
                                                                      aaaaTGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGcc                                                                              >  1:732337/1‑150 (MQ=255)
                                                                          tgtgGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTcc                                                                          <  1:1094037/150‑1 (MQ=255)
                                                                             ggCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt                                                                       >  2:565162/1‑150 (MQ=255)
                                                                               cTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTcc                                                                     >  2:337610/1‑150 (MQ=255)
                                                                                  cGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGc                                                                  >  2:830364/1‑150 (MQ=255)
                                                                                   gTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGcc                                                                 >  2:931328/1‑150 (MQ=255)
                                                                                     ctcAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg                                                               >  1:1580857/1‑150 (MQ=255)
                                                                                      tcAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg                                                               <  2:378884/149‑1 (MQ=255)
                                                                                      tcAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg                                                               >  1:378884/1‑149 (MQ=255)
                                                                                       cAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATTCCGTCCGCTCCCGCCTGCt                                                             >  1:1574697/1‑150 (MQ=255)
                                                                                       cAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCt                                                             >  2:1394784/1‑150 (MQ=255)
                                                                                       cAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCt                                                             >  2:774973/1‑150 (MQ=255)
                                                                                       cAACGGCGATCGCCAGCACCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCt                                                             <  1:446337/150‑1 (MQ=255)
                                                                                         aCGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCg                                                           <  2:1580857/150‑1 (MQ=255)
                                                                                         aCGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCg                                                           >  1:1604728/1‑150 (MQ=255)
                                                                                          cGGCGATCGACAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCgg                                                          >  2:1296499/1‑150 (MQ=255)
                                                                                           ggCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGc                                                         >  2:1655303/1‑150 (MQ=255)
                                                                                              gATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAt                                                      >  2:886678/1‑150 (MQ=255)
                                                                                                tCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATaa                                                    <  1:106552/150‑1 (MQ=255)
                                                                                                        aTCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGcc                                            <  1:380484/150‑1 (MQ=255)
                                                                                                        aTCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGAACGTGGCGGTAAGTAATGTGACGGGGTTCTTCACGTAAATGCACGGTAATGCCGTCCGCTGCCGGGTGCTCGGCAATAAACGCGGcc                                            <  2:688964/150‑1 (MQ=255)
                                                                                                           tctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGc                                         >  1:1252717/1‑150 (MQ=255)
                                                                                                           tctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGc                                         >  1:1120169/1‑150 (MQ=255)
                                                                                                           tctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGc                                         >  2:1587798/1‑150 (MQ=255)
                                                                                                            ctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCa                                        >  1:1250069/1‑150 (MQ=255)
                                                                                                            ctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCa                                        <  1:72035/150‑1 (MQ=255)
                                                                                                              tCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCAcc                                      >  2:1516580/1‑150 (MQ=255)
                                                                                                                ggTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCgg                                    >  2:782360/1‑150 (MQ=255)
                                                                                                                ggTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCgg                                    >  1:110538/1‑150 (MQ=255)
                                                                                                                     ccGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCg                               <  1:1174068/150‑1 (MQ=255)
                                                                                                                     ccGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACAGGATCCg                               <  2:1604728/150‑1 (MQ=255)
                                                                                                                      cGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCgg                              >  1:1215843/1‑150 (MQ=255)
                                                                                                                       gCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCggg                             >  1:572529/1‑150 (MQ=255)
                                                                                                                       gCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCggg                             >  1:562069/1‑150 (MQ=255)
                                                                                                                       gCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCggg                             <  1:1280750/150‑1 (MQ=255)
                                                                                                                        ccATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGt                            <  2:281305/150‑1 (MQ=255)
                                                                                                                         cATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTa                           <  1:1394784/150‑1 (MQ=255)
                                                                                                                         cATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACCGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGATCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTa                           >  1:1334349/1‑150 (MQ=255)
                                                                                                                             tcCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCg                       <  2:552249/150‑1 (MQ=255)
                                                                                                                             tcCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCg                       >  1:1021963/1‑150 (MQ=255)
                                                                                                                             tcCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTAGCGGTCAGTAATGTGACGACGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCg                       >  2:1054017/1‑150 (MQ=255)
                                                                                                                                     cATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcg               >  2:885435/1‑150 (MQ=255)
                                                                                                                                     cATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcg               >  1:7639/1‑150 (MQ=255)
                                                                                                                                     cATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcg               <  2:1500907/150‑1 (MQ=255)
                                                                                                                                      aTGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgc              >  1:72651/1‑150 (MQ=255)
                                                                                                                                       tGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcg             <  1:198526/150‑1 (MQ=255)
                                                                                                                                       tGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcg             <  1:364364/150‑1 (MQ=255)
                                                                                                                                         tgcgcaTCCTGCGTCAGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcgcg           >  2:972538/6‑150 (MQ=255)
                                                                                                                                         cGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcgcg           >  1:1645146/1‑150 (MQ=255)
                                                                                                                                         cGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcgcg           <  2:78967/150‑1 (MQ=255)
                                                                                                                                            gcaTCCTGCGTCAGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTg        >  2:1398325/3‑150 (MQ=255)
                                                                                                                                            gcaTCCTGCGTCAGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTg        >  2:1011853/3‑150 (MQ=255)
                                                                                                                                            gTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTg        <  2:1120169/150‑1 (MQ=255)
                                                                                                                                             tATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgc       <  2:645294/150‑1 (MQ=255)
                                                                                                                                             tATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgc       <  1:931328/150‑1 (MQ=255)
                                                                                                                                             caTCCTGCGTCAGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgc       >  1:307739/2‑150 (MQ=255)
                                                                                                                                              aTCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgcg      <  1:1655303/150‑1 (MQ=255)
                                                                                                                                               tCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgcgc     <  1:1653291/150‑1 (MQ=255)
                                                                                                                                               tCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgcgc     <  1:774973/150‑1 (MQ=255)
                                                                                                                                                ccAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCa    >  1:813489/1‑150 (MQ=255)
                                                                                                                                                  aGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGc  >  2:1617300/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGC  >  NC_000913/2701390‑2701685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑