BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,247,836	Δ1 bp	5.5%	coding (64/663 nt)	yqjA →	DedA family protein YqjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,247,836	0	C	.	5.5%	372.3 / 13.5	109	coding (64/663 nt)	yqjA	DedA family protein YqjA
Reads supporting (aligned to +/- strand): ref base C (41/62); new base . (4/2); total (45/64)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 111 total aligned reads displayed.

GCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAAC  >  NC_000913/3247687‑3247984

gCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCGTCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATc                                                                                                                                                       >  2:636461/1‑150 (MQ=255)

 cAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATcc                                                                                                                                                      <  1:1006722/150‑1 (MQ=255)

        tACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCgat‑g                                                                                                                                              >  1:469164/1‑150 (MQ=255)

        tACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCgat‑g                                                                                                                                              <  2:1672571/150‑1 (MQ=255)

        tACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCgat‑g                                                                                                                                              >  2:946715/1‑150 (MQ=255)

         aCCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCgat‑ga                                                                                                                                             >  1:1427960/1‑150 (MQ=255)

          ccAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCgat‑gat                                                                                                                                            >  2:1928044/1‑150 (MQ=255)

          ccAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCgat‑gat                                                                                                                                            >  1:852625/1‑150 (MQ=255)

            aaaaCAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGGAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTg                                                                                                                                          >  1:1314425/1‑150 (MQ=255)

               aCAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCa                                                                                                                                       <  2:106002/150‑1 (MQ=255)

                cAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAAT‑CATCGATGGATTGGcca                                                                                                                                      <  1:989561/150‑3 (MQ=255)

                  gCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCAtgt                                                                                                                                    >  1:1813207/1‑150 (MQ=255)

                   cAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCAtgtt                                                                                                                                   <  1:1204299/150‑1 (MQ=255)

                   cAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCAtgtt                                                                                                                                   >  2:1786194/1‑150 (MQ=255)

                      tAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTa                                                                                                                                >  2:1182856/1‑150 (MQ=255)

                        aCATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTAtt                                                                                                                              >  2:1800772/1‑150 (MQ=255)

                         cATTGCGGTAGTGCATCTTTTAATACCAGCGTGGCGTTAACCGATTCACCAGGAATATTGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTAttt                                                                                                                             <  2:880506/150‑1 (MQ=255)

                           ttGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTg                                                                                                                           <  2:883173/150‑1 (MQ=255)

                             gCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTc                                                                                                                         >  1:1185557/1‑150 (MQ=255)

                             gCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTc                                                                                                                         >  1:964139/1‑150 (MQ=255)

                              cGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCt                                                                                                                        >  2:872942/1‑150 (MQ=255)

                                  aGTGCGTCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgtt                                                                                                                    <  1:636461/150‑1 (MQ=255)

                                  aGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgtt                                                                                                                    <  1:202642/150‑1 (MQ=255)

                                  aGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgtt                                                                                                                    <  1:792810/150‑1 (MQ=255)

                                  aGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgtt                                                                                                                    >  2:709150/1‑150 (MQ=255)

                                    tGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgtttg                                                                                                                  <  2:1917682/150‑1 (MQ=255)

                                     gCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgtttgt                                                                                                                 <  2:709134/150‑1 (MQ=255)

                                     gCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgtttgt                                                                                                                 <  2:1596887/150‑1 (MQ=255)

                                      cATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTa                                                                                                                <  1:477581/150‑1 (MQ=255)

                                        tCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAAt                                                                                                              >  1:282173/1‑150 (MQ=255)

                                             tAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGt                                                                                                         <  2:221619/150‑1 (MQ=255)

                                                aaCCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTc                                                                                                       >  1:1988656/1‑149 (MQ=255)

                                                aaCCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTc                                                                                                       <  2:1988656/149‑1 (MQ=255)

                                                 aCCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGTCCAATCCATCGAT‑TATTGGGATGTTGTATTTTGTCTTTTTTGTTATTTTGTTcgt                                                                                                     <  2:726674/150‑3 (MQ=255)

                                                  ccAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgt                                                                                                                     <  2:783770/133‑1 (MQ=255)

                                                  ccAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCttgt                                                                                                                     >  1:783770/1‑133 (MQ=255)

                                                   cAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTg                                                                                                   <  1:576017/150‑1 (MQ=255)

                                                    aGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGa                                                                                                  <  2:1978002/150‑1 (MQ=255)

                                                      cGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGaaa                                                                                                <  1:590981/150‑1 (MQ=255)

                                                       gTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAAt                                                                                                              >  1:1667268/1‑135 (MQ=255)

                                                       gTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAAt                                                                                                              <  2:1667268/135‑1 (MQ=255)

                                                         ggCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACg                                                                                             >  1:1046997/1‑150 (MQ=255)

                                                           cGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGgc                                                                                           <  2:282173/150‑1 (MQ=255)

                                                             ttAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGgctt                                                                                         <  2:852625/150‑1 (MQ=255)

                                                                  cGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTc                                                                                    <  1:1359275/150‑1 (MQ=255)

                                                                         ccAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCggcggc                                                                             >  1:178402/1‑150 (MQ=255)

                                                                         ccAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCggcggc                                                                             <  1:1182856/150‑1 (MQ=255)

                                                                            ggAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCtt                                                                          >  1:242774/1‑150 (MQ=255)

                                                                            ggAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTAGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCtt                                                                          <  2:469164/150‑1 (MQ=255)

                                                                                  atgaatgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCcgg                                                                                 <  1:1535976/137‑1 (MQ=255)

                                                                                  atgaatgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCcgg                                                                                 >  2:1927723/1‑137 (MQ=255)

                                                                                  atgaatgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCcgg                                                                                 >  2:1535976/1‑137 (MQ=255)

                                                                                  atgaatgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCcgg                                                                                 <  1:1927723/137‑1 (MQ=255)

                                                                                     aatgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCAGGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCggg                                                                 <  1:1930280/150‑1 (MQ=255)

                                                                                       tgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCg                                                               <  1:1504053/150‑1 (MQ=255)

                                                                                        ggAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGa                                                              >  2:1962153/1‑150 (MQ=255)

                                                                                                 gACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGGGTTGTTTGTAATTTTGTTCGTTGAAAACGGCTTGGTTCCGGCGGCCTTTTTACCGGGCGACAGTTTagt                                                     >  2:374602/1‑148 (MQ=255)

                                                                                                   cccAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGTATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTgg                                                   >  2:1687332/1‑150 (MQ=255)

                                                                                                   cccAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTgg                                                   <  2:932427/150‑1 (MQ=255)

                                                                                                     cAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTa                                                 <  2:835071/150‑1 (MQ=255)

                                                                                                     cAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTa                                                 >  1:1121510/1‑150 (MQ=255)

                                                                                                       aTTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTAtt                                               <  2:95882/150‑1 (MQ=255)

                                                                                                         tgctgcAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTgg                                             <  1:652985/150‑1 (MQ=255)

                                                                                                            tgcAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGtt                                                         >  2:1764974/1‑135 (MQ=255)

                                                                                                            tgcAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGtt                                                         <  1:1764974/135‑1 (MQ=255)

                                                                                                            tgcAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCg                                          <  2:242774/150‑1 (MQ=255)

                                                                                                             gcAAGCCCTGTGGGCGTAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCgg                                         <  2:1314425/150‑1 (MQ=255)

                                                                                                             gcAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCgg                                         <  1:37644/150‑1 (MQ=255)

                                                                                                             gcAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCgg                                         >  2:1826563/1‑150 (MQ=255)

                                                                                                               aaGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCg                                       <  2:528727/150‑1 (MQ=255)

                                                                                                                 gCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCgtg                                     <  2:1046997/150‑1 (MQ=255)

                                                                                                                 gCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCgtg                                     <  1:1099260/150‑1 (MQ=255)

                                                                                                                  cccTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCTTCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCgtgt                                    <  1:1490732/150‑1 (MQ=255)

                                                                                                                   ccTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGtt                                   <  2:1598664/150‑1 (MQ=255)

                                                                                                                   ccTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGtt                                   <  1:1826563/150‑1 (MQ=255)

                                                                                                                    cTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCTTGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTg                                  <  1:772756/150‑1 (MQ=255)

                                                                                                                    cTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTg                                  <  2:1935471/150‑1 (MQ=255)

                                                                                                                      gtgGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGAt                                <  1:449750/150‑1 (MQ=255)

                                                                                                                         ggCGCAGGATTTTGAAACCCTGGGCAATCCATCGAT‑GATTGGCATGTTGTATTTTTTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGc                             <  2:725747/150‑1 (MQ=255)

                                                                                                                         ggCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGc                             <  1:466728/150‑1 (MQ=255)

                                                                                                                          gcgcAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCg                            <  2:1593407/150‑1 (MQ=255)

                                                                                                                              aGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAg                        <  1:1928044/150‑1 (MQ=255)

                                                                                                                               ggATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAgg                       >  1:1519111/1‑150 (MQ=255)

                                                                                                                                   tttGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGt                                            <  1:1885654/125‑1 (MQ=255)

                                                                                                                                   tttGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGt                                            >  2:1885654/1‑125 (MQ=255)

                                                                                                                                   tttGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGcgcg                   <  2:1121510/150‑1 (MQ=255)

                                                                                                                                     tGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGAt                 >  2:182414/1‑150 (MQ=255)

                                                                                                                                     tGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGAt                 >  2:1152936/1‑150 (MQ=255)

                                                                                                                                      gAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATg                >  2:1998481/1‑150 (MQ=255)

                                                                                                                                      gAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGGGTTGATTGCGAAAGGCGCGATg                <  2:685861/150‑1 (MQ=255)

                                                                                                                                       aaaCCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATgg               <  2:1813207/150‑1 (MQ=255)

                                                                                                                                       aaaCCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATgg               <  1:1800772/150‑1 (MQ=255)

                                                                                                                                        acaacatgCCAAT‑CATCGATGGATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATggg              >  2:1460588/8‑150 (MQ=255)

                                                                                                                                        acaacatgCCAAT‑CATCGATGGATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATggg              >  1:1655338/8‑150 (MQ=255)

                                                                                                                                         aCCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGc             <  1:1031792/150‑1 (MQ=255)

                                                                                                                                           ccTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTa           >  1:602586/1‑150 (MQ=255)

                                                                                                                                            catgCCAAT‑CATCGATGGATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGATAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTAt          >  1:1885948/4‑150 (MQ=255)

                                                                                                                                            cTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTAt          >  2:1186426/1‑150 (MQ=255)

                                                                                                                                              ggCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATcc        >  2:74684/1‑150 (MQ=255)

                                                                                                                                                    tCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAAc  <  1:182414/150‑1 (MQ=255)

GCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAAC  >  NC_000913/3247687‑3247984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`