Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 259,130 T→A 5.4% Y324F (TAT→TTT)  phoE ← outer membrane porin PhoE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913259,1300TA 5.4% 301.8 / 5.3 103Y324F (TAT→TTT) phoEouter membrane porin PhoE
Reads supporting (aligned to +/- strand):  ref base T (46/51);  new base A (4/2);  total (50/53)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 106 total aligned reads displayed.

CGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAGTCAAACT  >  NC_000913/258982‑259277
                                                                                                                                                    |                                                                                                                                                   
cGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTtata                                                                                                                                                    >  2:563530/1‑150 (MQ=255)
cGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTtata                                                                                                                                                    >  1:1614346/1‑150 (MQ=255)
 gAGCCTGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATaa                                                                                                                                                   <  1:169030/150‑1 (MQ=255)
 gAGCCGGTGAAGTTAATGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATaa                                                                                                                                                   <  2:465993/150‑1 (MQ=255)
 gAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATaa                                                                                                                                                   <  2:83390/150‑1 (MQ=255)
 gAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATaa                                                                                                                                                   <  2:76233/150‑1 (MQ=255)
    ccGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATca                                                                                                                                                <  2:611354/150‑1 (MQ=255)
          aaGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAAc                                                                                                                                          <  2:1558984/150‑1 (MQ=255)
          aaGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAAc                                                                                                                                          <  2:1083851/150‑1 (MQ=255)
              tAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTg                                                                                                                                      >  2:214491/1‑150 (MQ=255)
               aaCGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGa                                                                                                                                     >  1:1543179/1‑150 (MQ=255)
               aaCGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGa                                                                                                                                     <  2:1489839/150‑1 (MQ=255)
                aCGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGAc                                                                                                                                    <  1:1632409/150‑1 (MQ=255)
                    cGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACatat                                                                                                                                >  2:408233/1‑150 (MQ=255)
                      tgagtgaAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATAttt                                                                                                                              >  1:918862/1‑150 (MQ=255)
                         gtgaAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTttg                                                                                                                           >  1:282835/1‑150 (MQ=255)
                         gtgaAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTttg                                                                                                                           >  1:306537/1‑150 (MQ=255)
                            aaaTGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTttgttg                                                                                                                        <  1:1552132/150‑1 (MQ=255)
                               tgtgCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGaaa                                                                                                                     <  2:1422230/150‑1 (MQ=255)
                                gtgCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAaat                                                                                                                    <  1:1851818/150‑1 (MQ=255)
                                gtgCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAaat                                                                                                                    <  1:40919/150‑1 (MQ=255)
                                  gCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTttgt                                                                                                                          <  2:1937851/142‑1 (MQ=255)
                                  gCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTttgt                                                                                                                          >  1:1937851/1‑142 (MQ=255)
                                  gCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAaataa                                                                                                                  >  1:938988/1‑150 (MQ=255)
                                    cGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAataata                                                                                                                <  1:214491/150‑1 (MQ=255)
                                    cGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTATAAAATcaaccaac                                                                                                                                          <  1:38892/124‑1 (MQ=255)
                                     ggATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATCAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATAc                                                                                                               >  1:1371895/1‑150 (MQ=255)
                                      gATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACg                                                                                                              <  2:938988/150‑1 (MQ=255)
                                        tGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTa                                                                                                            <  2:1715747/150‑1 (MQ=255)
                                           aTCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCa                                                                                                         >  1:105811/1‑150 (MQ=255)
                                           aTCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCa                                                                                                         >  1:1553241/1‑150 (MQ=255)
                                           aTCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCa                                                                                                         >  2:1186135/1‑150 (MQ=255)
                                           aTCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCa                                                                                                         >  1:1767759/1‑150 (MQ=255)
                                               caTCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGa                                                                                                     >  2:197853/1‑150 (MQ=255)
                                               caTCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGa                                                                                                     >  1:1639082/1‑150 (MQ=255)
                                               caTCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGa                                                                                                     >  1:798568/1‑150 (MQ=255)
                                                 tCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATAc                                                                                                               <  1:1335299/138‑1 (MQ=255)
                                                 tCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATAc                                                                                                               >  2:1335299/1‑138 (MQ=255)
                                                   cGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTc                                                                                                 >  1:365256/1‑150 (MQ=255)
                                                    ggCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCg                                                                                                >  1:1188056/1‑150 (MQ=255)
                                                     gCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGa                                                                                               >  1:1896978/1‑150 (MQ=255)
                                                      cAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGat                                                                                              <  2:1614346/150‑1 (MQ=255)
                                                         tatTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGAtata                                                                                           <  2:267161/150‑1 (MQ=255)
                                                             cATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTg                                                                                       <  1:1469769/150‑1 (MQ=255)
                                                             cATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTg                                                                                       >  2:804118/1‑150 (MQ=255)
                                                             cATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTg                                                                                       <  1:693423/150‑1 (MQ=255)
                                                             cATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTg                                                                                       <  2:1035885/150‑1 (MQ=255)
                                                                tAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGAcc                                                                                    <  1:859176/150‑1 (MQ=255)
                                                                 aaaaCTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCa                                                                                   >  1:400323/1‑150 (MQ=255)
                                                                 aaaaCTGATAAATCATGAAAACACCTAAAATATAATAATTAATAATATTCAATATCTAATAGCTATCCATATGATTGATTTAAAAATAAAAAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCa                                                                                   >  2:1051290/1‑150 (MQ=255)
                                                                  aaaCTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAg                                                                                  <  2:798568/150‑1 (MQ=255)
                                                                   aaCTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGa                                                                                 >  2:821140/1‑150 (MQ=255)
                                                                     cTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATc                                                                               <  2:365256/150‑1 (MQ=255)
                                                                        aTACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTtc                                                                            <  2:1101504/150‑1 (MQ=255)
                                                                           cGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTtcatc                                                                         <  2:197478/150‑1 (MQ=255)
                                                                           cGTCATGCCAACCGCGAAAAAATCATAAATATTAATATTCAATTTGTTATCGCTATCCAGTTGCTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACAGACGTCGATATAAATGACCATATCTtaata                                                                         >  2:44178/1‑149 (MQ=255)
                                                                            gTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTtcatca                                                                        <  2:1764037/150‑1 (MQ=255)
                                                                              cATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCAcc                                                                      <  2:1188056/150‑1 (MQ=255)
                                                                                tGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGa                                                                    >  2:748054/1‑150 (MQ=255)
                                                                                  ccAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATa                                                                  <  2:1795410/150‑1 (MQ=255)
                                                                                      ccGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCtt                                                              >  1:1671796/1‑150 (MQ=255)
                                                                                          gACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAat                                                          <  1:563530/150‑1 (MQ=255)
                                                                                           aCAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGAAGTCGATATAATTGACCAGATCTTCATCAACGATACCTTCAata                                                         >  1:658759/1‑150 (MQ=255)
                                                                                              atatCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCt                                                      >  2:1924406/1‑150 (MQ=255)
                                                                                               tatCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCtt                                                     <  2:306537/150‑1 (MQ=255)
                                                                                               tatCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCtt                                                     <  1:1347926/150‑1 (MQ=255)
                                                                                                 tcatcaTTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTc                                                   <  2:439259/150‑1 (MQ=255)
                                                                                                   atcatTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTccc                                                 <  2:282835/150‑1 (MQ=255)
                                                                                                     aattataAATATTCAATTTGTTATCTCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCtt                                               <  2:1371895/149‑1 (MQ=255)
                                                                                                       ttattaATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTc                                             <  1:408233/150‑1 (MQ=255)
                                                                                                       ttattaATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTc                                             <  2:178101/150‑1 (MQ=255)
                                                                                                           taATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATa                                         <  1:456046/150‑1 (MQ=255)
                                                                                                                ttCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACa                                    <  1:1762075/150‑1 (MQ=255)
                                                                                                                  cAATTTGTTATGGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATACTTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACAtg                                  <  1:979077/150‑2 (MQ=255)
                                                                                                                  cAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATa                                  >  1:161749/1‑150 (MQ=255)
                                                                                                                  cAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATa                                  >  2:912383/1‑150 (MQ=255)
                                                                                                                  cAATTTGTTATCGCTAACCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATa                                  >  2:1427431/1‑150 (MQ=255)
                                                                                                                  aaaTTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACAtg                                  <  1:971897/149‑2 (MQ=255)
                                                                                                                        gTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATACCCCAg                            >  2:1487648/1‑150 (MQ=255)
                                                                                                                          tATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCg                          >  2:1256481/1‑150 (MQ=255)
                                                                                                                          tATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCg                          <  2:400323/150‑1 (MQ=255)
                                                                                                                            tCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGAt                        <  2:1639082/150‑1 (MQ=255)
                                                                                                                             cGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATg                       <  2:1958778/150‑1 (MQ=255)
                                                                                                                             cGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATg                       <  1:1186135/150‑1 (MQ=255)
                                                                                                                               cTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTc                                             <  1:564719/126‑1 (MQ=255)
                                                                                                                               cTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTc                                             >  2:564719/1‑126 (MQ=255)
                                                                                                                                 aTCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACg                   >  1:747949/1‑150 (MQ=255)
                                                                                                                                  cagcgtttgttgATTATAAAATCAACCAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGc                  >  2:1936861/5‑150 (MQ=255)
                                                                                                                                   ccAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCa                 >  2:1034891/1‑150 (MQ=255)
                                                                                                                                     aGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGa               <  2:1766638/150‑1 (MQ=255)
                                                                                                                                       tttgttgATTATAAAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGAcc             >  2:1822239/4‑150 (MQ=255)
                                                                                                                                          gttgATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCaaa          >  1:858629/1‑150 (MQ=255)
                                                                                                                                          gttgATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCaa           <  2:246082/149‑1 (MQ=255)
                                                                                                                                          gttgATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCaa           >  1:246082/1‑149 (MQ=255)
                                                                                                                                           ttgATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAg         >  1:506493/1‑150 (MQ=255)
                                                                                                                                            tgATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAGt        >  2:1382944/1‑150 (MQ=255)
                                                                                                                                            tgATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAGt        >  1:308370/1‑150 (MQ=255)
                                                                                                                                               ttttATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAGTCaa     >  2:1596372/1‑150 (MQ=255)
                                                                                                                                               ttttATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAGTCaa     >  1:1827889/1‑150 (MQ=255)
                                                                                                                                                  tataATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAGTCAAACt  <  1:1172459/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACATCCGGCAATATTCATTAAAACTGATACGTCATGCCAACCGCGACAATATCATCATTATTAATATTCAATTTGTTATCGCTATCCAGTTGGTTGATTTTATAATCAACAAACGCTGACATATTTTTGTTGAAATAATACGTAGCACCGACGTCGATATAATTGACCAGATCTTCATCACCGATACCTTCAATATCTTTCCCTTTCGATAAGACATAACCCAGCGATGGACGCAGACCAAAGTCAAACT  >  NC_000913/258982‑259277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑