Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,563,266 A→T 5.1% intergenic (+149/‑326) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein YffP/CPZ‑55 prophage; uncharacterized protein YffQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,563,2660AT 5.1% 241.2 / 2.9 79intergenic (+149/‑326)yffP/yffQCPZ‑55 prophage; uncharacterized protein YffP/CPZ‑55 prophage; uncharacterized protein YffQ
Reads supporting (aligned to +/- strand):  ref base A (43/32);  new base T (2/2);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01

CGGACAAACGCGCGCGTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAG  >  NC_000913/2563123‑2563415
                                                                                                                                               |                                                                                                                                                     
cGGACAAACGCGCGCGTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTAATCGAAAAATCCCTCTCAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTAc                                                                                                                                                 >  2:427820/1‑150 (MQ=255)
  gACAAACGCGCGCGTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTAccc                                                                                                                                               >  2:1025471/1‑150 (MQ=255)
  gACAAACGCGCGCGTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTAccc                                                                                                                                               >  2:691516/1‑150 (MQ=255)
    cAAACGCGCGCGTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCgg                                                                                                                                             >  2:898657/1‑150 (MQ=255)
       aCGCGCGCGTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGcc                                                                                                                                          <  2:83890/150‑1 (MQ=255)
             gcgTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTtctc                                                                                                                                    >  1:624641/1‑150 (MQ=255)
             gcgTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTtctc                                                                                                                                    <  2:472431/150‑1 (MQ=255)
                  gCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAAt                                                                                                                               >  2:1774624/1‑150 (MQ=255)
                  gCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAAt                                                                                                                               >  2:552507/1‑150 (MQ=255)
                        gTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGACCTTTCTCACAATCCCCGc                                                                                                                         <  2:25053/150‑1 (MQ=255)
                          cAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTg                                                                                                                       <  1:552507/150‑1 (MQ=255)
                             cGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACa                                                                                                                    >  1:1010817/1‑150 (MQ=255)
                                cAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATta                                                                                                                 >  1:392883/1‑150 (MQ=255)
                                cAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATta                                                                                                                 <  1:231768/150‑1 (MQ=255)
                                      tGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCAc                                                                                                           <  1:1661461/150‑1 (MQ=255)
                                       ggCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCAcc                                                                                                          >  2:1959596/1‑150 (MQ=255)
                                         cGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCa                                                                                                        >  1:1323298/1‑150 (MQ=255)
                                           gTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATc                                                                                                      <  2:199431/150‑1 (MQ=255)
                                             cATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTtctc                                                                                                                                    <  2:1075427/118‑1 (MQ=255)
                                             cATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTtctc                                                                                                                                    >  1:1075427/1‑118 (MQ=255)
                                               tttCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTc                                                                                                  >  1:164124/1‑150 (MQ=255)
                                                ttCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTcc                                                                                                 <  1:898657/150‑1 (MQ=255)
                                                  ccccGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGt                                                                                               <  1:1879312/150‑1 (MQ=255)
                                                    ccGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGttt                                                                                             <  1:1774624/150‑1 (MQ=255)
                                                      gCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATcc                                                                                                                             >  1:1796728/1‑116 (MQ=255)
                                                      gCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATcc                                                                                                                             <  2:1796728/116‑1 (MQ=255)
                                                      gCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTc                                                                                           >  1:261223/1‑150 (MQ=255)
                                                         ccTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAgg                                                                                        >  1:1789893/1‑150 (MQ=255)
                                                         ccTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAgg                                                                                        >  2:1372035/1‑150 (MQ=255)
                                                            aCGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCTCGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTgg                                                                                     <  2:375764/150‑1 (MQ=255)
                                                              gcgAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGga                                                                                   <  1:1972765/150‑1 (MQ=255)
                                                               cgAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGgag                                                                                  >  2:1312342/1‑150 (MQ=255)
                                                               cgAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGgag                                                                                  <  2:1164976/150‑1 (MQ=255)
                                                                gAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGgaga                                                                                 >  2:1965094/1‑150 (MQ=255)
                                                                 aaaaaTCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGAc                                                                                <  1:691516/150‑1 (MQ=255)
                                                                   aaaTCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTg                                                                              >  2:71084/1‑150 (MQ=255)
                                                                     aTCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCAcc                                                                                                          >  2:889348/1‑120 (MQ=255)
                                                                     aTCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCAcc                                                                                                          <  1:889348/120‑1 (MQ=255)
                                                                     aTCCCTCACAACTACCCTGCAAAATTTCCAGAATTCAAGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTAAATCACCCATCTTTCCGTTTTCACGTCGGAGACTGAt                                                                            >  2:652644/1‑150 (MQ=255)
                                                                      tCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATg                                                                           >  1:1717354/1‑150 (MQ=255)
                                                                        ccTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGgc                                                                         <  1:1959596/150‑1 (MQ=255)
                                                                          tCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGgccg                                                                       >  2:377048/1‑150 (MQ=255)
                                                                             caACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGccgccg                                                                    <  1:1025471/150‑1 (MQ=255)
                                                                                 tACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGt                                                                <  1:1350844/150‑1 (MQ=255)
                                                                                     cTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGCTGGCCGCCGACGTTGca                                                            >  2:1094627/1‑150 (MQ=255)
                                                                                     cTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGca                                                            >  2:1087496/1‑150 (MQ=255)
                                                                                       gCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGcaca                                                          >  2:1975384/1‑150 (MQ=255)
                                                                                       gCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCATTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGcaca                                                          >  2:1333642/1‑150 (MQ=255)
                                                                                        cAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACaa                                                         >  2:63879/1‑150 (MQ=255)
                                                                                           aTTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTg                                                      <  2:392883/150‑1 (MQ=255)
                                                                                              ttCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGAtt                                                   >  1:824411/1‑150 (MQ=255)
                                                                                                  aGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAAtt                                               <  2:217988/150‑1 (MQ=255)
                                                                                                    aTTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTc                                             <  1:1312342/150‑1 (MQ=255)
                                                                                                             acacGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTc                                    <  1:1372035/150‑1 (MQ=255)
                                                                                                               acGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCaa                                  >  1:1836404/1‑150 (MQ=255)
                                                                                                                 gcgcCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAAt                                <  1:1975384/150‑1 (MQ=255)
                                                                                                                 gcgcCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAAt                                >  1:193077/1‑150 (MQ=255)
                                                                                                                  cgcCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATc                               >  1:486869/1‑150 (MQ=255)
                                                                                                                    ccAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCCCAATCCCCGCTGACATTATATCCCCCATCTTTCCGTTTTCAGGTGGGAGACTGAGGGCCGCCGCCGTTGCACAATTGATTAATTTCTGACCATTCAAATCaa                             >  1:1314433/1‑150 (MQ=255)
                                                                                                                    ccAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCaa                             >  2:1528929/1‑150 (MQ=255)
                                                                                                                     cAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAAc                            <  1:377048/150‑1 (MQ=255)
                                                                                                                       gtgagaaaGGCCCGGGTAGGTTGCTAACTGCCTGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTc                          >  1:602148/6‑150 (MQ=38)
                                                                                                                         aaTAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGATGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTccc                        >  1:76527/1‑150 (MQ=255)
                                                                                                                           tAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCccac                      >  1:753931/1‑150 (MQ=255)
                                                                                                                             aaGGCCCGGGTAGGTTGCTAACTGCCTGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCccacca                    <  2:602148/150‑1 (MQ=255)
                                                                                                                               ggCCCGGGTAGGTTGCTAACTGCCTGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGtt                                                               <  2:139930/105‑1 (MQ=37)
                                                                                                                               ggCCCGGGTAGGTTGCTAACTGCCTGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGtt                                                               >  1:139930/1‑105 (MQ=37)
                                                                                                                                gCCCAGGCAGTTAGCAACCTACGTGGGCCTTTCTCAGAATCCCCGCTTACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAg                 <  2:1323298/150‑1 (MQ=255)
                                                                                                                                 cccAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGc                >  1:1158739/1‑150 (MQ=255)
                                                                                                                                  ccAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGCCGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGcc               >  1:521954/1‑150 (MQ=255)
                                                                                                                                  ccAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGcc               <  2:568882/150‑1 (MQ=255)
                                                                                                                                       cAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCcaacaa          >  2:554864/1‑150 (MQ=255)
                                                                                                                                       cAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCcaacaa          <  1:1965094/150‑1 (MQ=255)
                                                                                                                                       cAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCCTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTATGAACATTCAAATCAACTCCCACCAAAGCcaacaa          <  1:1333642/150‑1 (MQ=255)
                                                                                                                                           tAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCTGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATg      <  2:1604202/150‑1 (MQ=255)
                                                                                                                                           tAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATg      >  2:426805/1‑150 (MQ=255)
                                                                                                                                            aGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTg                                                      <  1:1461295/101‑1 (MQ=255)
                                                                                                                                            aGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTg                                                      >  2:1461295/1‑101 (MQ=255)
                                                                                                                                              cAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAACATGGGa   >  2:90382/1‑150 (MQ=255)
                                                                                                                                               aaCCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACACATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAg  >  2:1038142/1‑150 (MQ=255)
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CGGACAAACGCGCGCGTAGCCGACGTCAGCGGCAAGACTGGCGGTCATTTCCCCGCTCCTACGCGAAAAATCCCTCACAACTACCCTGCAAATTTTCCAGATTTCACGTACACGCGCCAAAAATAAAGGCCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAG  >  NC_000913/2563123‑2563415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑