Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,599,244 T→G 8.0% I173L (ATC→CTC)  dapA ← 4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,599,2440TG 8.0% 247.7 / 10.9 86I173L (ATC→CTC) dapA4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base T (37/42);  new base G (3/4);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599096‑2599387
                                                                                                                                                    |                                                                                                                                               
gctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATc                                                                                                                                                >  1:1131340/1‑150 (MQ=255)
 ctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCt                                                                                                                                               <  1:219674/150‑1 (MQ=255)
  tgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAATCCTGCTCTTTGATCTg                                                                                                                                              <  2:488324/150‑1 (MQ=255)
   gcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTgg                                                                                                                                             >  1:711606/1‑150 (MQ=255)
   gcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTgg                                                                                                                                             <  1:422664/150‑1 (MQ=255)
   gcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTgg                                                                                                                                             <  2:83655/150‑1 (MQ=255)
     cAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGtt                                                                                                                                           <  1:1964117/150‑1 (MQ=255)
      aGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGttt                                                                                                                                          >  2:411862/1‑150 (MQ=255)
        tttGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCAAAGAGCTGGtttca                                                                                                                                        <  1:810597/150‑3 (MQ=255)
        tttGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCAAAGAGCTGGtttca                                                                                                                                        <  1:56077/150‑3 (MQ=255)
        tttGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCAAAGAGCTGGtttca                                                                                                                                        <  1:1560766/150‑3 (MQ=255)
          tGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTacac                                                                                                                                      <  2:1620551/150‑1 (MQ=255)
           gCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCAAAGAGCTGGtttcagat                                                                                                                                     <  2:90809/150‑6 (MQ=255)
           gCACATCTGGGCCATATCACGCGCTGAGACGTTAGACTTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTATTTGATCTGGTTTACAcg                                                                                                                                     >  2:173562/1‑150 (MQ=255)
            cacaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgc                                                                                                                                    >  2:1216780/1‑150 (MQ=255)
             acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   <  1:1106895/150‑1 (MQ=255)
              caTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGt                                                                                                                                  >  1:518049/1‑150 (MQ=255)
              caTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGt                                                                                                                                  >  2:868843/1‑150 (MQ=255)
                tCTGTGCCATATCACGCGCTGCGACGTTAGCCGTAAAGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTa                                                                                                                                <  2:1751524/150‑1 (MQ=255)
                tCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTa                                                                                                                                <  2:1887949/150‑1 (MQ=255)
                tCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTa                                                                                                                                <  1:923568/150‑1 (MQ=255)
                tCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTa                                                                                                                                <  2:1806360/150‑1 (MQ=255)
                  tGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCTCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTTGTTTACACGCGTTAAg                                                                                                                              <  2:1018402/150‑1 (MQ=255)
                    ggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGtt                                                                                                                            <  2:104828/150‑1 (MQ=255)
                        atatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCt                                                                                                                        <  1:1976504/150‑1 (MQ=255)
                         tatCACTCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGAATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTg                                                                                                                       <  1:1063020/150‑1 (MQ=255)
                         tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTg                                                                                                                       <  1:1318585/150‑1 (MQ=255)
                          atCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGt                                                                                                                      >  1:60940/1‑150 (MQ=255)
                            cACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTg                                                                                                                    >  2:770562/1‑150 (MQ=255)
                            cACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTg                                                                                                                    <  2:1131340/150‑1 (MQ=255)
                            cACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTg                                                                                                                    <  1:1690394/150‑1 (MQ=255)
                              cgcgCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGcc                                                                                                                  <  1:1277560/150‑1 (MQ=255)
                                cgcTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCctc                                                                                                                >  1:1573957/1‑150 (MQ=255)
                                   tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCttt                                                                                                             <  1:1760411/150‑1 (MQ=255)
                                   tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCttt                                                                                                             >  1:1358123/1‑150 (MQ=255)
                                    gCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTg                                                                                                            <  1:868843/150‑1 (MQ=255)
                                             gCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGata                                                                                                   >  1:951523/1‑150 (MQ=255)
                                                  aaCGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGAGCGGGTTTACACCCGTTAAGTGCCCGGTGGCCTCTTTGATTCCGATAATAtt                                                                                              >  2:698525/1‑150 (MQ=255)
                                                      gAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                          <  1:528739/150‑1 (MQ=255)
                                                       aaaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTa                                                                                         >  1:1050696/1‑150 (MQ=255)
                                                             ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTGGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                   <  2:1587997/150‑1 (MQ=255)
                                                              cccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                                  >  2:161552/1‑150 (MQ=255)
                                                                  tGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGa                                                                              >  2:391129/1‑150 (MQ=255)
                                                                      cGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGc                                                                          <  1:391129/150‑1 (MQ=255)
                                                                       gCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGcc                                                                         <  1:411862/150‑1 (MQ=255)
                                                                          cAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGcc                                                                                 >  2:1344585/1‑139 (MQ=255)
                                                                          cAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGcc                                                                                 <  1:1344585/139‑1 (MQ=255)
                                                                            aTTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAACATCATCTGAACCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGATGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCg                                                                    <  2:414003/150‑1 (MQ=255)
                                                                            aTTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCg                                                                    >  2:210577/1‑150 (MQ=255)
                                                                               gCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                                 >  2:232229/1‑150 (MQ=255)
                                                                                 aTGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGGTAAGTTCCCTGTTGCCTCTTTGAGTCCGATAATATTTTTTACTTTCGCCAGCCGGCCCACCGTTTcc                                                               >  1:1608778/1‑150 (MQ=255)
                                                                                 aTGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTGTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTcc                                                               >  1:1615687/1‑150 (MQ=255)
                                                                                  tGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCg                                                              <  1:770562/150‑1 (MQ=255)
                                                                                      gTCCAGCGCGCTCTAATCATCTCCGCTCATGATAACAAAATGATCTGAAAGCAGCTCTTTGATCTGGTTTACACGCGTTAATTTCCCTGTTGCCTCTTTGATTCCTATAATATTTTTTAGTTTCGCCAGACGGCCCACAGTTTCCGGGAg                                                          <  2:60940/150‑1 (MQ=255)
                                                                                      gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAg                                                          >  2:384107/1‑150 (MQ=255)
                                                                                      gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAg                                                          >  1:701817/1‑150 (MQ=255)
                                                                                        ccAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCa                                                        >  2:77978/1‑150 (MQ=255)
                                                                                        ccAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCa                                                        >  1:1354699/1‑150 (MQ=255)
                                                                                               gcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCa                                                 <  1:161552/150‑1 (MQ=255)
                                                                                                 tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                               <  1:118653/150‑1 (MQ=255)
                                                                                                  cGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGcc                                              >  2:1164032/1‑150 (MQ=255)
                                                                                                    catcatCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAg                                            <  1:1274683/150‑1 (MQ=255)
                                                                                                    catcatCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAg                                            >  1:67908/1‑150 (MQ=255)
                                                                                                           gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                     >  2:1771020/1‑150 (MQ=255)
                                                                                                              gcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACgg                                  <  1:173562/150‑1 (MQ=255)
                                                                                                               cTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGc                                 <  2:518049/150‑1 (MQ=255)
                                                                                                                   gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAt                             >  1:1017563/1‑150 (MQ=255)
                                                                                                                    cagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAtt                            <  2:711606/150‑1 (MQ=255)
                                                                                                                      gAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtat                          >  2:285440/1‑150 (MQ=255)
                                                                                                                        aCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCTCAGCCAGTACGGGACGGCACATtataa                        >  1:1312799/1‑149 (MQ=255)
                                                                                                                        aCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATAc                        <  2:1573957/150‑1 (MQ=255)
                                                                                                                         cAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGACAGTACGGGACGGCACATTATACa                       >  1:808721/1‑150 (MQ=255)
                                                                                                                           aaaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGa                     <  2:707169/150‑1 (MQ=255)
                                                                                                                               gcgtgtaaaCCAGATCAAAGAGCTGGTTTCCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAttt                 >  2:109588/7‑150 (MQ=255)
                                                                                                                                cgtgtaaaCCAGATCAAAGAGCTGGTTTACACGAGTTAAGTTCCCTGTTGCCTCTTTGATTCAGATAATATTTTTTACTTTCGCCAGAAGGCCACCCGTTTCAGTGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                >  2:1876136/6‑150 (MQ=255)
                                                                                                                                 tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGc               <  1:232229/150‑1 (MQ=255)
                                                                                                                                  cTGAAACCAGCTCTTTGATCTGGTTTACCCGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGCCGGCACATTATACAGAATTTGCg              >  2:1871003/1‑150 (MQ=255)
                                                                                                                                  cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCg              <  1:1216780/150‑1 (MQ=255)
                                                                                                                                    gAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGc            <  1:384107/150‑1 (MQ=255)
                                                                                                                                    gAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGc            <  1:77978/150‑1 (MQ=255)
                                                                                                                                       aCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTAGTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACTGTACGGCACATTATACAGAATTTGCGGCAgg         <  2:1203880/150‑1 (MQ=255)
                                                                                                                                       aCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg         >  2:1537741/1‑150 (MQ=255)
                                                                                                                                        ccAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGt        >  2:1521981/1‑150 (MQ=255)
                                                                                                                                           gCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAg     >  2:372256/1‑150 (MQ=255)
                                                                                                                                             tctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTa   >  2:217680/1‑150 (MQ=255)
                                                                                                                                              ctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAt  >  1:968342/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599096‑2599387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑