Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 282,677 C→T 5.9% P134S (CCG→TCG)  yagE → CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913282,6770CT 5.9% 316.7 / 4.1 99P134S (CCG→TCG) yagECP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase
Reads supporting (aligned to +/- strand):  ref base C (54/39);  new base T (4/2);  total (58/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTC  >  NC_000913/282530‑282826
                                                                                                                                                   |                                                                                                                                                     
aCCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCg                                                                                                                                                     <  1:1134031/150‑1 (MQ=255)
 ccAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCgg                                                                                                                                                    >  2:1579128/1‑150 (MQ=255)
    aCGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGa                                                                                                                                                 <  1:168884/150‑1 (MQ=255)
     cGCCCGGGAATCCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGAt                                                                                                                                                <  1:1611681/150‑1 (MQ=255)
     cGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGAt                                                                                                                                                <  1:1390793/150‑1 (MQ=255)
      gCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGTGTCACGCTGCCGGTGATg                                                                                                                                               <  2:322131/150‑1 (MQ=255)
       cccGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGc                                                                                                                                              >  2:1121722/1‑150 (MQ=255)
        ccGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGct                                                                                                                                             >  2:1770841/1‑150 (MQ=255)
         cGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGTAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGTTGATGctc                                                                                                                                            <  2:859274/150‑1 (MQ=255)
         cGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGctc                                                                                                                                            <  1:1121722/150‑1 (MQ=255)
          gggAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGctct                                                                                                                                           >  2:1569477/1‑150 (MQ=255)
            gAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCtat                                                                                                                                         <  2:1141579/150‑1 (MQ=255)
              aaCCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATaa                                                                                                                                       <  1:728025/150‑1 (MQ=255)
                ccATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGctc                                                                                                                                            <  1:678983/143‑1 (MQ=255)
                ccATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGctc                                                                                                                                            >  2:678983/1‑143 (MQ=255)
                 cATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGCCAGCGTCACGCTGCCGGTGATGCTCTATAACtt                                                                                                                                    >  2:414076/1‑150 (MQ=255)
                 cATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACtt                                                                                                                                    >  1:423420/1‑150 (MQ=255)
                  aTCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTc                                                                                                                                   >  2:1195899/1‑150 (MQ=255)
                  aTCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTc                                                                                                                                   >  1:1977180/1‑150 (MQ=255)
                    cGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTccc                                                                                                                                 <  2:1977180/150‑1 (MQ=255)
                    cGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTccc                                                                                                                                 >  1:478263/1‑150 (MQ=255)
                    cGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTccc                                                                                                                                 <  1:439959/150‑1 (MQ=255)
                     gAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCg                                                                                                                                >  2:1732130/1‑150 (MQ=255)
                      aaCTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCgg                                                                                                                               <  2:423420/150‑1 (MQ=255)
                       aCTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGgc                                                                                                                              <  2:1990097/150‑1 (MQ=255)
                       aCTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGgc                                                                                                                              <  2:1240900/150‑1 (MQ=255)
                        cTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGT‑ACCCGTCCGGTGATGCTCGATTACATACCCgcgc                                                                                                                            >  2:1564879/1‑150 (MQ=255)
                          cagccagcACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCt                                                                                                                           >  2:1251685/1‑150 (MQ=255)
                            gccagcACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGa                                                                                                                         >  2:622059/1‑150 (MQ=255)
                            gccagcACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGa                                                                                                                         <  1:1578188/150‑1 (MQ=255)
                               agcACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCg                                                                                                                      >  2:391682/1‑150 (MQ=255)
                                gcACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCgg                                                                                                                     >  1:1784967/1‑150 (MQ=255)
                                   cgcgCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCa                                                                                                                  >  1:350623/1‑150 (MQ=255)
                                      gcagcaGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGa                                                                                                               <  1:1438532/150‑1 (MQ=255)
                                        agcagGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATc                                                                                                             <  2:1784967/150‑1 (MQ=255)
                                         gcagGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCt                                                                                                            <  1:1770841/150‑1 (MQ=255)
                                         gcagGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCt                                                                                                            <  2:218201/150‑1 (MQ=255)
                                               gggcgCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTcc                                                                                                      >  2:1699486/1‑150 (MQ=255)
                                                ggcgCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCg                                                                                                     >  1:618362/1‑150 (MQ=255)
                                                        cGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGt                                                                                             >  2:1199167/1‑150 (MQ=255)
                                                          gCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGa                                                                                           >  2:63452/1‑150 (MQ=255)
                                                              cGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATc                                                                                                             <  1:1538885/128‑1 (MQ=255)
                                                              cGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATc                                                                                                             >  2:1538885/1‑128 (MQ=255)
                                                              cGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAAc                                                                                       <  1:391682/150‑1 (MQ=255)
                                                                   tgATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCg                                                                                  >  1:1971891/1‑150 (MQ=255)
                                                                    gATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCCGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGc                                                                                 <  1:996555/150‑1 (MQ=255)
                                                                     aTCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGcc                                                                                <  2:187775/150‑1 (MQ=255)
                                                                        aaCCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTc                                                                                                                                   >  2:1184867/1‑96 (MQ=255)
                                                                        aaCCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTc                                                                                                                                   <  1:1184867/96‑1 (MQ=255)
                                                                         aCCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACt                                                                            >  1:1232220/1‑150 (MQ=255)
                                                                             ctactaCTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTcgcg                                                                        <  1:1455132/150‑1 (MQ=255)
                                                                                 tactGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCgcagc                                                                    <  2:1971891/150‑1 (MQ=255)
                                                                                 tactGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCgcagc                                                                    >  1:694213/1‑150 (MQ=255)
                                                                                       aaaGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAAtatt                                                              >  1:1566518/1‑150 (MQ=255)
                                                                                       aaaGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAAtatt                                                              >  1:1029126/1‑150 (MQ=255)
                                                                                         aGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAAtattat                                                            >  1:1245454/1‑150 (MQ=255)
                                                                                            gtCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCgg                                                         >  1:1908260/1‑150 (MQ=255)
                                                                                             tCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCCACTCGCGCAGCAATATTATCGGc                                                        >  2:1290736/1‑150 (MQ=255)
                                                                                              cGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCgcgc                                                                       <  1:1559022/134‑1 (MQ=255)
                                                                                              cGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCgcgc                                                                       <  1:1566420/134‑1 (MQ=255)
                                                                                              cGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCgcgc                                                                       >  2:1566420/1‑134 (MQ=255)
                                                                                              cGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCgcgc                                                                       >  2:1559022/1‑134 (MQ=255)
                                                                                              cGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCa                                                       >  2:917823/1‑150 (MQ=255)
                                                                                               ggAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCAt                                                      >  1:541934/1‑150 (MQ=255)
                                                                                               ggAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCAt                                                      >  1:1171357/1‑150 (MQ=255)
                                                                                                    cGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAg                                                 <  1:1732130/150‑1 (MQ=255)
                                                                                                      aaCCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTCAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGac                                               <  1:1199167/150‑1 (MQ=255)
                                                                                                          tGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTg                                                                                                           >  2:1838658/1‑86 (MQ=255)
                                                                                                          tGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTg                                                                                                           <  1:1838658/86‑1 (MQ=255)
                                                                                                          tGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCa                                           >  2:94857/1‑150 (MQ=255)
                                                                                                          tGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCa                                           <  1:917823/150‑1 (MQ=255)
                                                                                                          tGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCa                                           >  1:1164697/1‑150 (MQ=255)
                                                                                                            aTCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATc                                         >  2:1153234/1‑150 (MQ=255)
                                                                                                            aTCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATc                                         >  1:1633927/1‑150 (MQ=255)
                                                                                                             tCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCg                                        >  1:291588/1‑150 (MQ=255)
                                                                                                              ccGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGa                                       >  1:1168488/1‑150 (MQ=255)
                                                                                                                 cTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTc                                    <  1:748149/150‑1 (MQ=255)
                                                                                                                     ttCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTc                                >  2:1951354/1‑150 (MQ=255)
                                                                                                                       cGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGc                              >  1:236410/1‑150 (MQ=255)
                                                                                                                         aGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGccc                            >  1:745675/1‑150 (MQ=255)
                                                                                                                          gCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCa                           <  2:1106711/150‑1 (MQ=255)
                                                                                                                             ggTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCt                        >  2:473992/1‑150 (MQ=255)
                                                                                                                                ggCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATA‑TATAGGCATCAAAGACACCATCGACAACGTCGCCCCCCAgcgc                    >  1:762220/1‑150 (MQ=255)
                                                                                                                                caccGGCAGCGTGACGCTGTCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTgcg                     >  2:279360/3‑150 (MQ=255)
                                                                                                                                caccGGCAGCGTGACGCTGTCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTgcg                     >  1:755453/3‑150 (MQ=255)
                                                                                                                                  ccGGCAGCGTGACGCTGTCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTcg                                                                          >  1:798325/1‑95 (MQ=255)
                                                                                                                                  ccGGCAGCGTGACGCTGTCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTcg                                                                          >  1:791679/1‑95 (MQ=255)
                                                                                                                                  ccGGCAGCGTGACGCTGTCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTcg                                                                          <  2:798325/95‑1 (MQ=255)
                                                                                                                                  ccGGCAGCGTGACGCTGTCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTcg                                                                          <  2:791679/95‑1 (MQ=255)
                                                                                                                                  ccGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCgca                   >  1:541673/1‑150 (MQ=255)
                                                                                                                                     aCAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCgcagca                <  2:478263/150‑1 (MQ=255)
                                                                                                                                     aCAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCgcagca                <  2:350623/150‑1 (MQ=255)
                                                                                                                                        gCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGa             >  2:1842795/1‑150 (MQ=255)
                                                                                                                                          gTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATc           >  1:1944086/1‑150 (MQ=255)
                                                                                                                                          gTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATc           >  1:597872/1‑150 (MQ=255)
                                                                                                                                           tCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATcc          <  1:1569477/150‑1 (MQ=255)
                                                                                                                                           tCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATcc          >  2:1912893/1‑150 (MQ=255)
                                                                                                                                                cTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATAcc     >  1:1926796/1‑150 (MQ=255)
                                                                                                                                                 tGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCg    <  1:622059/150‑1 (MQ=255)
                                                                                                                                                   ccGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGTCTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTc  <  1:1699486/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTGCCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTGAAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTC  >  NC_000913/282530‑282826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑