Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,856,838 T→A 5.5% intergenic (‑32/‑255) ygbA ← / → mutS protein YgbA/DNA mismatch repair protein MutS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,856,8380TA 5.5% 346.3 / 7.4 109intergenic (‑32/‑255)ygbA/mutSprotein YgbA/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base T (48/55);  new base A (3/3);  total (51/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 115 total aligned reads displayed.

AACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGCTT  >  NC_000913/2856690‑2856986
                                                                                                                                                    |                                                                                                                                                    
aacaaacaACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATAtt                                                                                                                                                     >  2:1614750/1‑150 (MQ=255)
 acaaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTa                                                                                                                                                    <  2:90630/150‑1 (MQ=255)
  caaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATAttta                                                                                                                                                   >  2:480166/1‑148 (MQ=255)
      caacGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatct                                                                                                                                               <  1:1639376/150‑1 (MQ=255)
       aacGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatctt                                                                                                                                              >  1:391501/1‑150 (MQ=255)
          tccTCGTAATGCTCCGTCTCCGCTGACGCCTGCGGGCATTTCGCTTGTTAAAGATCGATCATTTTTTTAATCTTCAGTTTTTCACTAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTAAatcttaat                                                                                                                                           <  2:1340799/149‑1 (MQ=255)
          gCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaat                                                                                                                                           <  1:144076/150‑1 (MQ=255)
           ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                          >  1:1316788/1‑150 (MQ=255)
           ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                          >  1:1748939/1‑150 (MQ=255)
           ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAATATAAATACatcttaatg                                                                                                                                          <  2:313727/150‑2 (MQ=255)
             tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                        <  2:1104760/150‑1 (MQ=255)
                tAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAATATAAATACatcttaatgaggtg                                                                                                                                     <  1:801417/150‑7 (MQ=255)
                   tGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                          <  2:1025836/142‑1 (MQ=255)
                   tGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                          >  1:1025836/1‑142 (MQ=255)
                   tGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAggg                                                                                                                                  <  1:1208376/150‑1 (MQ=255)
                   tGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAggg                                                                                                                                  >  1:151499/1‑150 (MQ=255)
                     ctccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCa                                                                                                                                >  1:1883902/1‑150 (MQ=255)
                      tccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCAc                                                                                                                               <  2:1135745/150‑1 (MQ=255)
                       ccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACt                                                                                                                              <  1:1005538/150‑1 (MQ=255)
                         ggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTAt                                                                                                                            >  1:812949/1‑150 (MQ=255)
                            tccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGAc                                                                                                                         <  1:280283/150‑1 (MQ=255)
                             ccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACt                                                                                                                        <  2:1273900/150‑1 (MQ=255)
                               gCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTcc                                                                                                                      <  1:1004794/150‑1 (MQ=255)
                                 tGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACTAGAGATACGCTTGCCGTACATGCTTCCTCCACCTCATTAAGATTTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCaa                                                                                                                    <  1:979159/150‑1 (MQ=255)
                                   aCGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaag                                                                                                                  <  2:508961/150‑1 (MQ=255)
                                   aCGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTAAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaag                                                                                                                  <  2:636509/150‑1 (MQ=255)
                                    cGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaaga                                                                                                                 >  1:769620/1‑150 (MQ=255)
                                      ccTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaag                                                                                                               <  1:1736726/150‑1 (MQ=255)
                                      ccTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaag                                                                                                               <  1:1285151/150‑1 (MQ=255)
                                           gggCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAggg                                                                                                          <  1:1210742/150‑1 (MQ=255)
                                           gggCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAggg                                                                                                          <  2:40110/150‑1 (MQ=255)
                                           gggCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAggg                                                                                                          >  2:1654760/1‑150 (MQ=255)
                                            ggCATTTCGATTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATATTAATCTTAAAGGGAAAAAAGAATACAAAGAAGAAGGGt                                                                                                         >  2:846776/1‑150 (MQ=255)
                                             gCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACTAGAGATACGCATGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGtt                                                                                                        >  1:200544/1‑150 (MQ=255)
                                              cATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTa                                                                                                       <  1:1614750/150‑1 (MQ=255)
                                                  tCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCa                                                                                                   >  1:226032/1‑150 (MQ=255)
                                                    gCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAc                                                                                                 >  1:1732394/1‑150 (MQ=255)
                                                     cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                                <  1:1264528/150‑1 (MQ=255)
                                                     cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                                >  1:235058/1‑150 (MQ=255)
                                                          tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                           <  1:1921443/150‑1 (MQ=255)
                                                             aGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTACACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTACAAAGAAGAAGGGTTAGCCAACCGATACAAt                                                                                        >  2:1210600/1‑150 (MQ=255)
                                                                  gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                                   >  2:913767/1‑150 (MQ=255)
                                                                  gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                                   >  1:1533458/1‑150 (MQ=255)
                                                                   atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                                  <  2:553409/150‑1 (MQ=255)
                                                                    tcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGt                                                                                 <  1:417677/150‑1 (MQ=255)
                                                                      aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTTCCTCCACCTCATTAAGATGTATTTATATTACATATTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTaa                                                                               >  1:761041/1‑149 (MQ=255)
                                                                      aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTAc                                                                               >  2:1969316/1‑150 (MQ=255)
                                                                      aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTAc                                                                               <  2:151499/150‑1 (MQ=255)
                                                                      aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTAc                                                                               >  1:736437/1‑150 (MQ=255)
                                                                      aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTAc                                                                               >  1:768054/1‑150 (MQ=255)
                                                                         tttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTTCCTCCACATCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGAATCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGAACTTg                                                                            >  1:1615362/1‑150 (MQ=255)
                                                                         tttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAGCGATACAATTTTGCGTTCTTg                                                                            >  1:1873009/1‑150 (MQ=255)
                                                                                  gTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAg                                                                   <  2:1165206/150‑1 (MQ=255)
                                                                                    cAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCa                                                                 <  1:1065708/150‑1 (MQ=255)
                                                                                    cAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCa                                                                 <  1:1072612/150‑1 (MQ=255)
                                                                                          ttCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGc                                                           <  2:736437/150‑1 (MQ=255)
                                                                                           tCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCa                                                          <  1:92300/150‑1 (MQ=255)
                                                                                               gagagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg                                                      >  1:1905870/1‑150 (MQ=255)
                                                                                                       cGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaaa                                              >  1:1730329/1‑150 (MQ=255)
                                                                                                       cGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaaa                                              >  1:887495/1‑150 (MQ=255)
                                                                                                       cGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaaa                                              >  2:712288/1‑150 (MQ=255)
                                                                                                          ttGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAg                                           >  1:877357/1‑150 (MQ=255)
                                                                                                          ttGACGGACATTCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAg                                           <  2:761041/150‑1 (MQ=255)
                                                                                                            gCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCa                                         <  2:1732394/150‑1 (MQ=255)
                                                                                                             ccGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCAt                                        <  2:1955084/150‑1 (MQ=255)
                                                                                                               ggACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCt                                      <  2:226032/150‑1 (MQ=255)
                                                                                                                  cATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTc                                   <  1:1969316/150‑1 (MQ=255)
                                                                                                                    tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCg                                                                                               <  2:653363/88‑1 (MQ=255)
                                                                                                                    tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCg                                                                                               >  1:653363/1‑88 (MQ=255)
                                                                                                                     gctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCg                                >  1:1131744/1‑150 (MQ=255)
                                                                                                                      ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg                               >  1:638714/1‑150 (MQ=255)
                                                                                                                       tgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGa                              <  1:1661560/150‑1 (MQ=255)
                                                                                                                           tCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCa                          >  1:136348/1‑150 (MQ=255)
                                                                                                                           tCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCa                          <  1:1012463/150‑1 (MQ=255)
                                                                                                                            ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                         >  2:604478/8‑150 (MQ=39)
                                                                                                                             cACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCt                                      >  2:176711/1‑136 (MQ=255)
                                                                                                                             cACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCt                                      <  1:176711/136‑1 (MQ=255)
                                                                                                                             cACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGc                        <  2:1533458/150‑1 (MQ=255)
                                                                                                                              aCCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCa                       <  1:913776/150‑1 (MQ=255)
                                                                                                                               ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                      >  1:1884668/1‑150 (MQ=255)
                                                                                                                               ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                      >  2:290926/1‑150 (MQ=255)
                                                                                                                               ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                      >  2:1279317/1‑150 (MQ=255)
                                                                                                                               ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACACCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                      <  2:1442588/150‑1 (MQ=255)
                                                                                                                                aagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCa                          <  2:1849131/142‑1 (MQ=255)
                                                                                                                                aagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCa                          >  1:1849131/4‑145 (MQ=255)
                                                                                                                                 tCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCa                    >  2:158987/1‑150 (MQ=255)
                                                                                                                                 agaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCa                    >  1:285761/3‑150 (MQ=255)
                                                                                                                                     tAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGaa                >  1:532484/1‑150 (MQ=255)
                                                                                                                                        gATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGc                                                     <  1:1854293/110‑1 (MQ=255)
                                                                                                                                        gATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGc                                                     >  2:1854293/1‑110 (MQ=255)
                                                                                                                                         aTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCg            >  2:1353717/1‑150 (MQ=255)
                                                                                                                                          tGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGc           <  2:235058/150‑1 (MQ=255)
                                                                                                                                          tGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGc           <  2:1316788/150‑1 (MQ=255)
                                                                                                                                          tGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGc           <  1:1166633/150‑1 (MQ=255)
                                                                                                                                            tATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCg         >  1:739331/1‑150 (MQ=255)
                                                                                                                                               ttATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTc      >  1:524077/1‑150 (MQ=255)
                                                                                                                                               ttATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGAGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTc      <  1:480166/150‑1 (MQ=255)
                                                                                                                                                 atatTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGct   <  1:966658/150‑1 (MQ=255)
                                                                                                                                                   atTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGctt  <  2:524077/150‑1 (MQ=255)
                                                                                                                                                   atTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGctt  <  1:1703865/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGCTT  >  NC_000913/2856690‑2856986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: