BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,908,486	G→C	5.3%	A394G (GCC→GGC)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,908,486	0	G	C	5.3%	327.8 / 6.6	95	A394G (GCC→GGC)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand): ref base G (43/47); new base C (2/3); total (45/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 101 total aligned reads displayed.

GTGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTCC  >  NC_000913/2908337‑2908635

gtgCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTgg                                                                                                                                                       <  2:956441/150‑1 (MQ=255)

gtgCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTgg                                                                                                                                                       <  2:1839565/150‑1 (MQ=255)

gtgCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTgg                                                                                                                                                       <  1:700498/150‑1 (MQ=255)

gtgCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTgg                                                                                                                                                       <  1:707634/150‑1 (MQ=255)

gtgCGCCGAGACGAATGGTACCGCCGACATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTgg                                                                                                                                                       >  2:1621207/1‑150 (MQ=255)

   cgcCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCaa                                                                                                                                                    >  2:949960/1‑150 (MQ=255)

    gcCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAAc                                                                                                                                                   <  1:1024980/150‑1 (MQ=255)

     ccGAGACGCATGGTTCCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACa                                                                                                                                                  >  1:103877/1‑150 (MQ=255)

     ccGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACa                                                                                                                                                  >  2:972511/1‑150 (MQ=255)

         gaCGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGc                                                                                                                                              >  1:1167788/1‑150 (MQ=255)

         gaCGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGc                                                                                                                                              <  1:1540296/150‑1 (MQ=255)

          aCGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCg                                                                                                                                             <  2:26955/150‑1 (MQ=255)

             cATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGCCAACATGgagaac                                                                                                                                          <  1:1589337/150‑3 (MQ=255)

              aTGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGGAACATTGCGAGCg                                                                                                                                         <  2:710199/150‑1 (MQ=255)

               tGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGCCAACATGGAGAACgc                                                                                                                                        <  1:1391852/150‑2 (MQ=255)

                ggTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTa                                                                                                                                       <  1:1598271/150‑1 (MQ=255)

                ggTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTa                                                                                                                                       >  2:1963551/1‑150 (MQ=255)

                ggTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTa                                                                                                                                       >  2:534950/1‑150 (MQ=255)

                 gTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTaa                                                                                                                                      >  1:959092/1‑150 (MQ=255)

                  tACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAAt                                                                                                                                     >  2:1978558/1‑150 (MQ=255)

                  tACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCAAAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAAt                                                                                                                                     >  2:1697221/1‑150 (MQ=255)

                   aCCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATc                                                                                                                                    <  1:1498125/150‑1 (MQ=255)

                    ccgccgAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCGCCATGTTGCCAACATGGAGAACgccaact                                                                                                                                   <  1:965483/150‑7 (MQ=255)

                     cgccgAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCaa                                                                                                                                  >  1:1861810/1‑150 (MQ=255)

                      gccgAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAAt                                                                                                                                 >  2:72728/1‑150 (MQ=255)

                          agaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTaat                                                                                                                             <  1:972511/150‑2 (MQ=255)

                           gaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACg                                                                                                                            >  1:1265216/1‑150 (MQ=255)

                           gaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACg                                                                                                                            <  1:972477/150‑1 (MQ=255)

                           gaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACg                                                                                                                            <  1:578428/150‑1 (MQ=255)

                           gaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACg                                                                                                                            <  1:788273/150‑1 (MQ=255)

                           gaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACg                                                                                                                            >  2:1462902/1‑150 (MQ=255)

                            aTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGc                                                                                                                           <  1:922376/150‑1 (MQ=255)

                             tCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGcc                                                                                                                          >  1:1244215/1‑150 (MQ=255)

                                ctctTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCAcc                                                                                                                       >  1:658046/1‑150 (MQ=255)

                                   ttctCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGc                                                                                                                    <  2:132532/150‑1 (MQ=255)

                                     ctcGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACACCCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCAt                                                                                                                  <  1:1634643/150‑1 (MQ=255)

                                      tcGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATa                                                                                                                 <  1:842533/150‑1 (MQ=255)

                                      tcGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCAAAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATa                                                                                                                 <  1:1697221/150‑1 (MQ=255)

                                          tACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATg                                                                                                                                                >  2:62858/1‑115 (MQ=255)

                                          tACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATg                                                                                                                                                <  1:62858/115‑1 (MQ=255)

                                           aCGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAg                                                                                                            <  1:460554/150‑1 (MQ=255)

                                            cGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAgg                                                                                                           <  1:882034/150‑1 (MQ=255)

                                             gAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGTTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGc                                                                                                          <  2:1167788/150‑1 (MQ=255)

                                                cTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCaaa                                                                                                       >  1:1759002/1‑150 (MQ=255)

                                                  tCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATg                                                                                                     <  1:1462902/150‑1 (MQ=255)

                                                   cAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGc                                                                                                    <  1:1004363/150‑1 (MQ=255)

                                                    aaCGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGcc                                                                                                   >  2:1988742/1‑150 (MQ=255)

                                                    aaCGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGcc                                                                                                   <  1:1834453/150‑1 (MQ=255)

                                                    aaCGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGcc                                                                                                   >  2:269608/1‑150 (MQ=255)

                                                     aCGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGccc                                                                                                  >  1:792205/1‑150 (MQ=255)

                                                      cGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCa                                                                                                 <  2:525331/150‑1 (MQ=255)

                                                          gCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATa                                                                                             <  2:1501224/150‑1 (MQ=255)

                                                                   aTCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATAtt                                                                                    >  2:90447/1‑150 (MQ=255)

                                                                    tCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTg                                                                                   <  2:826489/150‑1 (MQ=255)

                                                                    tCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTg                                                                                   <  2:528178/150‑1 (MQ=255)

                                                                     cgcgCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAAAATTGt                                                                                  >  2:1530088/1‑150 (MQ=255)

                                                                      gcgcCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGtt                                                                                 >  2:1836753/1‑150 (MQ=255)

                                                                       cgcCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCTAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTtc                                                                                <  2:675430/150‑1 (MQ=255)

                                                                        gcCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTtct                                                                               >  2:425626/1‑150 (MQ=255)

                                                                            cTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCAcg                                                                           <  1:1621207/150‑1 (MQ=255)

                                                                             tCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCAcgc                                                                          <  1:1700206/150‑1 (MQ=255)

                                                                               ggTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACgcgc                                                                        <  2:959092/150‑1 (MQ=255)

                                                                                gTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCa                                                                       <  1:949960/150‑1 (MQ=255)

                                                                                  aaTCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCaaa                                                                     <  1:1836753/150‑1 (MQ=255)

                                                                                     cAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcg                                                                  >  2:1840929/1‑150 (MQ=255)

                                                                                     cAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcg                                                                  <  2:741190/150‑1 (MQ=255)

                                                                                      aGCGCCACCACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgc                                                                 >  2:1511607/1‑150 (MQ=255)

                                                                                       gcgcCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgcc                                                                >  1:867348/1‑149 (MQ=255)

                                                                                        cgcCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgcgc                                                               <  2:1861810/150‑1 (MQ=255)

                                                                                         gcaacaACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgcgcg                                                              >  2:1397308/4‑150 (MQ=255)

                                                                                         gcCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAATTGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgcgcg                                                              <  1:1833272/150‑1 (MQ=255)

                                                                                         gcCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgcgcg                                                              <  2:792205/150‑1 (MQ=255)

                                                                                                 cGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAAt                                                      <  1:269608/150‑1 (MQ=255)

                                                                                                   ggTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCa                                                    <  1:72728/150‑1 (MQ=255)

                                                                                                      aCTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGc                                                 >  1:1958272/1‑150 (MQ=255)

                                                                                                               cTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGc                                        <  2:658046/150‑1 (MQ=255)

                                                                                                               cTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGc                                        <  1:1963551/150‑1 (MQ=255)

                                                                                                                 ggCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCa                                      >  2:1816451/1‑150 (MQ=255)

                                                                                                                 ggCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCa                                      >  1:233951/1‑150 (MQ=255)

                                                                                                                   cacaAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACg                                    >  1:341431/1‑150 (MQ=255)

                                                                                                                   cacaAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACg                                    >  1:538281/1‑150 (MQ=255)

                                                                                                                    acaAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGa                                   >  2:974766/1‑150 (MQ=255)

                                                                                                                    acaAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGa                                   >  2:324998/1‑150 (MQ=255)

                                                                                                                     caAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATc                                                     >  1:1015947/1‑131 (MQ=255)

                                                                                                                     caAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATc                                                     <  2:1015947/131‑1 (MQ=255)

                                                                                                                        aTTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGc                               >  1:1046538/1‑150 (MQ=255)

                                                                                                                         ttCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGcc                              <  1:1978558/150‑1 (MQ=255)

                                                                                                                            cGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGaa                           >  1:318890/1‑150 (MQ=255)

                                                                                                                                gagTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGTCTTCTACGCCACGATAGCCGAAACCg                       <  1:780099/150‑1 (MQ=255)

                                                                                                                                gagTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCg                       >  2:1781093/1‑150 (MQ=255)

                                                                                                                                    tGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAg                   >  2:511917/1‑150 (MQ=255)

                                                                                                                                      gCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGt                 >  1:793758/1‑150 (MQ=255)

                                                                                                                                        gctcgccATGTTGCCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTAc               >  1:1428106/6‑150 (MQ=255)

                                                                                                                                        gTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTAc               <  2:192614/150‑1 (MQ=255)

                                                                                                                                        gTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTAc               >  2:1116401/1‑150 (MQ=255)

                                                                                                                                         ctcgccATGTTGCCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACg              >  1:1450880/5‑150 (MQ=255)

                                                                                                                                            tcCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAgg           <  2:341431/150‑1 (MQ=255)

                                                                                                                                             ccATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGa          >  2:1605982/1‑150 (MQ=255)

                                                                                                                                              cATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGTCAAATGCCCAGATAAGGAATATTGTGCTCACACGCAAAACGCGCGGTCGTAATCATGCGTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGAt         <  2:1754801/150‑1 (MQ=255)

                                                                                                                                                     gCAACATGGCGAGCGTAATCAATTAACGCCACCGGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTcc  >  2:1013550/1‑150 (MQ=255)

GTGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTCC  >  NC_000913/2908337‑2908635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`