Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,981,916 T→G 5.4% K89N (AAA→AAC)  araE ← arabinose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,981,9160TG 5.4% 229.7 / 2.6 71K89N (AAA→AAC) araEarabinose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base G (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGAT  >  NC_000913/2981767‑2982065
                                                                                                                                                     |                                                                                                                                                     
tCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAACCAGGATGGCCCCCGCCATCAGGCTGTAt                                                                                                                                                       >  1:825921/1‑150 (MQ=255)
 cAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTAtt                                                                                                                                                      <  2:595445/150‑1 (MQ=255)
 cAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGCCTGTAtt                                                                                                                                                      >  2:287255/1‑150 (MQ=255)
   gAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTa                                                                                                                                                    <  2:831695/150‑1 (MQ=255)
    aaaGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTAc                                                                                                                                                   >  2:725324/1‑150 (MQ=255)
          aCAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCa                                                                                                                                             <  2:1356229/150‑1 (MQ=255)
             gaggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACg                                                                                                                                                  >  2:184165/1‑142 (MQ=255)
             gaggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACg                                                                                                                                                  <  1:184165/142‑1 (MQ=255)
             gaggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGc                                                                                                                                          >  1:967984/1‑150 (MQ=255)
              aggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCg                                                                                                                                         <  1:365964/150‑1 (MQ=255)
              aggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCg                                                                                                                                         <  2:1214434/150‑1 (MQ=255)
                   cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACCTCTCTCCGCTGGTCGCAAACGCGGACCCTATAGAACCGAGTACCAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACg                                                                                                                                    <  1:976747/150‑1 (MQ=255)
                   cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACg                                                                                                                                    <  1:1721861/150‑1 (MQ=255)
                      tgtAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                                                                 <  1:338068/150‑1 (MQ=255)
                      tgtAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                                                                 <  1:345417/150‑1 (MQ=255)
                       gtAAGACGCGATCCCGACAGCACGGCCCAGCACCCCACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                                                                <  2:1312442/150‑1 (MQ=255)
                       gtAAGACGCGATCCCGACAGCAATTCCCAGCACCACACGAGCGGCGATTTACATCTCTACGCGGGGCGCAATAGCGGACCCTATAGAACCGAGTACAAACAGGATGTGCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                                                                <  1:1788143/150‑1 (MQ=255)
                        tAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGc                                                                                                                               >  1:1919500/1‑150 (MQ=255)
                         aaGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                                                              >  1:463982/1‑150 (MQ=255)
                               cgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCAt                                                                                                                        <  2:967984/150‑1 (MQ=255)
                                gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                            >  1:887628/1‑143 (MQ=38)
                                gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                            <  2:887628/145‑3 (MQ=38)
                                 aTCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCCACCATTa                                                                                                                      >  2:1637665/1‑150 (MQ=255)
                                   cccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                                                    >  2:975713/1‑150 (MQ=255)
                                   cccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                                                    <  2:667927/150‑1 (MQ=255)
                                    ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAAc                                                                                                                   >  1:1263934/1‑150 (MQ=255)
                                          gCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCa                                                                                                             >  2:92618/1‑150 (MQ=255)
                                               gCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                                                        >  2:38535/1‑150 (MQ=255)
                                               gCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                                                        <  1:570368/150‑1 (MQ=255)
                                                cccAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAtt                                                                                                       >  1:280180/1‑150 (MQ=255)
                                                  cAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgc                                                                                                     >  2:1369037/1‑150 (MQ=255)
                                                   aGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                                                                    <  2:1263934/150‑1 (MQ=255)
                                                    gCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgctg                                                                                                   >  1:791684/1‑150 (MQ=255)
                                                      accacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAAGCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCa                                                                                                 <  2:1803644/150‑1 (MQ=255)
                                                       ccacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                            <  1:993928/122‑3 (MQ=37)
                                                       ccacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                            >  2:993928/1‑120 (MQ=37)
                                                          cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCACCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGa                                                                                             >  2:698954/1‑150 (MQ=255)
                                                           acGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                                                            <  1:725324/150‑1 (MQ=255)
                                                            cGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGc                                                                                           <  2:791684/150‑1 (MQ=255)
                                                                 ggcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcatcat                                                                                      >  2:1387176/1‑150 (MQ=255)
                                                                   cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCAcc                                                                                               <  1:1765853/139‑1 (MQ=255)
                                                                   cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCAcc                                                                                               >  2:1765853/1‑139 (MQ=255)
                                                                   cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGc                                                                                    >  1:1743051/1‑150 (MQ=255)
                                                                      ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGctac                                                                                 <  1:92618/150‑1 (MQ=255)
                                                                      ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAACATCATGctac                                                                                 <  1:1109061/150‑1 (MQ=255)
                                                                        aaCATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGctacta                                                                               >  2:1800472/1‑150 (MQ=255)
                                                                             ctctACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGAGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccac                                                                          >  2:136326/1‑150 (MQ=255)
                                                                                 aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                                                                         >  1:1281154/1‑147 (MQ=255)
                                                                                 aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                                                                         <  2:1281154/147‑1 (MQ=255)
                                                                                 aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCAt                                                                      <  1:1800472/150‑1 (MQ=255)
                                                                                         cGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCaa                                                              >  2:1073910/1‑150 (MQ=255)
                                                                                           cAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAAc                                                            >  1:1013046/1‑150 (MQ=255)
                                                                                           cAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAAc                                                            >  2:911021/1‑150 (MQ=255)
                                                                                                     cccTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAg                                                  <  1:1369037/150‑1 (MQ=255)
                                                                                                     cccTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCAATTCCTGCAAACGACTGGTCAg                                                  >  1:406645/1‑150 (MQ=255)
                                                                                                       cTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca                                                >  2:458864/1‑150 (MQ=255)
                                                                                                          taGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACaa                                             >  2:225097/1‑150 (MQ=255)
                                                                                                            gAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAg                                           >  2:290161/1‑150 (MQ=255)
                                                                                                            gAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGACCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAg                                           >  2:1286801/1‑150 (MQ=255)
                                                                                                                   gTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt                                       <  1:1870135/147‑1 (MQ=255)
                                                                                                                   gTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt                                       >  2:1870135/1‑147 (MQ=255)
                                                                                                                     aCAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTa                                  <  1:1430438/150‑1 (MQ=255)
                                                                                                                        aaCAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATg                               >  1:1319423/1‑150 (MQ=255)
                                                                                                                        aaCAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGATACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATg                               >  2:223927/1‑150 (MQ=255)
                                                                                                                           aGGATGGCCCCCGCCATCAGGCTTTATTTACGGCACATGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAAc                            <  1:975713/150‑1 (MQ=255)
                                                                                                                            ggATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTACCCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACg                           >  2:1822725/1‑150 (MQ=255)
                                                                                                                            ggATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACg                           <  1:911021/150‑1 (MQ=255)
                                                                                                                                   ccccGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGc                    >  1:1771226/1‑150 (MQ=255)
                                                                                                                                      cGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTcc                 <  2:1848129/150‑1 (MQ=255)
                                                                                                                                           tCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGa            <  2:413254/150‑1 (MQ=255)
                                                                                                                                                 tGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGc      >  1:696453/1‑150 (MQ=255)
                                                                                                                                                  gTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCAATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGcc     <  2:406645/150‑1 (MQ=255)
                                                                                                                                                   tATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCGTCATGCTACTAACCACGCATTTCTGCAAAGGACTGGTCATCAGAAAGTGATAGGTAATGAACGGCAACGCTCCGGCGATTaggttg    <  2:86283/150‑6 (MQ=255)
                                                                                                                                                   tATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCg    <  2:463982/150‑1 (MQ=255)
                                                                                                                                                     tttACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGat  <  2:476437/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGAT  >  NC_000913/2981767‑2982065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑