Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,981,925 C→G 5.6% L86L (CTG→CTC)  araE ← arabinose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,981,9250CG 5.6% 243.3 / 4.8 72L86L (CTG→CTC) araEarabinose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (33/35);  new base G (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

ACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGAC  >  NC_000913/2981777‑2982073
                                                                                                                                                    |                                                                                                                                                    
aCAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCa                                                                                                                                                     <  2:1356229/150‑1 (MQ=255)
   gaggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGc                                                                                                                                                  >  1:967984/1‑150 (MQ=255)
    aggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCg                                                                                                                                                 <  1:365964/150‑1 (MQ=255)
    aggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCg                                                                                                                                                 <  2:1214434/150‑1 (MQ=255)
         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACCTCTCTCCGCTGGTCGCAAACGCGGACCCTATAGAACCGAGTACCAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACg                                                                                                                                            <  1:976747/150‑1 (MQ=255)
         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACg                                                                                                                                            <  1:1721861/150‑1 (MQ=255)
            tgtAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                                                                         <  1:345417/150‑1 (MQ=255)
            tgtAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                                                                         <  1:338068/150‑1 (MQ=255)
             gtAAGACGCGATCCCGACAGCACGGCCCAGCACCCCACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                                                                        <  2:1312442/150‑1 (MQ=255)
             gtAAGACGCGATCCCGACAGCAATTCCCAGCACCACACGAGCGGCGATTTACATCTCTACGCGGGGCGCAATAGCGGACCCTATAGAACCGAGTACAAACAGGATGTGCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                                                                        <  1:1788143/150‑1 (MQ=255)
              tAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGc                                                                                                                                       >  1:1919500/1‑150 (MQ=255)
               aaGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                                                                      >  1:463982/1‑150 (MQ=255)
                     cgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCAt                                                                                                                                <  2:967984/150‑1 (MQ=255)
                      gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                    >  1:887628/1‑143 (MQ=38)
                      gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                    <  2:887628/145‑3 (MQ=38)
                       aTCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCCACCATTa                                                                                                                              >  2:1637665/1‑150 (MQ=255)
                         cccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                                                            <  2:667927/150‑1 (MQ=255)
                         cccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                                                            >  2:975713/1‑150 (MQ=255)
                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAAc                                                                                                                           >  1:1263934/1‑150 (MQ=255)
                                gCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCa                                                                                                                     >  2:92618/1‑150 (MQ=255)
                                     gCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                                                                >  2:38535/1‑150 (MQ=255)
                                     gCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                                                                <  1:570368/150‑1 (MQ=255)
                                      cccAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAtt                                                                                                               >  1:280180/1‑150 (MQ=255)
                                        cAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgc                                                                                                             >  2:1369037/1‑150 (MQ=255)
                                         aGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                                                                            <  2:1263934/150‑1 (MQ=255)
                                          gCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgctg                                                                                                           >  1:791684/1‑150 (MQ=255)
                                            accacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAAGCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCa                                                                                                         <  2:1803644/150‑1 (MQ=255)
                                             ccacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                    >  2:993928/1‑120 (MQ=37)
                                             ccacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                    <  1:993928/122‑3 (MQ=37)
                                                cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCACCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGa                                                                                                     >  2:698954/1‑150 (MQ=255)
                                                 acGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                                                                    <  1:725324/150‑1 (MQ=255)
                                                  cGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGc                                                                                                   <  2:791684/150‑1 (MQ=255)
                                                       ggcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcatcat                                                                                              >  2:1387176/1‑150 (MQ=255)
                                                         cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCAcc                                                                                                       >  2:1765853/1‑139 (MQ=255)
                                                         cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCAcc                                                                                                       <  1:1765853/139‑1 (MQ=255)
                                                         cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGc                                                                                            >  1:1743051/1‑150 (MQ=255)
                                                            ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGctac                                                                                         <  1:92618/150‑1 (MQ=255)
                                                            ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAACATCATGctac                                                                                         <  1:1109061/150‑1 (MQ=255)
                                                              aaCATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGctacta                                                                                       >  2:1800472/1‑150 (MQ=255)
                                                                   ctctACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGAGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccac                                                                                  >  2:136326/1‑150 (MQ=255)
                                                                       aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                                                                                 <  2:1281154/147‑1 (MQ=255)
                                                                       aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                                                                                 >  1:1281154/1‑147 (MQ=255)
                                                                       aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCAt                                                                              <  1:1800472/150‑1 (MQ=255)
                                                                               cGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCaa                                                                      >  2:1073910/1‑150 (MQ=255)
                                                                                 cAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAAc                                                                    >  1:1013046/1‑150 (MQ=255)
                                                                                 cAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAAc                                                                    >  2:911021/1‑150 (MQ=255)
                                                                                           cccTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAg                                                          <  1:1369037/150‑1 (MQ=255)
                                                                                           cccTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCAATTCCTGCAAACGACTGGTCAg                                                          >  1:406645/1‑150 (MQ=255)
                                                                                             cTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca                                                        >  2:458864/1‑150 (MQ=255)
                                                                                                taGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACaa                                                     >  2:225097/1‑150 (MQ=255)
                                                                                                  gAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAg                                                   >  2:290161/1‑150 (MQ=255)
                                                                                                  gAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGACCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAg                                                   >  2:1286801/1‑150 (MQ=255)
                                                                                                         gTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt                                               <  1:1870135/147‑1 (MQ=255)
                                                                                                         gTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt                                               >  2:1870135/1‑147 (MQ=255)
                                                                                                           aCAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTa                                          <  1:1430438/150‑1 (MQ=255)
                                                                                                              aaCAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATg                                       >  1:1319423/1‑150 (MQ=255)
                                                                                                              aaCAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGATACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATg                                       >  2:223927/1‑150 (MQ=255)
                                                                                                                 aGGATGGCCCCCGCCATCAGGCTTTATTTACGGCACATGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAAc                                    <  1:975713/150‑1 (MQ=255)
                                                                                                                  ggATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTACCCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACg                                   >  2:1822725/1‑150 (MQ=255)
                                                                                                                  ggATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACg                                   <  1:911021/150‑1 (MQ=255)
                                                                                                                         ccccGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGc                            >  1:1771226/1‑150 (MQ=255)
                                                                                                                            cGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTcc                         <  2:1848129/150‑1 (MQ=255)
                                                                                                                                 tCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGa                    <  2:413254/150‑1 (MQ=255)
                                                                                                                                       tGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGc              >  1:696453/1‑150 (MQ=255)
                                                                                                                                        gTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCAATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGcc             <  2:406645/150‑1 (MQ=255)
                                                                                                                                         tATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCGTCATGCTACTAACCACGCATTTCTGCAAAGGACTGGTCATCAGAAAGTGATAGGTAATGAACGGCAACGCTCCGGCGATTaggttg            <  2:86283/150‑6 (MQ=255)
                                                                                                                                         tATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCg            <  2:463982/150‑1 (MQ=255)
                                                                                                                                           tttACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGat          <  2:476437/150‑1 (MQ=255)
                                                                                                                                            ttACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGata         <  2:1919500/150‑1 (MQ=255)
                                                                                                                                              aCGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATc       <  1:878696/150‑1 (MQ=255)
                                                                                                                                               cGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCa      <  1:1763251/150‑1 (MQ=255)
                                                                                                                                               cGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCa      >  1:1022636/1‑150 (MQ=255)
                                                                                                                                                   ccAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGAc  >  1:541722/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGAC  >  NC_000913/2981777‑2982073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑