Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,981,931 G→T 5.5% F84L (TTC→TTA)  araE ← arabinose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,981,9310GT 5.5% 244.8 / 3.2 72F84L (TTC→TTA) araEarabinose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (34/34);  new base T (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

CGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATA  >  NC_000913/2981786‑2982079
                                                                                                                                                 |                                                                                                                                                    
cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACCTCTCTCCGCTGGTCGCAAACGCGGACCCTATAGAACCGAGTACCAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACg                                                                                                                                                  <  1:976747/150‑1 (MQ=255)
cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACg                                                                                                                                                  <  1:1721861/150‑1 (MQ=255)
   tgtAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                                                                               <  1:345417/150‑1 (MQ=255)
   tgtAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                                                                               <  1:338068/150‑1 (MQ=255)
    gtAAGACGCGATCCCGACAGCACGGCCCAGCACCCCACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                                                                              <  2:1312442/150‑1 (MQ=255)
    gtAAGACGCGATCCCGACAGCAATTCCCAGCACCACACGAGCGGCGATTTACATCTCTACGCGGGGCGCAATAGCGGACCCTATAGAACCGAGTACAAACAGGATGTGCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                                                                              <  1:1788143/150‑1 (MQ=255)
     tAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGc                                                                                                                                             >  1:1919500/1‑150 (MQ=255)
      aaGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                                                                            >  1:463982/1‑150 (MQ=255)
            cgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCAt                                                                                                                                      <  2:967984/150‑1 (MQ=255)
             gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                          >  1:887628/1‑143 (MQ=38)
             gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                          <  2:887628/145‑3 (MQ=38)
              aTCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCCACCATTa                                                                                                                                    >  2:1637665/1‑150 (MQ=255)
                cccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                                                                  >  2:975713/1‑150 (MQ=255)
                cccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                                                                  <  2:667927/150‑1 (MQ=255)
                 ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAAc                                                                                                                                 >  1:1263934/1‑150 (MQ=255)
                       gCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCa                                                                                                                           >  2:92618/1‑150 (MQ=255)
                            gCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                                                                      >  2:38535/1‑150 (MQ=255)
                            gCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                                                                      <  1:570368/150‑1 (MQ=255)
                             cccAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAtt                                                                                                                     >  1:280180/1‑150 (MQ=255)
                               cAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgc                                                                                                                   >  2:1369037/1‑150 (MQ=255)
                                aGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                                                                                  <  2:1263934/150‑1 (MQ=255)
                                 gCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgctg                                                                                                                 >  1:791684/1‑150 (MQ=255)
                                   accacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAAGCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCa                                                                                                               <  2:1803644/150‑1 (MQ=255)
                                    ccacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                          <  1:993928/122‑3 (MQ=37)
                                    ccacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTCGTTCCGCCTGGGGCGTAAATACAGcctg                                                                                                                                          >  2:993928/1‑120 (MQ=37)
                                       cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCACCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGa                                                                                                           >  2:698954/1‑150 (MQ=255)
                                        acGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                                                                          <  1:725324/150‑1 (MQ=255)
                                         cGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGc                                                                                                         <  2:791684/150‑1 (MQ=255)
                                              ggcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcatcat                                                                                                    >  2:1387176/1‑150 (MQ=255)
                                                cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCAcc                                                                                                             <  1:1765853/139‑1 (MQ=255)
                                                cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCAcc                                                                                                             >  2:1765853/1‑139 (MQ=255)
                                                cgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGc                                                                                                  >  1:1743051/1‑150 (MQ=255)
                                                   ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGctac                                                                                               <  1:92618/150‑1 (MQ=255)
                                                   ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAACATCATGctac                                                                                               <  1:1109061/150‑1 (MQ=255)
                                                     aaCATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGctacta                                                                                             >  2:1800472/1‑150 (MQ=255)
                                                          ctctACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGAGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccac                                                                                        >  2:136326/1‑150 (MQ=255)
                                                              aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                                                                                       >  1:1281154/1‑147 (MQ=255)
                                                              aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                                                                                       <  2:1281154/147‑1 (MQ=255)
                                                              aCGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCAt                                                                                    <  1:1800472/150‑1 (MQ=255)
                                                                      cGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCaa                                                                            >  2:1073910/1‑150 (MQ=255)
                                                                        cAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAAc                                                                          >  2:911021/1‑150 (MQ=255)
                                                                        cAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAAc                                                                          >  1:1013046/1‑150 (MQ=255)
                                                                                  cccTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAg                                                                <  1:1369037/150‑1 (MQ=255)
                                                                                  cccTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCAATTCCTGCAAACGACTGGTCAg                                                                >  1:406645/1‑150 (MQ=255)
                                                                                    cTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca                                                              >  2:458864/1‑150 (MQ=255)
                                                                                       taGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACaa                                                           >  2:225097/1‑150 (MQ=255)
                                                                                         gAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAg                                                         >  2:290161/1‑150 (MQ=255)
                                                                                         gAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGACCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAg                                                         >  2:1286801/1‑150 (MQ=255)
                                                                                                gTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt                                                     <  1:1870135/147‑1 (MQ=255)
                                                                                                gTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt                                                     >  2:1870135/1‑147 (MQ=255)
                                                                                                  aCAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTa                                                <  1:1430438/150‑1 (MQ=255)
                                                                                                     aaCAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATg                                             >  1:1319423/1‑150 (MQ=255)
                                                                                                     aaCAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGATACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATg                                             >  2:223927/1‑150 (MQ=255)
                                                                                                        aGGATGGCCCCCGCCATCAGGCTTTATTTACGGCACATGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAAc                                          <  1:975713/150‑1 (MQ=255)
                                                                                                         ggATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTACCCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACg                                         >  2:1822725/1‑150 (MQ=255)
                                                                                                         ggATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACg                                         <  1:911021/150‑1 (MQ=255)
                                                                                                                ccccGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGc                                  >  1:1771226/1‑150 (MQ=255)
                                                                                                                   cGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTcc                               <  2:1848129/150‑1 (MQ=255)
                                                                                                                        tCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGa                          <  2:413254/150‑1 (MQ=255)
                                                                                                                              tGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGc                    >  1:696453/1‑150 (MQ=255)
                                                                                                                               gTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCAATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGcc                   <  2:406645/150‑1 (MQ=255)
                                                                                                                                tATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCGTCATGCTACTAACCACGCATTTCTGCAAAGGACTGGTCATCAGAAAGTGATAGGTAATGAACGGCAACGCTCCGGCGATTaggttg                  <  2:86283/150‑6 (MQ=255)
                                                                                                                                tATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCg                  <  2:463982/150‑1 (MQ=255)
                                                                                                                                  tttACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGat                <  2:476437/150‑1 (MQ=255)
                                                                                                                                   ttACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGata               <  2:1919500/150‑1 (MQ=255)
                                                                                                                                     aCGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATc             <  1:878696/150‑1 (MQ=255)
                                                                                                                                      cGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCa            <  1:1763251/150‑1 (MQ=255)
                                                                                                                                      cGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCa            >  1:1022636/1‑150 (MQ=255)
                                                                                                                                          ccAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGAc        >  1:541722/1‑150 (MQ=255)
                                                                                                                                               cGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCaaaa   >  1:1065176/1‑149 (MQ=255)
                                                                                                                                               cGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAAt   <  1:698954/150‑1 (MQ=255)
                                                                                                                                               cGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAAt   <  1:592089/150‑1 (MQ=255)
                                                                                                                                                ggAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATa  <  1:306285/150‑1 (MQ=255)
                                                                                                                                                ggAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATa  <  1:1286801/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATA  >  NC_000913/2981786‑2982079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑