Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,067,320 A→T 15.0% intergenic (‑147/+20) ygfI ← / ← yggE putative LysR‑type DNA‑binding transcriptional regulator YgfI/uncharacterized protein YggE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,067,3200AT15.0% 133.5 / 14.0 53intergenic (‑147/+20)ygfI/yggEputative LysR‑type DNA‑binding transcriptional regulator YgfI/uncharacterized protein YggE
Reads supporting (aligned to +/- strand):  ref base A (28/17);  new base T (4/4);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGG  >  NC_000913/3067174‑3067464
                                                                                                                                                  |                                                                                                                                                
ccccctcccccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTAtt                                                                                                                                               <  1:1637063/150‑2 (MQ=255)
        cccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                                                                       <  2:396807/150‑1 (MQ=255)
         ccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCt                                                                                                                                      <  2:1000220/150‑1 (MQ=255)
         ccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCt                                                                                                                                      >  1:1396044/1‑150 (MQ=255)
         ccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAGTAGCCCGACGTATTCGTCGGGCt                                                                                                                                      <  1:1907302/150‑1 (MQ=255)
           gCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTaa                                                                                                                                    <  1:955428/150‑2 (MQ=255)
                       gCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAATATAGGTGATTACGTCATTGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgt                                                                                                                        <  2:1045130/150‑1 (MQ=255)
                         ttAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                                                                      <  1:145541/150‑1 (MQ=255)
                              cGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAg                                                                                                                 >  2:182517/1‑150 (MQ=255)
                               gTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                                                                                                >  1:511189/1‑150 (MQ=255)
                                    agtcagCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGttt                                                                                                           >  1:1831169/1‑150 (MQ=255)
                                         gCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                                                                                                      >  1:1023583/1‑150 (MQ=255)
                                         gCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                                                                                                      >  1:1016737/1‑150 (MQ=255)
                                           cTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCggg                                                                                                    >  2:564925/1‑150 (MQ=255)
                                                 ttGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGttgttg                                                                                              <  1:1526859/150‑1 (MQ=255)
                                                 ttGATGAAACCGCGAGCAAAGATAGGTGATAACGTCATGGTTTTACAGAAAATTACAGAAAAAGGCCGCAATATCGGGTACCGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGttgttg                                                                                              <  2:588065/150‑1 (MQ=255)
                                                   gatgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGAt                                                                                            >  2:434636/1‑150 (MQ=255)
                                                    attaaaCCGCCAGCAAACTAAGGTCATTACGTCATGGTTTAACATAAAATAACCCAAAACGGAGCAAATATCGGGTAACGGCTTACGCCCGACTATTACGTCGGGCTACAAATA‑TATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                                                          <  2:222811/147‑1 (MQ=255)
                                                              gAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCt                                                                                 <  2:1885072/150‑1 (MQ=255)
                                                               aGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAAGATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGGTCTa                                                                                >  2:1728593/1‑150 (MQ=255)
                                                                  aaaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACt                                                                             >  2:671480/1‑150 (MQ=255)
                                                                   aaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTg                                                                            <  1:1810413/150‑1 (MQ=255)
                                                                   aaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTg                                                                            <  1:564925/150‑1 (MQ=255)
                                                                       tAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAg                                                                        <  1:671480/150‑1 (MQ=255)
                                                                       tAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGAAGAAAACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGTAAg                                                                        >  1:1902128/1‑150 (MQ=255)
                                                                        aGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGa                                                                       <  1:182517/150‑1 (MQ=255)
                                                                         ggTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGAc                                                                      >  2:351226/1‑150 (MQ=255)
                                                                         ggTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGAc                                                                      >  1:1299321/1‑150 (MQ=255)
                                                                                              cataaaaatacataaaaATGAGCCAATATCAGGTAAAGGCATTAAACCGAAGAATAAGTCGGTCTACAAATATTATTGAACTAAAAGTGTTTTAGAGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaaa                                                 >  2:1650974/4‑150 (MQ=255)
                                                                                                gaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg                                                          >  1:1388138/1‑139 (MQ=255)
                                                                                                gaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg                                                          <  2:1388138/139‑1 (MQ=255)
                                                                                                gaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACt                                               >  1:936991/1‑150 (MQ=255)
                                                                                                      tacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAg                                         >  2:970723/1‑150 (MQ=255)
                                                                                                        cagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCg                                       >  2:1064592/1‑150 (MQ=255)
                                                                                                         agaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCgg                                      <  2:511189/150‑1 (MQ=255)
                                                                                                          gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGc                                     >  2:1472932/1‑150 (MQ=255)
                                                                                                               aggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCt                                >  1:1185868/1‑150 (MQ=255)
                                                                                                                 gaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCg                              >  1:1762867/1‑150 (MQ=255)
                                                                                                                            gggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTg                   >  1:1459760/1‑150 (MQ=255)
                                                                                                                                aaaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCg               >  1:1399942/1‑150 (MQ=255)
                                                                                                                                  aGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGa             >  1:267510/1‑150 (MQ=255)
                                                                                                                                   ggCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGac            <  2:1396044/150‑1 (MQ=255)
                                                                                                                                   ggCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGac            >  2:626107/1‑150 (MQ=255)
                                                                                                                                    gCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGaca           <  2:1831169/150‑1 (MQ=255)
                                                                                                                                     cATTAGCCCGCCGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGacac          >  1:1882507/1‑150 (MQ=255)
                                                                                                                                     cATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGacac          <  2:1299321/150‑1 (MQ=255)
                                                                                                                                     cATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGacac          >  2:1184661/1‑150 (MQ=255)
                                                                                                                                      aTTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACAcc         <  2:1762867/150‑1 (MQ=255)
                                                                                                                                        tAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCgg       >  1:1337342/1‑150 (MQ=255)
                                                                                                                                         aGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc      >  1:579217/1‑150 (MQ=255)
                                                                                                                                         aGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc      >  1:198775/1‑150 (MQ=255)
                                                                                                                                         aGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc      >  1:191840/1‑150 (MQ=255)
                                                                                                                                         aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc      >  1:964924/1‑150 (MQ=255)
                                                                                                                                             cgacgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGATGTTGAGATCGACCTGATCATCAAACTGTATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACTCCGGCGCgg  <  2:1144811/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGG  >  NC_000913/3067174‑3067464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑