Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,153 A→T 5.3% K497* (AAA→TAA) ‡ fadH → 2,4‑dienoyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,1530AT 5.3% 309.9 / 5.1 94K497* (AAA→TAA) ‡fadH2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base A (46/43);  new base T (2/3);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

ACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAG  >  NC_000913/3233004‑3233298
                                                                                                                                                     |                                                                                                                                                 
acacCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGcaaca                                                                                                                                                   >  1:561398/1‑150 (MQ=255)
 cacCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGcaacaa                                                                                                                                                  >  2:1118017/1‑150 (MQ=255)
 cacCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGcaacaa                                                                                                                                                  >  2:1762558/1‑150 (MQ=255)
   ccGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAg                                                                                                                                                <  1:945834/150‑1 (MQ=255)
   ccGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAg                                                                                                                                                >  1:429363/1‑150 (MQ=255)
      tGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTg                                                                                                                                             >  1:1474927/1‑150 (MQ=255)
       gACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGc                                                                                                                                            >  2:202382/1‑150 (MQ=255)
         cggcggATCAGTTACAGGCTTTCGATGATACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                          <  2:141991/150‑1 (MQ=255)
          ggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCcat                                                                                                                                         <  2:1525550/150‑1 (MQ=255)
           gcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaac                                                                                                                                        <  2:1714849/150‑3 (MQ=255)
           cgagataactcaataccttcggatgatcgatcccgtcgatgggcggagtgcgcggcacgatcccactggcgaggatcgtttcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCcatc                                                                                                                                        >  2:1412730‑M2/86‑150 (MQ=255)
            cggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaacc                                                                                                                                       <  2:242618/150‑4 (MQ=255)
            cggACCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaacc                                                                                                                                       <  2:193378/150‑4 (MQ=255)
             ggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCcatcat                                                                                                                                      >  2:260589/1‑150 (MQ=255)
              gATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCatcatc                                                                                                                                     >  1:1687866/1‑150 (MQ=255)
                tCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCgg                                                                                                                                   <  1:1175797/150‑1 (MQ=255)
                tCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCgg                                                                                                                                   <  2:950653/150‑1 (MQ=255)
                  aGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGtt                                                                                                                                 >  2:592957/1‑150 (MQ=255)
                   gTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTtg                                                                                                                                >  1:1510441/1‑150 (MQ=255)
                        agtcTTTCTATGAAACGATCCTCGCCAGTGGTATCGTGCTGCGCACTCCGCCCATCGACGGTATCGATCATCCGAATGTATTGAGTTATCTCGATTTACTGCGCGACAAAGCGCCTGTTGGCAACAAAGTTGCCATCATCGTTTGTGGCg                                                                                                                           <  2:851206/147‑1 (MQ=255)
                        aGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCg                                                                                                                           >  1:276912/1‑150 (MQ=255)
                          gCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                         <  1:379087/150‑1 (MQ=255)
                          gCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCAGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                         <  1:1302099/150‑1 (MQ=255)
                             ttCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAtt                                                                                                                      >  1:784651/1‑150 (MQ=255)
                              tCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTCCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTg                                                                                                                     <  2:1197122/150‑1 (MQ=255)
                              cggatgatcgatcccgtcgatgggcggagtgcgcggcacgatcccactggcgaggatcgtttcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAGTg                                                                                                                     >  2:574796‑M2/67‑150 (MQ=255)
                               ggatgatcgatcccgtcgatgggcggagtgcgcggcacgatcccactggtgaggatcgtttcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTgg                                                                                                                    >  1:733422‑M2/66‑150 (MQ=255)
                               cGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTgg                                                                                                                    >  1:1070051/1‑150 (MQ=255)
                                gatgatcgatcccgtcgatgggcggagtgcgcggcacgatcccactggcgaggatcgtttcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGt                                                                                                                   >  1:1216639‑M2/65‑150 (MQ=255)
                                   GAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCAATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCACCAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtttt                                                                                                                <  1:888059‑M1/150‑1 (MQ=255)
                                    AAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                               <  1:1908201‑M1/150‑1 (MQ=255)
                                     atcgatcccgtcgatgggcggagtgcgcggcacgatcccactggcgaggatcgatcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGa                                                                                                              >  1:75347‑M2/60‑150 (MQ=255)
                                     aaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGa                                                                                                              <  1:914510/150‑1 (MQ=255)
                                       cGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATa                                                                                                            >  1:1852363/1‑150 (MQ=255)
                                         aTCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACg                                                                                                          >  2:1357748/1‑150 (MQ=255)
                                          tCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACgg                                                                                                         <  2:1510441/150‑1 (MQ=255)
                                          tCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACgg                                                                                                         <  2:648946/150‑1 (MQ=255)
                                           ccTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGc                                                                                                        >  1:560915/1‑150 (MQ=255)
                                            cTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCg                                                                                                       >  2:838621/1‑150 (MQ=255)
                                             tCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGa                                                                                                      <  1:19180/150‑1 (MQ=255)
                                               gCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATg                                                                                                    <  1:472758/150‑1 (MQ=255)
                                                 cAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTa                                                                                                  <  2:1844113/150‑1 (MQ=255)
                                                 cAGTGGGATCGTGCCGCGCACTCCGCCCATCGACAGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTa                                                                                                  <  2:906400/150‑1 (MQ=255)
                                                  aGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAt                                                                                                 >  1:1188730/1‑150 (MQ=255)
                                                  aGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAt                                                                                                 >  1:410162/1‑150 (MQ=255)
                                                    tGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAttt                                                                                               <  1:1762558/150‑1 (MQ=255)
                                                     gggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTa                                                                                              >  1:1728914/1‑150 (MQ=255)
                                                       gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                            <  1:838621/150‑1 (MQ=255)
                                                          cGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCa                                                                                         >  2:82618/1‑150 (MQ=255)
                                                           gTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGGTGGGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAg                                                                                        <  2:185657/150‑1 (MQ=255)
                                                             gCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGcc                                                                                      >  2:1734918/1‑150 (MQ=255)
                                                             gCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGcc                                                                                      <  2:429363/150‑1 (MQ=255)
                                                             gCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGcc                                                                                      >  1:1238515/1‑150 (MQ=255)
                                                                gcgcACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCggg                                                                                   >  1:662231/1‑150 (MQ=255)
                                                                gcgcACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCggg                                                                                   <  1:1246071/150‑1 (MQ=255)
                                                                     cTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAAt                                                                              <  2:831840/150‑1 (MQ=255)
                                                                     cTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAAt                                                                              >  2:1628673/1‑150 (MQ=255)
                                                                         gCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATccac                                                                          >  2:1560609/1‑150 (MQ=255)
                                                                          cccATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATccacc                                                                         >  2:1414148/1‑150 (MQ=255)
                                                                          cccATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATccacc                                                                         >  2:1635708/1‑150 (MQ=255)
                                                                           ccATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATccacca                                                                        <  1:1027743/150‑1 (MQ=255)
                                                                            cATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccag                                                                       >  1:1227312/1‑150 (MQ=255)
                                                                            cATCGACGGGATCGATCATCCGAAGCTATTCAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCAACCTCGCATGTGGCGCGATAGGGTTTGATACGGCGATGTATTTAAGTCAGACGGACGAATCGAccag                                                                       >  1:861380/1‑150 (MQ=255)
                                                                             aTCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccagc                                                                      <  1:1084890/150‑1 (MQ=255)
                                                                             aTCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccagc                                                                      >  1:3386/1‑150 (MQ=255)
                                                                                   aggATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAat                                                                <  2:75347/149‑1 (MQ=255)
                                                                                         gatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCTCCAGCCAGAATATCGcc                                                          >  2:1279291/1‑150 (MQ=255)
                                                                                           tcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAATTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCgg                                                        <  2:1914084‑M2/145‑1 (MQ=255)
                                                                                           tcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCgg                                                        <  2:784651‑M2/145‑1 (MQ=255)
                                                                                             atcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGt                                                      <  2:1216639‑M2/147‑1 (MQ=255)
                                                                                              tcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGt                                                                                                   >  1:450374‑M2/3‑104 (MQ=255)
                                                                                              tcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGt                                                                                                   <  2:450374‑M2/102‑1 (MQ=255)
                                                                                              tcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGtt                                                     <  1:202382‑M2/148‑1 (MQ=255)
                                                                                              tcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGtt                                                     >  2:1407320‑M2/3‑150 (MQ=255)
                                                                                                cGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCt                                                   >  1:713433/1‑150 (MQ=255)
                                                                                                cGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCt                                                   >  2:1022390/1‑150 (MQ=255)
                                                                                                  aaGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGt                                                 <  1:260589/150‑1 (MQ=255)
                                                                                                    ggTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaa                                               >  1:890506/1‑150 (MQ=255)
                                                                                                     gTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaat                                              <  2:1227312/150‑1 (MQ=255)
                                                                                                      tATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatg                                             <  2:224498/150‑1 (MQ=255)
                                                                                                       aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatga                                            >  1:1598492/1‑150 (MQ=255)
                                                                                                                  tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                 >  1:803440/1‑150 (MQ=255)
                                                                                                                  tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                 >  1:1318380/1‑150 (MQ=255)
                                                                                                                  tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                 <  1:592957/150‑1 (MQ=255)
                                                                                                                  tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                 <  2:3386/150‑1 (MQ=255)
                                                                                                                             cgcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTa                      <  1:1118017/150‑1 (MQ=255)
                                                                                                                              gcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTac                     >  1:1189444/1‑150 (MQ=255)
                                                                                                                                 aCAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGa                                                                  >  1:1796801/1‑102 (MQ=255)
                                                                                                                                 aCAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGa                                                                  <  2:1796801/102‑1 (MQ=255)
                                                                                                                                 aCAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTacaac                  <  1:82618/150‑1 (MQ=255)
                                                                                                                                     aGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGc              <  2:1238515/150‑1 (MQ=255)
                                                                                                                                      gcgcCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCt             <  2:700800/150‑1 (MQ=255)
                                                                                                                                         ccGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggt          <  1:1574469/150‑1 (MQ=255)
                                                                                                                                            gatGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGc       >  1:1903209/3‑150 (MQ=255)
                                                                                                                                             atGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCt      >  2:985466/2‑150 (MQ=255)
                                                                                                                                              tGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCtt     >  2:1588328/1‑150 (MQ=255)
                                                                                                                                                gCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTaa   >  1:1510637/1‑150 (MQ=255)
                                                                                                                                                 caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTac                     >  2:845121/1‑131 (MQ=255)
                                                                                                                                                 caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTac                     <  1:845121/131‑1 (MQ=255)
                                                                                                                                                 caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAg  >  1:1786124/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAG  >  NC_000913/3233004‑3233298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑