Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,445 A→T 5.4% V100V (GTT→GTA) ‡ ygjV ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,4450AT 5.4% 300.3 / 3.4 94V100V (GTT→GTA) ‡ygjVinner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (45/43);  new base T (3/2);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

AGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGAT  >  NC_000913/3241296‑3241593
                                                                                                                                                     |                                                                                                                                                    
aGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATa                                                                                                                                                      >  2:412892/1‑150 (MQ=255)
     aaaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCgg                                                                                                                                                 >  1:1958878/1‑150 (MQ=255)
      aaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGc                                                                                                                                                <  1:845884/150‑1 (MQ=255)
         cTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAAt                                                                                                                                             <  1:412892/150‑1 (MQ=255)
         cTACACTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAAt                                                                                                                                             >  1:726616/1‑150 (MQ=255)
           aCCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGGTTATCGGCacgat                                                                                                                                           <  2:1007118/150‑5 (MQ=255)
           aCCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATGACCGGCAATAg                                                                                                                                           >  1:1906025/1‑150 (MQ=255)
             ccTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGGTTATCGGCacgattg                                                                                                                                         <  1:1516541/150‑7 (MQ=255)
              cTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                        <  2:452814/150‑1 (MQ=255)
              cTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                        <  2:445747/150‑1 (MQ=255)
              cTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                        <  1:1692208/150‑1 (MQ=255)
               tCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCg                                                                                                                                       <  2:252256/150‑1 (MQ=255)
                    catcGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                                  >  2:1987052/1‑150 (MQ=255)
                        gTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGgatga                                                                                                                              <  1:289739/150‑1 (MQ=255)
                            cgccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGa                                                                                                                          >  2:721757/1‑150 (MQ=255)
                            cgccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGa                                                                                                                          <  1:938387/150‑1 (MQ=255)
                            cgccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGa                                                                                                                          <  1:1924379/150‑1 (MQ=255)
                              ccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAAc                                                                                                                        <  2:891423/150‑1 (MQ=255)
                              ccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAAc                                                                                                                        <  2:726616/150‑1 (MQ=255)
                                tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                                  >  1:1406579/1‑138 (MQ=255)
                                tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                                  <  2:1406579/138‑1 (MQ=255)
                                tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACtt                                                                                                                      >  1:319531/1‑150 (MQ=255)
                                    gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCTCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcgcg                                                                                                                  >  2:142046/1‑150 (MQ=255)
                                    gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcgcg                                                                                                                  >  2:1532287/1‑150 (MQ=255)
                                    gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACATTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTGCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                        >  2:1191211/1‑128 (MQ=255)
                                    gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACATTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTGCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                        <  1:1191211/128‑1 (MQ=255)
                                        ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                              >  2:1370891/1‑150 (MQ=255)
                                        ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                              >  1:684808/1‑150 (MQ=255)
                                               gAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTcc                                                                                                       >  2:1802582/1‑150 (MQ=255)
                                                 aGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGc                                                                                                     >  2:108354/1‑150 (MQ=255)
                                                      tgAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTc                                                                                                <  1:1797093/150‑1 (MQ=255)
                                                            aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAgg                                                                                                               <  2:1817923/129‑1 (MQ=255)
                                                            aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAgg                                                                                                               >  1:1817923/1‑129 (MQ=255)
                                                            aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGcaca                                                                                          >  1:1337117/1‑150 (MQ=255)
                                                             cccAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACaa                                                                                         >  1:512242/1‑150 (MQ=255)
                                                             cccAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACaa                                                                                         >  2:623207/1‑150 (MQ=255)
                                                              ccAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAAt                                                                                        <  1:1121278/150‑1 (MQ=255)
                                                                  caacaaGTTGAAAACCACATTAGGCAGGGCATGGTCAGGCCTTTAGAGCAGAATAGCGGCCAGGTACTGACAATCGTGCCGCTAACGGGCAATAGTTCGACAGGATGTTGGATCTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaa                                                                                    <  2:1924876/150‑1 (MQ=255)
                                                                   aaCAGGTTGAAAAGCACTTTACGCAGCGCATTGTCAGCCCTTTACAGCAGAATAGCGCGCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGTAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaaa                                                                                   <  2:323133/150‑1 (MQ=255)
                                                                       cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                               >  2:1355765/1‑150 (MQ=255)
                                                                       cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                               >  2:1863291/1‑150 (MQ=255)
                                                                        gTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGTCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGcc                                                                              <  1:679727/150‑1 (MQ=255)
                                                                        gTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGcc                                                                              >  2:1760294/1‑150 (MQ=255)
                                                                         ttGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGAGCCAAGGTACTTACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTAGCGAGGCCAAATACGCCAGTCAGCACAATAAAAATCGCCa                                                                             >  1:1651598/1‑150 (MQ=255)
                                                                             aaaCCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTAGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAc                                                                         >  2:1094809/1‑150 (MQ=255)
                                                                               aCCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAccc                                                                       >  2:1136279/1‑150 (MQ=255)
                                                                                 cacaTTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCAt                                                                     <  1:1773897/150‑1 (MQ=255)
                                                                                   caTTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATaa                                                                   <  2:1958878/150‑1 (MQ=255)
                                                                                        cGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgc                                                              >  2:627431/1‑150 (MQ=255)
                                                                                        cGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgc                                                              <  1:1802582/150‑1 (MQ=255)
                                                                                          cAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgcgc                                                            >  1:1089242/1‑150 (MQ=255)
                                                                                           aGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGcgcg                                                           <  1:1987052/150‑1 (MQ=255)
                                                                                              gcATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCgcgtgc                                                        >  1:1998363/1‑150 (MQ=255)
                                                                                                   gTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                   >  1:1163697/1‑150 (MQ=255)
                                                                                                   gTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                   >  1:264517/1‑150 (MQ=255)
                                                                                                    tCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAgg                                                  <  1:623207/150‑1 (MQ=255)
                                                                                                    tCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAgg                                                  >  2:1658347/1‑150 (MQ=255)
                                                                                                       gCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                   <  2:1195291/146‑1 (MQ=255)
                                                                                                       gCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                   >  1:1195291/1‑146 (MQ=255)
                                                                                                         ccTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATc                                             >  1:1164030/1‑150 (MQ=255)
                                                                                                          cTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCa                                            <  2:1224782/150‑1 (MQ=255)
                                                                                                          cTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCa                                            >  1:1946655/1‑150 (MQ=255)
                                                                                                           tttACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCaa                                           >  1:952151/1‑150 (MQ=255)
                                                                                                             tACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATg                                         <  1:56322/150‑1 (MQ=255)
                                                                                                             tACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATg                                         <  2:512242/150‑1 (MQ=255)
                                                                                                              aCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGt                                        <  1:1957052/150‑1 (MQ=255)
                                                                                                              aCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGt                                        <  2:319531/150‑1 (MQ=255)
                                                                                                               cagcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTa                                       >  1:724492/1‑150 (MQ=255)
                                                                                                                 gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAAATCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACg                                     >  2:818343/1‑150 (MQ=255)
                                                                                                                    gAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                  >  1:1789750/1‑150 (MQ=255)
                                                                                                                    gAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                  >  1:1144485/1‑150 (MQ=255)
                                                                                                                    gAATACCGCCAAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                  >  2:1888426/1‑150 (MQ=255)
                                                                                                                     aaTAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                 >  1:1969274/1‑150 (MQ=255)
                                                                                                                     aaTAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                 <  1:1863291/150‑1 (MQ=255)
                                                                                                                       tAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGc                               <  1:1534473/150‑1 (MQ=255)
                                                                                                                             ccAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAg                         <  2:1337117/150‑1 (MQ=255)
                                                                                                                                 gTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtg                     <  1:1532287/150‑1 (MQ=255)
                                                                                                                                  tACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtgg                    <  1:142046/150‑1 (MQ=255)
                                                                                                                                     tGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcac                 <  1:1136279/150‑1 (MQ=255)
                                                                                                                                      gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact                <  2:952151/150‑1 (MQ=255)
                                                                                                                                      gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact                >  1:36885/1‑150 (MQ=255)
                                                                                                                                        aactattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactgg              >  2:475946/7‑150 (MQ=255)
                                                                                                                                         actattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc             >  2:1953294/6‑150 (MQ=255)
                                                                                                                                         actattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc             >  1:1613680/6‑150 (MQ=255)
                                                                                                                                         aaTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc             <  2:1789750/150‑1 (MQ=255)
                                                                                                                                         aaTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc             >  2:625700/1‑150 (MQ=255)
                                                                                                                                          aTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggca            <  1:1483690/150‑1 (MQ=255)
                                                                                                                                          aTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggca            >  2:688091/1‑150 (MQ=255)
                                                                                                                                            cGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCAcc          >  2:1730563/1‑150 (MQ=255)
                                                                                                                                            cGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCAcc          <  1:1866859/150‑1 (MQ=255)
                                                                                                                                               gCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                 >  1:1319115/1‑124 (MQ=255)
                                                                                                                                               gCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                 <  2:1319115/124‑1 (MQ=255)
                                                                                                                                                cctaTAACCGGCAATAGTTCGACACGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCg      >  2:614191/4‑150 (MQ=255)
                                                                                                                                                  gATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCggg    <  1:1760294/150‑1 (MQ=255)
                                                                                                                                                    tAAGCGTCAATAGTTCGACAGGATGATGTATCTTCGGGAGGCGAATTCCGCCAGTCAGCACAATAAATATCGCCATTACCCTTAAGCTGCGCTTGCGTAATTTAATCTTTGTAAGAATGGCATTAAGGATGGCACTGGCACCAGCGGGAt  <  2:1651598/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGAT  >  NC_000913/3241296‑3241593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑