Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,447 C→T 5.3% V100I (GTT→ATT) ‡ ygjV ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,4470CT 5.3% 298.0 / 3.9 94V100I (GTT→ATT) ‡ygjVinner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (46/42);  new base T (3/2);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

AAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGG  >  NC_000913/3241301‑3241596
                                                                                                                                                  |                                                                                                                                                     
aaaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCgg                                                                                                                                                    >  1:1958878/1‑150 (MQ=255)
 aaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGc                                                                                                                                                   <  1:845884/150‑1 (MQ=255)
    cTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAAt                                                                                                                                                <  1:412892/150‑1 (MQ=255)
    cTACACTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAAt                                                                                                                                                >  1:726616/1‑150 (MQ=255)
      aCCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGGTTATCGGCacgat                                                                                                                                              <  2:1007118/150‑5 (MQ=255)
      aCCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATGACCGGCAATAg                                                                                                                                              >  1:1906025/1‑150 (MQ=255)
        ccTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGGTTATCGGCacgattg                                                                                                                                            <  1:1516541/150‑7 (MQ=255)
         cTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                           <  1:1692208/150‑1 (MQ=255)
         cTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                           <  2:445747/150‑1 (MQ=255)
         cTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                           <  2:452814/150‑1 (MQ=255)
          tCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCg                                                                                                                                          <  2:252256/150‑1 (MQ=255)
               catcGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                                     >  2:1987052/1‑150 (MQ=255)
                   gTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGgatga                                                                                                                                 <  1:289739/150‑1 (MQ=255)
                       cgccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGa                                                                                                                             <  1:938387/150‑1 (MQ=255)
                       cgccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGa                                                                                                                             <  1:1924379/150‑1 (MQ=255)
                       cgccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGa                                                                                                                             >  2:721757/1‑150 (MQ=255)
                         ccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAAc                                                                                                                           <  2:726616/150‑1 (MQ=255)
                         ccTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAAc                                                                                                                           <  2:891423/150‑1 (MQ=255)
                           tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                                     >  1:1406579/1‑138 (MQ=255)
                           tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                                     <  2:1406579/138‑1 (MQ=255)
                           tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACtt                                                                                                                         >  1:319531/1‑150 (MQ=255)
                               gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCTCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcgcg                                                                                                                     >  2:142046/1‑150 (MQ=255)
                               gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcgcg                                                                                                                     >  2:1532287/1‑150 (MQ=255)
                               gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACATTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTGCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                           <  1:1191211/128‑1 (MQ=255)
                               gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACATTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTGCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTc                                                                                                                                           >  2:1191211/1‑128 (MQ=255)
                                   ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                                 >  1:684808/1‑150 (MQ=255)
                                   ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                                 >  2:1370891/1‑150 (MQ=255)
                                          gAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTcc                                                                                                          >  2:1802582/1‑150 (MQ=255)
                                            aGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGc                                                                                                        >  2:108354/1‑150 (MQ=255)
                                                 tgAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTc                                                                                                   <  1:1797093/150‑1 (MQ=255)
                                                       aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAgg                                                                                                                  <  2:1817923/129‑1 (MQ=255)
                                                       aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAgg                                                                                                                  >  1:1817923/1‑129 (MQ=255)
                                                       aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGcaca                                                                                             >  1:1337117/1‑150 (MQ=255)
                                                        cccAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACaa                                                                                            >  1:512242/1‑150 (MQ=255)
                                                        cccAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACaa                                                                                            >  2:623207/1‑150 (MQ=255)
                                                         ccAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAAt                                                                                           <  1:1121278/150‑1 (MQ=255)
                                                             caacaaGTTGAAAACCACATTAGGCAGGGCATGGTCAGGCCTTTAGAGCAGAATAGCGGCCAGGTACTGACAATCGTGCCGCTAACGGGCAATAGTTCGACAGGATGTTGGATCTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaa                                                                                       <  2:1924876/150‑1 (MQ=255)
                                                              aaCAGGTTGAAAAGCACTTTACGCAGCGCATTGTCAGCCCTTTACAGCAGAATAGCGCGCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGTAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaaa                                                                                      <  2:323133/150‑1 (MQ=255)
                                                                  cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                                  >  2:1355765/1‑150 (MQ=255)
                                                                  cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                                  >  2:1863291/1‑150 (MQ=255)
                                                                   gTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGTCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGcc                                                                                 <  1:679727/150‑1 (MQ=255)
                                                                   gTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGcc                                                                                 >  2:1760294/1‑150 (MQ=255)
                                                                    ttGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGAGCCAAGGTACTTACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTAGCGAGGCCAAATACGCCAGTCAGCACAATAAAAATCGCCa                                                                                >  1:1651598/1‑150 (MQ=255)
                                                                        aaaCCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTAGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAc                                                                            >  2:1094809/1‑150 (MQ=255)
                                                                          aCCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAccc                                                                          >  2:1136279/1‑150 (MQ=255)
                                                                            cacaTTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCAt                                                                        <  1:1773897/150‑1 (MQ=255)
                                                                              caTTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATaa                                                                      <  2:1958878/150‑1 (MQ=255)
                                                                                   cGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgc                                                                 >  2:627431/1‑150 (MQ=255)
                                                                                   cGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgc                                                                 <  1:1802582/150‑1 (MQ=255)
                                                                                     cAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgcgc                                                               >  1:1089242/1‑150 (MQ=255)
                                                                                      aGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGcgcg                                                              <  1:1987052/150‑1 (MQ=255)
                                                                                         gcATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCgcgtgc                                                           >  1:1998363/1‑150 (MQ=255)
                                                                                              gTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                      >  1:264517/1‑150 (MQ=255)
                                                                                              gTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                      >  1:1163697/1‑150 (MQ=255)
                                                                                               tCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAgg                                                     <  1:623207/150‑1 (MQ=255)
                                                                                               tCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAgg                                                     >  2:1658347/1‑150 (MQ=255)
                                                                                                  gCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                      <  2:1195291/146‑1 (MQ=255)
                                                                                                  gCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAg                                                      >  1:1195291/1‑146 (MQ=255)
                                                                                                    ccTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATc                                                >  1:1164030/1‑150 (MQ=255)
                                                                                                     cTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCa                                               >  1:1946655/1‑150 (MQ=255)
                                                                                                     cTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCa                                               <  2:1224782/150‑1 (MQ=255)
                                                                                                      tttACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCaa                                              >  1:952151/1‑150 (MQ=255)
                                                                                                        tACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATg                                            <  2:512242/150‑1 (MQ=255)
                                                                                                        tACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATg                                            <  1:56322/150‑1 (MQ=255)
                                                                                                         aCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGt                                           <  2:319531/150‑1 (MQ=255)
                                                                                                         aCAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGt                                           <  1:1957052/150‑1 (MQ=255)
                                                                                                          cagcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTa                                          >  1:724492/1‑150 (MQ=255)
                                                                                                            gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAAATCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACg                                        >  2:818343/1‑150 (MQ=255)
                                                                                                               gAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                     >  1:1144485/1‑150 (MQ=255)
                                                                                                               gAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                     >  1:1789750/1‑150 (MQ=255)
                                                                                                               gAATACCGCCAAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                     >  2:1888426/1‑150 (MQ=255)
                                                                                                                aaTAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                    >  1:1969274/1‑150 (MQ=255)
                                                                                                                aaTAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                    <  1:1863291/150‑1 (MQ=255)
                                                                                                                  tAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGc                                  <  1:1534473/150‑1 (MQ=255)
                                                                                                                        ccAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAg                            <  2:1337117/150‑1 (MQ=255)
                                                                                                                            gTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtg                        <  1:1532287/150‑1 (MQ=255)
                                                                                                                             tACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtgg                       <  1:142046/150‑1 (MQ=255)
                                                                                                                                tGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcac                    <  1:1136279/150‑1 (MQ=255)
                                                                                                                                 gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact                   <  2:952151/150‑1 (MQ=255)
                                                                                                                                 gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact                   >  1:36885/1‑150 (MQ=255)
                                                                                                                                   aactattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactgg                 >  2:475946/7‑150 (MQ=255)
                                                                                                                                    actattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc                >  2:1953294/6‑150 (MQ=255)
                                                                                                                                    actattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc                >  1:1613680/6‑150 (MQ=255)
                                                                                                                                    aaTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc                >  2:625700/1‑150 (MQ=255)
                                                                                                                                    aaTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc                <  2:1789750/150‑1 (MQ=255)
                                                                                                                                     aTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggca               <  1:1483690/150‑1 (MQ=255)
                                                                                                                                     aTCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggca               >  2:688091/1‑150 (MQ=255)
                                                                                                                                       cGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCAcc             <  1:1866859/150‑1 (MQ=255)
                                                                                                                                       cGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCAcc             >  2:1730563/1‑150 (MQ=255)
                                                                                                                                          gCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                    >  1:1319115/1‑124 (MQ=255)
                                                                                                                                          gCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATg                                    <  2:1319115/124‑1 (MQ=255)
                                                                                                                                           cctaTAACCGGCAATAGTTCGACACGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCg         >  2:614191/4‑150 (MQ=255)
                                                                                                                                             gATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCggg       <  1:1760294/150‑1 (MQ=255)
                                                                                                                                               tAAGCGTCAATAGTTCGACAGGATGATGTATCTTCGGGAGGCGAATTCCGCCAGTCAGCACAATAAATATCGCCATTACCCTTAAGCTGCGCTTGCGTAATTTAATCTTTGTAAGAATGGCATTAAGGATGGCACTGGCACCAGCGGGAt     <  2:1651598/150‑1 (MQ=255)
                                                                                                                                                  ccGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCGCAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAgg  >  1:1306238/1‑150 (MQ=255)
                                                                                                                                                  ccGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAgg  <  1:627431/150‑1 (MQ=255)
                                                                                                                                                  ccGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAgg  >  1:321666/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGG  >  NC_000913/3241301‑3241596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑