Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,323,490 A→C 5.5% L194R (CTG→CGG)  glmM ← phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,323,4900AC 5.5% 279.6 / 5.1 87L194R (CTG→CGG) glmMphosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base A (41/41);  new base C (2/3);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGT  >  NC_000913/3323342‑3323639
                                                                                                                                                    |                                                                                                                                                     
atcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCgcag                                                                                                                                                      >  1:305750/1‑150 (MQ=255)
atcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCgcag                                                                                                                                                      <  1:1479985/150‑1 (MQ=255)
    ccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACg                                                                                                                                                  >  1:1415912/1‑150 (MQ=255)
       tCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTc                                                                                                                                               <  1:447879/150‑1 (MQ=255)
         gAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCgg                                                                                                                                             >  2:1451467/1‑150 (MQ=255)
           aGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCTCAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGgcg                                                                                                                                           >  1:1690750/1‑150 (MQ=255)
            ggCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGgcgc                                                                                                                                          >  1:596966/1‑150 (MQ=255)
             gCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGcgcg                                                                                                                                         >  2:1465893/1‑150 (MQ=255)
                aTACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtg                                                                                                                                      >  1:1571132/1‑150 (MQ=255)
                aTACCGAGATCCGCTCTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaac                                                                                                                                      <  1:1047097/150‑3 (MQ=255)
                 tACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaact                                                                                                                                     <  1:888293/150‑4 (MQ=255)
                 tACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtgt                                                                                                                                     <  2:1092277/150‑1 (MQ=255)
                   ccGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGa                                                                                                                                   >  1:1973320/1‑150 (MQ=255)
                   ccGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGa                                                                                                                                   <  1:184106/150‑1 (MQ=255)
                    cGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaactggg                                                                                                                                  <  1:250184/150‑7 (MQ=255)
                     gagaTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATACCGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATa                                                                                                                                 <  1:1931914/150‑1 (MQ=255)
                     gagaTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATa                                                                                                                                 <  2:1862604/150‑1 (MQ=255)
                         tCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTc                                                                                                                             >  2:144309/1‑150 (MQ=255)
                           cGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCTCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGc                                                                                                                           >  1:444917/1‑150 (MQ=255)
                           cGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGc                                                                                                                           <  2:123695/150‑1 (MQ=255)
                           cGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGc                                                                                                                           >  1:1986949/1‑150 (MQ=255)
                             cTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCAc                                                                                                                         <  1:191027/150‑1 (MQ=255)
                             cTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCAc                                                                                                                         <  1:752476/150‑1 (MQ=255)
                                    agccagcACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGc                                                                                                                  <  1:428060/150‑1 (MQ=255)
                                      ccagcACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGcac                                                                                                                <  1:1303748/150‑1 (MQ=255)
                                         gcACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAAt                                                                                                             >  1:1872442/1‑150 (MQ=255)
                                              cGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATccacc                                                                                                        <  2:1175933/150‑1 (MQ=255)
                                                aGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCcaccac                                                                                                      <  1:1465893/150‑1 (MQ=255)
                                                 gCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACcaca                                                                                                     >  2:1831046/1‑150 (MQ=255)
                                                    tGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCCATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTCATAAGTCGACCCGTTTCCACAATCAACAACAAta                                                                                                  >  2:629548/1‑149 (MQ=255)
                                                       aGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTc                                                                                               <  2:1415912/150‑1 (MQ=255)
                                                       aGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTc                                                                                               <  1:1568974/150‑1 (MQ=255)
                                                         cgcgcgAACGTCTGTAGCCGCGACTTCGGCATTGTTGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGAACAATCCACCACAATCTTCAg                                                                                             <  1:1933815/150‑1 (MQ=255)
                                                          gcgcgAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGt                                                                                            >  1:1748959/1‑150 (MQ=255)
                                                               aaCGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACt                                                                                       <  1:686114/150‑1 (MQ=255)
                                                                aCGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTg                                                                                      <  1:144309/150‑1 (MQ=255)
                                                                  gTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACAGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAg                                                                                    >  1:1319741/1‑150 (MQ=255)
                                                                        aGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAg                                                                              <  1:699639/150‑1 (MQ=255)
                                                                        aGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAg                                                                              >  1:1318556/1‑150 (MQ=255)
                                                                         gCCCCCACTTCGTCATTGATGTTTACACCGTTTGTCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTTATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCATTTCACTGAGGCTAAgt                                                                             <  2:936725/150‑1 (MQ=255)
                                                                         gCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgt                                                                             >  1:1591299/1‑150 (MQ=255)
                                                                          cccccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTATgtt                                                                            <  1:1491396/150‑1 (MQ=255)
                                                                            cccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcg                                                                          >  2:811428/1‑150 (MQ=255)
                                                                            cccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcg                                                                          >  1:1262131/1‑150 (MQ=255)
                                                                            cccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcg                                                                          >  1:1814030/1‑150 (MQ=255)
                                                                              cACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcgtt                                                                        <  2:351317/150‑1 (MQ=255)
                                                                                 ttCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCgg                                                                     >  1:1176200/1‑150 (MQ=255)
                                                                                  tCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCggg                                                                    <  2:444917/150‑1 (MQ=255)
                                                                                  tCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCggg                                                                    <  2:1986949/150‑1 (MQ=255)
                                                                                     gCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAAc                                                                 >  1:1427519/1‑150 (MQ=255)
                                                                                         tGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTgg                                                             <  2:596966/150‑1 (MQ=255)
                                                                                          gATGTTTACACCGTTTGGCTCACCACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGc                                                            >  2:1238440/1‑150 (MQ=255)
                                                                                          gATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGc                                                            <  2:6329/150‑1 (MQ=255)
                                                                                              tttACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTg                                                        >  1:1116065/1‑150 (MQ=255)
                                                                                               ttACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGc                                                       >  2:237841/1‑150 (MQ=255)
                                                                                                    ccGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAAc                                                  <  2:305750/150‑1 (MQ=255)
                                                                                                    ccGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAAc                                                  <  1:629548/150‑1 (MQ=255)
                                                                                                      gTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGc                                                       <  1:567746/143‑1 (MQ=255)
                                                                                                      gTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGc                                                       >  2:567746/1‑143 (MQ=255)
                                                                                                      gTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTc                                                <  1:1972549/150‑1 (MQ=255)
                                                                                                       tttGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAATCTCg                                               <  2:878332/150‑1 (MQ=255)
                                                                                                        ttGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGa                                              <  1:1451467/150‑1 (MQ=255)
                                                                                                        ttGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGa                                              >  2:927039/1‑150 (MQ=255)
                                                                                                        ttGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGa                                              <  2:1814030/150‑1 (MQ=255)
                                                                                                           gCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGatat                                           >  1:454119/1‑150 (MQ=255)
                                                                                                            cTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGAtata                                          >  2:638067/1‑150 (MQ=255)
                                                                                                             tCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAg                                         <  2:1973320/150‑1 (MQ=255)
                                                                                                              cacaACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGc                                        >  1:302686/1‑150 (MQ=255)
                                                                                                                    cGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCcg                                  <  2:1571132/150‑1 (MQ=255)
                                                                                                                         gCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCa                             >  1:1213860/1‑150 (MQ=255)
                                                                                                                         gCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCa                             >  1:808605/1‑150 (MQ=255)
                                                                                                                           gATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATc                           >  2:1775216/1‑150 (MQ=255)
                                                                                                                           gATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATc                           <  2:1591299/150‑1 (MQ=255)
                                                                                                                           gATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATc                           >  1:760846/1‑150 (MQ=255)
                                                                                                                            aTAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCa                          <  2:1213860/150‑1 (MQ=255)
                                                                                                                              aaCGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAAc                        <  2:1262131/150‑1 (MQ=255)
                                                                                                                                cGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGa                      <  1:237841/150‑1 (MQ=255)
                                                                                                                                     gCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACgg                 >  2:1998269/1‑150 (MQ=255)
                                                                                                                                      cccccAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCCCCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggc                >  1:696749/1‑150 (MQ=255)
                                                                                                                                        cccAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctg              >  2:157350/1‑150 (MQ=255)
                                                                                                                                          cAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctggc            >  1:996330/1‑150 (MQ=255)
                                                                                                                                           acatCGCGCCGAACGTGCTGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctggct           >  1:1663894/4‑150 (MQ=255)
                                                                                                                                           aGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctggct           <  2:1847834/150‑1 (MQ=255)
                                                                                                                                            gTTCGCGCCGCACGTTCGGCGCGATGTGATAAGGCGCACCGTTTGACCAATCCCCCACAAACTTCAGTTCACAGAGCCTAAGTTCGTTCGGGAACGTGGCTTGGCAAAACTCGATATAGCGACCCGCGCCAACAACGATACGGCTGGCtt          >  2:610108/1‑150 (MQ=255)
                                                                                                                                              tCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTa        <  2:212684/150‑1 (MQ=255)
                                                                                                                                              tCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTa        >  2:1588895/1‑150 (MQ=255)
                                                                                                                                                 cgcAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCCCCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTAccc     >  1:1848490/1‑150 (MQ=255)
                                                                                                                                                 cgcAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTAccc     <  2:1748959/150‑1 (MQ=255)
                                                                                                                                                 cgcAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTAccc     >  2:1648085/1‑150 (MQ=255)
                                                                                                                                                  gcagcaCGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCa    >  2:257195/1‑150 (MQ=255)
                                                                                                                                                    agcaCGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGt  >  2:1169043/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGT  >  NC_000913/3323342‑3323639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑