Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,345 A→T 6.6% A25A (GCT→GCA)  fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,3450AT 6.6% 240.2 / 4.9 75A25A (GCT→GCA) fkpApeptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base A (38/32);  new base T (2/3);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAC  >  NC_000913/3477196‑3477494
                                                                                                                                                     |                                                                                                                                                     
tCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTACATGTAACGACCCAGCGAGGCACCAAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTcagca                                                                                                                                                       >  2:1156686/1‑150 (MQ=255)
  cAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCagcagc                                                                                                                                                     >  2:1481187/1‑150 (MQ=255)
      ttGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCTGCTGAAGCTGCaaaa                                                                                                                                                 <  2:1333518/150‑1 (MQ=255)
       tGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCTGCTGAAGCTGCaaaac                                                                                                                                                <  2:1164178/150‑2 (MQ=255)
       tGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAg                                                                                                                                                <  2:149629/150‑1 (MQ=255)
          tGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGa                                                                                                                                             >  2:1557772/1‑150 (MQ=255)
           gCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTACCGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCTGCTGAAGCTGCaaaacctgc                                                                                                                                            <  1:663388/150‑6 (MQ=255)
           gCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAATGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAgcagca                                                                                                                                                    >  1:1973962/1‑142 (MQ=255)
           gCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAATGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAgcagca                                                                                                                                                    <  2:1973962/142‑1 (MQ=255)
           gCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGAt                                                                                                                                            <  1:571294/150‑1 (MQ=255)
              cAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTgg                                                                                                                                         <  2:1700587/150‑1 (MQ=255)
                gTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtg                                                                                                                                       <  2:834218/150‑1 (MQ=255)
                gTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtg                                                                                                                                       <  1:1415679/150‑1 (MQ=255)
                  ttttCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtgca                                                                                                                                     >  2:516481/1‑150 (MQ=255)
                   tttCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtgcat                                                                                                                                    <  1:467586/150‑1 (MQ=255)
                      cTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtgcatgca                                                                                                                                 <  2:1520921/150‑1 (MQ=255)
                      cTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGATGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtgcatgca                                                                                                                                 >  2:464529/1‑150 (MQ=255)
                            cTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCaa                                                                                                                           >  2:898779/1‑150 (MQ=255)
                                tAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGc                                                                                                                       >  1:304323/1‑150 (MQ=255)
                                tAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGc                                                                                                                       >  1:460368/1‑150 (MQ=255)
                                 agagagTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCGGCAGCAAAAGTGATTGGTGCATGCAGGGCAATGGcc                                                                                                                      >  2:43429/1‑150 (MQ=255)
                                     agTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATtg                                                                                                                  <  1:98718/150‑1 (MQ=255)
                                     agTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATtg                                                                                                                  >  1:1979891/1‑150 (MQ=255)
                                     agTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATtg                                                                                                                  <  1:998815/150‑1 (MQ=255)
                                          tCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTc                                                                                                             >  2:495214/1‑150 (MQ=255)
                                           ccATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCg                                                                                                            >  1:1910900/1‑150 (MQ=255)
                                             aTGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGcc                                                                                                          <  1:1419977/150‑1 (MQ=255)
                                             aTGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGcc                                                                                                          <  1:495214/150‑1 (MQ=255)
                                                tAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCcagc                                                                                                       >  2:1499320/1‑150 (MQ=255)
                                                      cccAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCggt                                                                                                 <  1:898779/150‑3 (MQ=255)
                                                      cccAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGtt                                                                                                 >  2:1711787/1‑150 (MQ=255)
                                                        cAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTAc                                                                                               >  2:1387087/1‑150 (MQ=255)
                                                        cAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTAc                                                                                               <  1:1156686/150‑1 (MQ=255)
                                                        cAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGCGCAACGGCCATTGTGGTCGCCAGCAGCGTTAc                                                                                               >  2:1144301/1‑150 (MQ=255)
                                                         aGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACt                                                                                              >  2:1768718/1‑150 (MQ=255)
                                                         aGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACt                                                                                              >  1:333314/1‑150 (MQ=255)
                                                             aGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGATGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTaa                                                                                          <  1:464529/150‑1 (MQ=255)
                                                              ggCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTaaa                                                                                         >  1:714541/1‑150 (MQ=255)
                                                               gCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAAc                                                                                        >  1:1795565/1‑150 (MQ=255)
                                                                   ccAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTg                                                                                    <  2:1099105/150‑1 (MQ=255)
                                                                        gCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTc                                                                               <  1:1387087/150‑1 (MQ=255)
                                                                           tAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATc                                                                            <  1:516481/150‑1 (MQ=255)
                                                                             aGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCa                                                                          <  1:1768718/150‑1 (MQ=255)
                                                                              gCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGTGTTACTTTAAACAGTGATTTCATCCat                                                                         >  1:1413174/1‑150 (MQ=255)
                                                                               cTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCata                                                                        <  2:1432358/150‑1 (MQ=255)
                                                                               cTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCata                                                                        <  1:179638/150‑1 (MQ=255)
                                                                                 gATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATAtc                                                                      >  1:99970/1‑150 (MQ=255)
                                                                                     tCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCa                                                                  >  1:1340678/1‑150 (MQ=255)
                                                                                      cTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAg                                                                 <  2:108269/150‑1 (MQ=255)
                                                                                      cTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAg                                                                 >  2:1603845/1‑150 (MQ=255)
                                                                                         aTCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGAt                                                              >  1:209107/1‑150 (MQ=255)
                                                                                          tCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATc                                                             <  2:99970/150‑1 (MQ=255)
                                                                                            gTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCgg                                                           <  2:304323/150‑1 (MQ=255)
                                                                                             tCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCggg                                                          >  2:1250035/1‑150 (MQ=255)
                                                                                              cATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCgggg                                                         <  1:1481187/150‑1 (MQ=255)
                                                                                                      aaCGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAc                                                 <  2:1795565/150‑1 (MQ=255)
                                                                                                            gctTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGTGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAgg                                           <  2:1413174/150‑1 (MQ=255)
                                                                                                                  cTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAAc                                     >  2:1672830/1‑150 (MQ=255)
                                                                                                                       agcagcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtatta                                >  1:1244988/1‑150 (MQ=255)
                                                                                                                       agcagcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtatta                                >  2:1593285/1‑150 (MQ=255)
                                                                                                                       agcagcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtatta                                <  1:631966/150‑1 (MQ=255)
                                                                                                                         cagcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATc                              >  1:449659/1‑150 (MQ=255)
                                                                                                                           gcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAg                            >  1:1929014/1‑150 (MQ=255)
                                                                                                                            cTGTAGCAGGTTTTGCAGCTTCGGCAGCAAAAGTGATTGGTGCATGCAGGGCAATGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGa                           <  1:43429/150‑1 (MQ=255)
                                                                                                                              gTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAg                         >  1:373289/1‑150 (MQ=255)
                                                                                                                              gTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAg                         >  1:879647/1‑150 (MQ=255)
                                                                                                                              gTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAg                         <  2:1910900/150‑1 (MQ=255)
                                                                                                                                aGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAggg                       >  2:708093/1‑150 (MQ=255)
                                                                                                                                 gCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGt                      >  1:1839795/1‑150 (MQ=255)
                                                                                                                                  cAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTa                     >  2:1471937/1‑150 (MQ=255)
                                                                                                                                   aGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTAc                    <  1:1557772/150‑1 (MQ=255)
                                                                                                                                    ggTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACt                   <  2:209107/150‑1 (MQ=255)
                                                                                                                                    ggTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACt                   >  1:16591/1‑150 (MQ=255)
                                                                                                                                    ggTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACt                   >  2:1831472/1‑150 (MQ=255)
                                                                                                                                     cttttGCTGCTGAAGCTGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACta                  >  2:474536/2‑150 (MQ=255)
                                                                                                                                     cttttGCTGCTGAAGCTGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACta                  >  2:481645/2‑150 (MQ=255)
                                                                                                                                            aGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCg           >  2:987607/1‑150 (MQ=255)
                                                                                                                                               ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg        <  2:1979891/150‑1 (MQ=255)
                                                                                                                                               ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg        >  2:908754/1‑150 (MQ=255)
                                                                                                                                                 cagcagCAAAAGTGATTGTTTCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc      <  1:852253/150‑1 (MQ=255)
                                                                                                                                                 cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc      >  2:1964851/1‑150 (MQ=255)
                                                                                                                                                   gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa    <  1:1603845/150‑1 (MQ=255)
                                                                                                                                                    cagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGaa   >  1:167774/1‑150 (MQ=255)
                                                                                                                                                     agcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc  <  1:463228/150‑1 (MQ=255)
                                                                                                                                                     agcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc  <  2:460368/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAC  >  NC_000913/3477196‑3477494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑