Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,560,903 A→T 5.4% L184I (TTA→ATA)  glpG ← rhomboid protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,9030AT 5.4% 236.0 / 3.0 74L184I (TTA→ATA) glpGrhomboid protease
Reads supporting (aligned to +/- strand):  ref base A (37/33);  new base T (2/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACT  >  NC_000913/3560764‑3561052
                                                                                                                                           |                                                                                                                                                     
aTTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTg                                                                                                                                             >  1:31635/1‑150 (MQ=255)
aTTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTg                                                                                                                                             >  1:24308/1‑150 (MQ=255)
  tAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGAt                                                                                                                                           <  1:1971892/150‑1 (MQ=255)
      ccACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGc                                                                                                                                       >  2:1992057/1‑150 (MQ=255)
       cACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTATCAGCGCCCTGTTAAGCg                                                                                                                                      <  1:458672/150‑1 (MQ=255)
       cACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCg                                                                                                                                      <  2:124716/150‑1 (MQ=255)
         cGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTATCAGCGCCCTGTTAAGCggc                                                                                                                                    <  2:1159163/150‑3 (MQ=255)
            tGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATg                                                                                                                                 >  1:1337126/1‑150 (MQ=255)
            tGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATg                                                                                                                                 >  1:1598980/1‑150 (MQ=255)
                                 ggATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCg                                                                                                            >  2:865415/1‑150 (MQ=255)
                                           cGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGttttt                                                                                                  >  2:1083670/1‑150 (MQ=255)
                                             ccACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGCATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTcc                                                                                                >  2:719878/1‑150 (MQ=255)
                                                cGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCcacc                                                                                             <  2:1504311/150‑1 (MQ=255)
                                                  tAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGtttt                                                                                                   <  1:925043/142‑1 (MQ=255)
                                                  tAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGtttt                                                                                                   >  2:925043/1‑142 (MQ=255)
                                                    gCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCcaccgcac                                                                                         >  2:736844/1‑150 (MQ=255)
                                                    gCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCcaccgcac                                                                                         >  2:743700/1‑150 (MQ=255)
                                                       aGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCaccgcaccgc                                                                                      <  2:1537931/150‑1 (MQ=255)
                                                         aCGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCAccgccg                                                                                    >  2:1111529/1‑150 (MQ=255)
                                                           gTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCgag                                                                                  <  1:152432/150‑1 (MQ=255)
                                                            tAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCgaga                                                                                 >  1:1845845/1‑150 (MQ=255)
                                                                ccATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATacc                                                                             <  2:1845845/150‑1 (MQ=255)
                                                                ccATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATacc                                                                             <  1:836620/150‑1 (MQ=255)
                                                                ccATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGAACTCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATacc                                                                             >  2:981148/1‑150 (MQ=255)
                                                                 cATCAGCGCATACACCACGCTAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATacca                                                                            >  1:253129/1‑150 (MQ=255)
                                                                 cATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCcac                                                                                              <  2:1677817/132‑1 (MQ=255)
                                                                 cATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCcac                                                                                              >  1:1677817/1‑132 (MQ=255)
                                                                 cATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATaccc                                                                            >  2:842974/1‑149 (MQ=255)
                                                                 cATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATacca                                                                            >  2:486572/1‑150 (MQ=255)
                                                                     aGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATaccaccac                                                                        >  1:1016373/1‑150 (MQ=255)
                                                                      gcgcATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATaccaccacc                                                                       >  1:1931994/1‑150 (MQ=255)
                                                                       cgcATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATAccaccacca                                                                      <  2:1016373/150‑1 (MQ=255)
                                                                        gcATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCag                                                                     <  1:776360/150‑1 (MQ=255)
                                                                        gcATACACCACGCCAGAAAGCCCGCCAAACAACGGCCAGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCag                                                                     >  2:1615244/1‑150 (MQ=255)
                                                                        gcATACACAACGCAAGAAAGCCCGCCAAACCACGGCCAGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCag                                                                     <  2:531125/150‑1 (MQ=255)
                                                                          aTACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTAAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCagag                                                                   >  2:1885028/1‑150 (MQ=255)
                                                                               ccacGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGt                                                              >  1:879181/1‑150 (MQ=255)
                                                                               ccacGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGt                                                              >  2:119504/1‑150 (MQ=255)
                                                                               ccacGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGt                                                              <  1:1199975/150‑1 (MQ=255)
                                                                                  cGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTaa                                                           <  1:1992057/150‑1 (MQ=255)
                                                                                   gCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTaaa                                                          >  1:1604268/1‑150 (MQ=255)
                                                                                     cAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAaga                                                        >  2:1334944/1‑150 (MQ=255)
                                                                                      aGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAagag                                                       <  1:1111529/150‑1 (MQ=255)
                                                                                       gAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAgg                                                      <  1:486572/150‑1 (MQ=255)
                                                                                          aGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGata                                                   <  2:1428957/150‑1 (MQ=255)
                                                                                          aGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGata                                                   <  1:635303/150‑1 (MQ=255)
                                                                                              cGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCa                                               <  1:345244/150‑1 (MQ=255)
                                                                                               gCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCAt                                              >  1:74751/1‑150 (MQ=255)
                                                                                                 cAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCa                                            >  1:1172270/1‑150 (MQ=255)
                                                                                                     ccACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGCTACCACCACCAGAGCAGGTTAAAGAGGATATGCCTCAGCga                                        >  2:441302/1‑150 (MQ=255)
                                                                                                       aCGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCgaga                                      <  1:1908095/150‑1 (MQ=255)
                                                                                                          gCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGt                                   <  2:382753/150‑1 (MQ=255)
                                                                                                               cTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCAtt                              <  1:743700/150‑1 (MQ=255)
                                                                                                               cTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCAtt                              >  2:1803348/1‑150 (MQ=255)
                                                                                                               cTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCAtt                              <  1:736844/150‑1 (MQ=255)
                                                                                                                 gAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTaa                            >  2:1707778/1‑150 (MQ=255)
                                                                                                                   aTTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTg                                  <  2:1923265/142‑1 (MQ=255)
                                                                                                                   aTTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTg                                  >  1:1923265/1‑142 (MQ=255)
                                                                                                                   aTTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAAcg                          <  1:1620803/150‑1 (MQ=255)
                                                                                                                   aTTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAAcg                          <  1:222509/150‑1 (MQ=255)
                                                                                                                       ttGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTg                      <  2:24308/150‑1 (MQ=255)
                                                                                                                       ttGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTg                      <  2:31635/150‑1 (MQ=255)
                                                                                                                           tgcACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTg                  >  2:93553/1‑150 (MQ=255)
                                                                                                                             cactTAGCCGCTTAACAGGGCGGTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCAGCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGGGTGGGTGaa                <  2:1122367/150‑1 (MQ=255)
                                                                                                                             cacaTAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGaa                >  1:1441976/1‑150 (MQ=255)
                                                                                                                                cattacGCTTATCAGCGCCCTGTTAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTa             >  1:1958694/6‑150 (MQ=255)
                                                                                                                                aTAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTa             >  1:279908/1‑150 (MQ=255)
                                                                                                                                   gCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACg          <  1:719878/150‑1 (MQ=255)
                                                                                                                                    acGCTTATCAGCGCCCTGTTAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGc         >  1:1619435/2‑150 (MQ=255)
                                                                                                                                      gCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGaa                                     >  2:272488/1‑120 (MQ=255)
                                                                                                                                      gCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGaa                                     <  1:272488/120‑1 (MQ=255)
                                                                                                                                      gCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTggg                    <  2:344605/137‑1 (MQ=255)
                                                                                                                                      gCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTggg                    >  1:344605/1‑137 (MQ=255)
                                                                                                                                      gCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCa       <  1:1334944/150‑1 (MQ=255)
                                                                                                                                          aaCAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAAc   >  2:1892252/1‑150 (MQ=255)
                                                                                                                                           aCAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACt  >  2:1844643/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                     
ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACT  >  NC_000913/3560764‑3561052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑